A method for trapping live tardigrade mosquitoes and raising them in large quantities indoors
A technology of tardy-eyed fungus and live mosquitoes, which is applied in the trapping of live tardy-eyed fungus and large-scale indoor breeding, which can solve the problems of cumbersome operation, damage to adults, damage to seedlings, etc., and achieve high survival rate and simple operation , low cost effect
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Embodiment 1
[0065] (1) Preparation of petri dish for raising: spread qualitative filter paper with a diameter of 9 cm on the bottom layer of a disposable plastic petri dish with a lid diameter of 9 cm, add tap water to the filter paper with a 10-15 cm long plastic dropper, and use another dry Blot the open water on the filter paper in the petri dish with filter paper, set aside (such as Figure 5 shown).
[0066] (2) Preparation of peat soil for raising: take the imported peat soil purchased on the flower market and take the amount required for the test and mash it, add tap water to prepare peat soil with a relative humidity of 60-65%, and put it into a tissue culture tissue with a diameter of 7 cm and a height of 10 cm. In the bottle, the filling volume is 3 / 4 of the volume of the tissue culture bottle, put the lid on, and put it in an autoclave, at 121°C, 1.1kg / cm 2 Sterilize under pressure for 40 minutes, take it out and place it at room temperature for later use (such as Figure 4 s...
Embodiment 2
[0082] Preparation of unmated male and female adults: The adults of Bradyphagia tardiformis are small, unlike large insects. Female adults and male adults can be simply placed together for experimental observation such as mating. The artificial rearing of larvae is generally carried out in a petri dish with a diameter of 9 cm, and the offspring male and female adults from one female adult occur in the same petri dish, which is very easy to cause inbreeding. Single pupae were reared in separate petri dishes when the pupae stage occurred (e.g. Figure 10 shown), pupal eclosion can obtain unmated female or male adults.
[0083] How to use the adult test device: Put a pair of petri dishes with female and male adults into the wide-mouth container 1 from the opening end of the white cotton cloth 2 of the adult test device, open the cover of the petri dish, let the adults fly into the adult test device, and take out the petri dish , and then tighten the calico cloth 2 open ends, you...
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