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A method for trapping live tardigrade mosquitoes and raising them in large quantities indoors

A technology of tardy-eyed fungus and live mosquitoes, which is applied in the trapping of live tardy-eyed fungus and large-scale indoor breeding, which can solve the problems of cumbersome operation, damage to adults, damage to seedlings, etc., and achieve high survival rate and simple operation , low cost effect

Active Publication Date: 2020-05-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The larva collection is generally collected directly from the larvae that are inflicting damage on the damaged plant or the larvae in the soil near the damaged plant. Since the larvae of T. tardiformis have the habit of moving quickly and concealing when they are disturbed, and the larvae are small, if it occurs Not very serious plots, it is extremely time-consuming and laborious to find more initial larvae
Adults are usually collected with a sucker, which will cause damage to the adults and prevent them from reproducing normally. Because the female adults have weak flying ability, they are easier to catch, while the male adults are more capable of flying, so it is difficult to catch them with the sucker. , and it is not easy to buy an insect sucker suitable for such small insects. There is even a lack of materials and production technology for self-made insect suckers. How to place the adult insects sucked by the insect sucker in an environment suitable for their survival and laying eggs without escaping is also facing the problem
The tardy-eyed fungus occurs in greenhouse trees and flowers. One to a few relatively small larvae on one plant eat the underground stem, which can make the seedling unable to take root and seriously affect the emergence rate. After eating one plant, the larvae move to cause damage or Scatter into the soil to feed on humus, so it is more difficult to find many initial larvae in the greenhouse. When looking for the source of insects, it is necessary to pull out the seedlings. Reduced seedling survival rate
[0005] Some of the current breeding and breeding technologies for tardigrade gnats are feeding with fresh host feed, which needs to be replaced every day or every other day, or needs to be fed with edible fungus mycelium culture medium, which is technically difficult, or artificially synthesized feed, which needs to remove food Residue and excrement, there are problems such as cumbersome operation, time-consuming and labor-intensive

Method used

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  • A method for trapping live tardigrade mosquitoes and raising them in large quantities indoors
  • A method for trapping live tardigrade mosquitoes and raising them in large quantities indoors
  • A method for trapping live tardigrade mosquitoes and raising them in large quantities indoors

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Experimental program
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Effect test

Embodiment 1

[0065] (1) Preparation of petri dish for raising: spread qualitative filter paper with a diameter of 9 cm on the bottom layer of a disposable plastic petri dish with a lid diameter of 9 cm, add tap water to the filter paper with a 10-15 cm long plastic dropper, and use another dry Blot the open water on the filter paper in the petri dish with filter paper, set aside (such as Figure 5 shown).

[0066] (2) Preparation of peat soil for raising: take the imported peat soil purchased on the flower market and take the amount required for the test and mash it, add tap water to prepare peat soil with a relative humidity of 60-65%, and put it into a tissue culture tissue with a diameter of 7 cm and a height of 10 cm. In the bottle, the filling volume is 3 / 4 of the volume of the tissue culture bottle, put the lid on, and put it in an autoclave, at 121°C, 1.1kg / cm 2 Sterilize under pressure for 40 minutes, take it out and place it at room temperature for later use (such as Figure 4 s...

Embodiment 2

[0082] Preparation of unmated male and female adults: The adults of Bradyphagia tardiformis are small, unlike large insects. Female adults and male adults can be simply placed together for experimental observation such as mating. The artificial rearing of larvae is generally carried out in a petri dish with a diameter of 9 cm, and the offspring male and female adults from one female adult occur in the same petri dish, which is very easy to cause inbreeding. Single pupae were reared in separate petri dishes when the pupae stage occurred (e.g. Figure 10 shown), pupal eclosion can obtain unmated female or male adults.

[0083] How to use the adult test device: Put a pair of petri dishes with female and male adults into the wide-mouth container 1 from the opening end of the white cotton cloth 2 of the adult test device, open the cover of the petri dish, let the adults fly into the adult test device, and take out the petri dish , and then tighten the calico cloth 2 open ends, you...

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Abstract

The invention discloses a method for trapping bradysia sp. living insects and carrying out indoor large-scale feeding, and belongs to the technical fields of agricultural scientific research and insect breeding. According to the invention, the method for trapping bradysia sp. living insects is characterized in that a fat pork and pleurotus geesteranus are buried in a small flowerpot for inducing bradysia sp. female adults to lay eggs, and the eggs are incubated for obtaining a large amount of larvae as initial test insects. The method is simple and feasible, and a large amount of the initial test insects can be easily obtained. According to the breeding method provided by the invention, peat soil is used as a feeding environment, pleurotus geesteranus serves as a feed, and the feed does not need to be replaced and excrement does not need to be cleaned in a development period, so that time and labor are saved, test materials are easy to obtain, cost is low, the operation is simple, various test observations can be conveniently carried out, inbreeding of offspring can be avoided, and the population is prevented from degradation caused by the inbreeding during the breeding throughoutthe year. The survival rates of all insect states are high, and 80% of eggs or more can be developed into adults. Therefore, a large amount of larvae can be trapped in a short time to serve as initialinsect sources, and annual large-scale breeding of the bradysia sp. is realized.

Description

technical field [0001] The invention belongs to the field of agricultural scientific research technology and insect breeding technology, and in particular relates to a method for trapping and indoor mass breeding of live fungus tardigrades. Background technique [0002] Bradysia sp. belongs to Diptera, Longocera, Mycoidea, the largest genus Bradysia Winnertz pest in the family Oculidae, 455 known species in the world , my country has recorded 131 species. Tardyphagia mainly uses larvae to damage the young underground tissues of Liliaceae plants represented by leeks, the mycelium and fruiting body primordium of wild and cultivated edible fungi, and the young underground stems and leaves of greenhouse garden flower plants. The tardigrade larvae that harm leeks mainly feed on the pseudostems, bulbs, leaf sheaths, young stems and shoots of leeks, causing the underground parts of leeks to rot, the leaves above the ground are thin and yellow, and even the whole plant dies, serious...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30
CPCA01K67/033A23K10/30A23K50/90
Inventor 孙朝辉马涛梁永婵朱映路艺史先慧邓小梅王偲温秀军
Owner SOUTH CHINA AGRI UNIV