Fluorescence carbon spot as well as preparation method and application thereof
A technology of fluorescent carbon dots and leaves, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problems of complex preparation methods of carbon dots, difficulty in long-term use, and danger, and achieve good optical detection ability, Uniform dispersion and resource saving effect
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Embodiment 1
[0024] (1) Pretreatment of sweet-scented osmanthus leaves:
[0025] Pick some fresh sweet-scented osmanthus leaves, wash them several times with tap water, and then wash them with deionized water; put the washed sweet-scented osmanthus leaves in an oven at 40°C and dry them for one week; crush them with a pulverizer, and sieve them through a 150-mesh sieve , to obtain the osmanthus leaf powder, set aside.
[0026] (2) Preparation of fluorescent carbon dot dispersion:
[0027] Weigh 1 g of osmanthus leaf powder in a round bottom flask, add 40 mL of deionized water, add 0.5 mL of polyethyleneimine solution with magnetic stirring, and magnetically stir for 24 h; centrifuge to collect the supernatant; in order to remove insoluble impurities and unreacted The supernatant collected by centrifugation was filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 to obtain a fluorescent carbon dot dispers...
Embodiment 2
[0029] (1) Pretreatment of sweet-scented osmanthus leaves:
[0030] Pick some fresh sweet-scented osmanthus leaves, wash them several times with tap water, and then wash them with deionized water; place the washed sweet-scented osmanthus leaves in an oven at 50°C, and dry them for one week; crush them with a pulverizer, and sieve them through a 150-mesh sieve , to obtain the osmanthus leaf powder, set aside.
[0031] (2) Preparation of fluorescent carbon dot dispersion:
[0032] Weigh 1 g of sweet-scented osmanthus leaf powder in a round bottom flask, add 80 mL of deionized water, add 2 mL of polyethyleneimine solution with magnetic stirring, and magnetically stir for 36 h; centrifuge to collect the supernatant; in order to remove insoluble impurities and unreacted The supernatant collected by centrifugation was filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 to obtain a fluorescent car...
Embodiment 3
[0034] (1) Pretreatment of sweet-scented osmanthus leaves:
[0035] Pick some fresh sweet-scented osmanthus leaves, wash them several times with tap water, and then wash them with deionized water; place the washed sweet-scented osmanthus leaves in an oven at 60°C, and dry them for one week; crush them with a pulverizer, and sieve them through a 150-mesh sieve , to obtain the osmanthus leaf powder, set aside.
[0036] (2) Preparation of fluorescent carbon dot dispersion:
[0037] Weigh 1 g of osmanthus leaf powder in a round bottom flask, add 60 mL of deionized water, add 1 mL of polyethyleneimine solution with magnetic stirring, and magnetically stir for 48 h; centrifuge to collect the supernatant; in order to remove insoluble impurities and unreacted The supernatant collected by centrifugation was filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 to obtain a fluorescent carbon dot disper...
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