Living cell bulk transferring plate and fabrication method thereof

Abstract

The invention provides a living cell bulk transferring plate and a fabrication method thereof, and relates to cell cytobiology and hydrobiology. The living cell bulk transferring plate is provided with a stainless steel sheet and a glass sheet, wherein the stainless steel sheet is vertically overlapped on the glass sheet and the stainless steel sheet and the glass sheet are pasted. The fabricationmethod comprises the steps of selecting the stainless steel sheet, selecting a laser cutting machine with a computer numerical control device, copying cutting data to a computer, performing laser oxygen cutting, cleaning burrs after cutting is completed, and polishing a cut; horizontally placing a quartz glass sheet on a transmitting plate, wherein a front surface and a back surface of an A-typestainless steel sheet are not distinguished, and a square working region scribing surface of a B-type stainless steel sheet is placed upwards; and coating AB glue onto the stainless steel sheet afterthe AB glue is uniformly mixed, vertically overlapping and placing the stainless steel sheet on the glass sheet, performing extrusion and alignment, scrapping residual glue of a side surface after theAB glue is completely cured, cleaning glue overflowing out of a round hole and a square working region due to extrusion, polishing the stainless steel piece, burnishing, cleaning powder, and fabricating the living cell bulk transferring plate.

Description

technical field [0001] The present invention relates to cell biology and aquatic biology, especially to the field of plankton biology, such as: plankton screening, purification culture, species identification, biological bait breeding, energy algae screening, sediment encapsulation research, etc., specifically related to living Cell batch transfer plates and methods for their preparation. Background technique [0002] In the study of plankton, scientific researchers often involve the screening, cleaning, transfer and cell image acquisition of living cells. Among them, during the preliminary screening of living cells, impurities (sediment, sediment, Debris, relatively small individual non-target cells and bacteria, etc.), it is necessary to wash the cells to keep the cells relatively pure, usually choose the water drop method, that is, drop a few drops of sterile water on the glass slide for use, in the microscope field of view Next, use a capillary to absorb cells from the ...

AI Technical Summary

Problems solved by technology

Water drop method operation, the volume of the water drop can be determined by the pipette, but the contact area between the water drop and the glass slide and the thickness of the water drop are affected by factors such as the material of the slide, cleanliness level, and even the degree of dryness, which are usually uncontrollable

In addition, some cells will continue to swim in the water droplet, even swim to the edge of the water droplet, and stay still, which is extremely difficult to find under the microscope and is easy to lose

At the same time, when the image is collected, the cell image sometimes appears deformed and distorted

In addition, glass slides generally do not need to be sterilized and are used directly, which is easy to bring in foreign bacteria, which increases the difficulty of cell culture

All these problems have caused some troubles to plankton researchers, especially phytoplankton researchers.

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