Method for screening almond-flavored tea tree germplasm resources

A screening method and tea tree technology are applied in the field of screening of almond fragrant tea tree germplasm resources, which can solve the problems of labor-intensive and time-consuming, heavy workload, slow screening speed, etc. Effect
CN109060995AActive Publication Date: 2018-12-21TEA RES INST GUANGDONG ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TEA RES INST GUANGDONG ACAD OF AGRI SCI
Publication Date
2018-12-21

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Abstract

The invention discloses a method for screening almond-flavored tea tree germplasm resources. The method comprises the following steps of selecting three parts of tea raw materials, wherein the two parts have the almond-flavored tea tree germplasm resources, and the rest part does not have the almond-flavored tea tree germplasm resources; weighing 50-70 mg of a sample, putting the sample into a grinding machine for grinding, taking out the sample, and carrying out ultrasonic extraction for 20-40 minutes; adding 100-300 [mu]L of chloroform into the sample, performing uniform mixing, adding water, performing uniform mixing, and carrying out ultrasonic extraction for 10-30 minutes; adding a methoxyamine hydrochloride pyridine solution into a glass-derived small bottle, carrying out vortex oscillation for 2 minutes, carrying out oximation reaction in a concussion incubator, taking out the sample, and placing the sample at the room temperature for 30 minutes; and collecting sample data to perform GC-MS metabonomics analysis. Through the technical scheme, the content of mandelonitrile in tea leaf metabolites can be rapidly analyzed, so that the screening work of the almond-flavored tea tree germplasm resources can be rapidly realized, and the working time of original 4-year breeding is greatly shortened.
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Description

technical field

[0001] The invention relates to the technical field of tea planting, in particular to a method for screening germplasm resources of almond-flavored tea trees. Background technique

[0002] "Almond Fragrance" black tea is black tea made from a special tea tree raw material. The finished tea has an almond milk aroma. It is extremely difficult to breed varieties with high quality resources.

[0003] The current screening method is to collect fresh leaves from a single plant or to process and trial-produce black tea after cutting and breeding a single plant, but these two methods have a huge workload and the screening speed is relatively slow. Using the method of collecting fresh leaves from a single plant for processing and trial production, due to the small number of tea greens in a single tea tree, processing is difficult, and the original characteristics of the germplasm resources cannot be maintained, so tea tree germplasm resources with certain characteris...

Claims

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