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A heterotrophic nitrification-aerobic denitrification compound bacterial agent capable of removing high ammonia nitrogen at low temperature and its application

A compound bacterial agent and heterotrophic nitrification technology, applied in the field of microorganisms, can solve the problems of poor wastewater treatment quality, high treatment cost and low treatment efficiency, and achieve the effects of strong tolerance, high treatment efficiency and low treatment cost.

Active Publication Date: 2022-03-18
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large amount of free ammonia contained in high-concentration ammonia-nitrogen wastewater has a strong inhibitory effect on autotrophic nitrifying bacteria, the ammonia oxidation process of autotrophic nitrifying bacteria is inhibited, affecting the efficiency of ammonia nitrogen treatment, resulting in these biological methods in the treatment of high The treatment effect of ammonia nitrogen wastewater is not good; in addition, the optimum growth temperature range of autotrophic nitrifying bacteria is 25~35°C. When the temperature is lower than 10°C, the growth of autotrophic nitrifying bacteria stops, which seriously affects the nitrification reaction, resulting in poor wastewater treatment quality, Dealing with problems such as low efficiency and high processing costs

Method used

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  • A heterotrophic nitrification-aerobic denitrification compound bacterial agent capable of removing high ammonia nitrogen at low temperature and its application
  • A heterotrophic nitrification-aerobic denitrification compound bacterial agent capable of removing high ammonia nitrogen at low temperature and its application
  • A heterotrophic nitrification-aerobic denitrification compound bacterial agent capable of removing high ammonia nitrogen at low temperature and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Step 1) Acclimatization of strains

[0052] Copper greedy bacteria Cupriavidus Acclimatization of sp. SWA1 low temperature and high ammonia nitrogen tolerance: Inoculate 2 mL of bacterial liquid into a 250 mL Erlenmeyer flask filled with 100 mL of sterilized heterotrophic nitrification medium, shake well, 5°C, 150 r min -1 Cultured under the same conditions for 2 days. Continuously subcultured under the same conditions above, a total of 5 times, corresponding to ammonia nitrogen concentrations of 200 mg / L, 300 mg / L, 400 mg / L, 500 mg / L, and 600 mg / L.

[0053] Alcaligenes faecalis Alcaligenes faecalis (CICC 22642) Low temperature, high ammonia nitrogen tolerance acclimatization: Inoculate 2 mL of bacterial liquid into a 250 mL Erlenmeyer flask containing 100 mL of sterilized heterotrophic nitrification medium, shake well, 5°C, 150r min -1 Cultured under the same conditions for 2 days. Continuously subculture under the same conditions as above, and continuously incre...

Embodiment 2

[0068] Example 2: Experiment of removal of high ammonia nitrogen by compound bacterial agent at 5°C

[0069] Prepare a heterotrophic nitrification medium with an ammonia nitrogen concentration of 400 mg / L, take 100 mL of heterotrophic nitrification culture based on a 250 mL Erlenmeyer flask, and add 2 mL of compound bacterial agent bacterial solution (OD600 is 1 -2), sealed with parafilm, placed in a shaker set at 5°C, 150 r min -1 conditions, and then measure the OD600 value of the bacterial solution every 24 h to determine the growth of the bacteria, and measure the content of ammonia nitrogen and total nitrogen in the medium at the same time to determine the removal effect of ammonia nitrogen and total nitrogen. figure 1 It can be seen that the ammonia nitrogen removal rate of the compound bacterial agent can reach 100% and the total nitrogen removal rate can reach 79.05% at 5°C.

Embodiment 3

[0070] Example 3: The experiment of removing high ammonia nitrogen by compound bacterial agent at different temperatures

[0071] Prepare a heterotrophic nitrification medium with an ammonia nitrogen concentration of 400 mg / L, take 100 mL of heterotrophic nitrification culture based on a 250 mL Erlenmeyer flask, and add 2 mL of compound bacterial agent bacterial solution (OD600 is 1 -2), sealed with parafilm, put different Erlenmeyer flasks into different shakers (temperature settings were 5°C, 10°C, 15°C, 20°C, 25°C, 35°C, speed setting 150 r min -1 ), and then measure the OD600 value of the bacterial solution every 24 h to determine the growth of the bacteria, and at the same time measure the content of ammonia nitrogen in the medium to determine the removal effect of ammonia nitrogen. Depend on figure 2 It can be seen that the compound microbial agent can achieve efficient removal of ammonia nitrogen in the range of 5-35 °C.

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Abstract

The invention relates to a heterotrophic nitrification-aerobic denitrification composite bacterial agent capable of removing high ammonia nitrogen at low temperature and its application. The composite bacterial agent includes copper-lion bacteria SWA1, Alcaligenes faecalis, Acinetobacter and Paleobacterium TAC-2 composite It is formed, wherein the ratios of Copper greedy bacteria SWA1, Alcaligenes faecalis, Acinetobacter and Paleobacterium TAC‑2 are 10-20%: 5-20%: 10-30%: 20-50%. The composite bacterial agent of the present invention has the characteristics of high ammonia nitrogen resistance, low temperature resistance, heavy metal resistance, and synchronous nitrification and denitrification function, and can realize efficient denitrification of high ammonia nitrogen wastewater in an aerobic environment under low temperature conditions through the synergistic action of four microorganisms.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a heterotrophic nitrification-aerobic denitrification composite bacterial agent capable of removing high ammonia nitrogen at low temperature and its application. Background technique [0002] In recent years, the discharge of high ammonia nitrogen wastewater (ammonia nitrogen concentration > 200 mg / L) has been increasing. Improper treatment of high-ammonia-nitrogen wastewater and direct discharge into water bodies will lead to increasingly serious phenomena such as eutrophication of water bodies, resulting in deterioration of water body quality. In order to control ammonia nitrogen pollution in water bodies, my country has promulgated strict new standards for pollutant control, among which the primary standard for ammonia nitrogen is 5.0 mg / L. The new standard puts forward new requirements for the effective control of ammonia nitrogen, especially the effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C12R1/05
CPCC12N1/20C02F3/02C02F3/34Y02W10/10
Inventor 赵天涛艾铄何继杰张浩肖芃颖张晓凤
Owner CHONGQING UNIV OF TECH
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