Fluorescence carbon spot as well as preparation method and application thereof

A technology of fluorescent carbon dots and leaves, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problems of complex preparation methods of carbon dots, difficulty in long-term use, and danger, and achieve good optical detection ability, Uniform dispersion and resource saving effect

Inactive Publication Date: 2018-12-21
JIANGSU UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the technical defects existing in the prior art, such as: in the existing method for synthesizing carbon dots, most of the carbon sources need to be purchased (such as: carbon nanotubes, graphite powder, glucose phenolic resin, etc.), while , the method for preparing carbon dots is relatively complicated, some even have dangers, and are not easy to use for a long time. The present invention provides a method for preparing fluorescent carbon dots by using environmentally friendly and widely sourced sweet-scented osmanthus leaves as a carbon source and using a simple solvent extraction method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence carbon spot as well as preparation method and application thereof
  • Fluorescence carbon spot as well as preparation method and application thereof
  • Fluorescence carbon spot as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Pretreatment of sweet-scented osmanthus leaves:

[0025] Pick some fresh sweet-scented osmanthus leaves, wash them several times with tap water, and then wash them with deionized water; put the washed sweet-scented osmanthus leaves in an oven at 40°C and dry them for one week; crush them with a pulverizer, and sieve them through a 150-mesh sieve , to obtain the osmanthus leaf powder, set aside.

[0026] (2) Preparation of fluorescent carbon dot dispersion:

[0027] Weigh 1 g of osmanthus leaf powder in a round bottom flask, add 40 mL of deionized water, add 0.5 mL of polyethyleneimine solution with magnetic stirring, and magnetically stir for 24 h; centrifuge to collect the supernatant; in order to remove insoluble impurities and unreacted The supernatant collected by centrifugation was filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 to obtain a fluorescent carbon dot dispers...

Embodiment 2

[0029] (1) Pretreatment of sweet-scented osmanthus leaves:

[0030] Pick some fresh sweet-scented osmanthus leaves, wash them several times with tap water, and then wash them with deionized water; place the washed sweet-scented osmanthus leaves in an oven at 50°C, and dry them for one week; crush them with a pulverizer, and sieve them through a 150-mesh sieve , to obtain the osmanthus leaf powder, set aside.

[0031] (2) Preparation of fluorescent carbon dot dispersion:

[0032] Weigh 1 g of sweet-scented osmanthus leaf powder in a round bottom flask, add 80 mL of deionized water, add 2 mL of polyethyleneimine solution with magnetic stirring, and magnetically stir for 36 h; centrifuge to collect the supernatant; in order to remove insoluble impurities and unreacted The supernatant collected by centrifugation was filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 to obtain a fluorescent car...

Embodiment 3

[0034] (1) Pretreatment of sweet-scented osmanthus leaves:

[0035] Pick some fresh sweet-scented osmanthus leaves, wash them several times with tap water, and then wash them with deionized water; place the washed sweet-scented osmanthus leaves in an oven at 60°C, and dry them for one week; crush them with a pulverizer, and sieve them through a 150-mesh sieve , to obtain the osmanthus leaf powder, set aside.

[0036] (2) Preparation of fluorescent carbon dot dispersion:

[0037] Weigh 1 g of osmanthus leaf powder in a round bottom flask, add 60 mL of deionized water, add 1 mL of polyethyleneimine solution with magnetic stirring, and magnetically stir for 48 h; centrifuge to collect the supernatant; in order to remove insoluble impurities and unreacted The supernatant collected by centrifugation was filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 to obtain a fluorescent carbon dot disper...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
sizeaaaaaaaaaa
Login to view more

Abstract

The invention specifically relates to a fluorescence carbon spot as well as a preparation method and application thereof and belongs to the field of novel nano material preparation. The fluorescence carbon spot preparation method disclosed by the invention comprises the specific steps: cleaning leaves, putting in an oven to be dried and smashing and screening to obtain leaf powder; adding deionized water into the leaf powder, then adding a polyethyleneimine solution into a magnetic stirring process and continuing magnetically stirring under the room temperature; centrifuging, collecting supernatant and filtering and dialyzing the supernatant to obtain fluorescence carbon spot dispersion liquid. The preparation method disclosed by the invention has simpleness, convenience and safety; meanwhile, use of expensive instruments is reduced; the raw materials are low in cost, environmentally friendly and renewable. The fluorescence carbon spot disclosed by the invention has the advantages of uniform particle size and evenness in dispersion; the fluorescence carbon spot has high detection flexibility to bovine hemoglobin, a linear range is 0 to 54 mu g / L, a complex modified process is avoided, a detection method is simple, and good optical detection capacity is achieved. The fluorescence carbon spot disclosed by the invention has a wide application prospect in detecting bovine hemoglobin.

Description

technical field [0001] The invention specifically relates to a fluorescent carbon dot and its preparation method and application, and belongs to the field of preparation of new nanometer materials. Background technique [0002] Protein is the main bearer of life activities and a biological macromolecule with a three-dimensional spatial structure. The normal expression of protein is closely related to the health of living organisms. Among many types of proteins, hemoglobin has an important physiological function as a carrier of oxygen transport in the body due to its unique oxygen-binding properties. The content of hemoglobin in the blood is closely related to many clinical diseases, such as anemia, leukemia and heart disease. Bovine hemoglobin is a respiratory protein in the red blood cells of vertebrates. It plays the role of transporting oxygen, decomposing hydrogen peroxide, and transferring electrons in the living body. It has similar functions to human hemoglobin. Afte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C09K11/65G01N21/64B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00C09K11/65C01B32/15G01N21/6428
Inventor 王文娟刘锡清闫永胜李春香
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap