Copper metal organic framework mimic enzyme material as well as preparation and application thereof

An organic skeleton and enzyme-mimicking technology, applied in the field of enzyme-mimicking, can solve problems such as impeding the popularization and application of natural enzymes, difficulty in the application of natural enzymes, and high cost, and achieve excellent catalytic effects, intuitive and obvious effects, and low cost

Active Publication Date: 2018-12-28
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural enzymes also have their limitations
Natural enzymes are produced by living cells, so their development must be extracted and purified, but the extraction and purification of natural enzymes is not only difficult but also expensive, which greatly hinders the popularizatio...

Method used

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  • Copper metal organic framework mimic enzyme material as well as preparation and application thereof
  • Copper metal organic framework mimic enzyme material as well as preparation and application thereof
  • Copper metal organic framework mimic enzyme material as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of sheet-like Cu-MOFs mimic enzyme material

[0028] 1) Add 0.2mmol copper nitrate to 0.5mL N, N-dimethylformamide, and obtain 0.4mol / L solution A after magnetic stirring; add 0.2mmol 5-nitroisophthalic acid to 0.5mL N , in N-dimethylformamide, after magnetic stirring, 0.4mol / L solution B was obtained; solution A and 4mL methanol were added to solution B under stirring conditions, stirred for 20-40 minutes and then placed in a reaction kettle, At 75°C, react at a constant temperature for 48 hours; after the reaction is completed and cooled to room temperature, centrifuge and wash at a speed of 5000rpm, and then dry at 60°C for 24 hours to obtain a sheet-like Cu-MOFs mimic enzyme material (see figure 1 ). 4 mg of this material was dispersed into 20 mL of deionized water to obtain a 0.2 mg / mL Cu-MOFs mimic enzyme aqueous solution.

Embodiment 2

[0029] Example 2 Preparation of sheet Cu-MOFs mimic enzyme material

[0030] The difference from Example 1 is that the amounts of copper nitrate and 5-nitroisophthalic acid are both changed to 0.6 mmol. Add 0.6mmol copper nitrate to 0.5mL N,N-dimethylformamide, and obtain 1.2mol / L solution A after magnetic stirring; add 0.6mmol 5-nitroisophthalic acid to 0.5mL N,N - In dimethylformamide, after magnetic stirring, a solution B of 1.2 mol / L was obtained; solution A and 4 mL of methanol were added to solution B under stirring, and placed in a reaction kettle after stirring for 20 to 40 minutes. At 75°C, react at a constant temperature for 48 hours; after the reaction is completed and cooled to room temperature, centrifuge and wash at a speed of 5000 rpm, and then dry at 60°C for 24 hours to obtain a sheet-like Cu-MOFs simulation material, whose shape and size are the same as Embodiment 1 is consistent.

Embodiment 3

[0031] Example 3 To investigate the catalytic activity of the Cu-MOFs mimic enzyme material as a peroxide mimic enzyme, the following operations were performed:

[0032]Add 200 μL 0.2 mg / mL Cu-MOFs simulated enzyme solution, 200 μL pH=7.0 MOPS buffer solution, 200 μL 5 mM 3,3′,5,5′-tetramethylbenzidine, 80 μL 100 mM H 2 o 2 Solution and deionized water, so that the reaction system was 2mL, and solution C was obtained; 200μL buffer solution with pH=7.0, 200μL 5mM 3,3′,5,5′-tetramethylbenzidine solution, 80μL 100mM H 2 o 2 Solution and deionized water, so that the reaction system is 2mL, to obtain solution D; after reacting at 20°C for 5min, use a UV-visible spectrophotometer to detect the absorption spectra of solutions C and D respectively at 500-800 (see figure 2 ).

[0033] attached figure 2 It is shown that the sheet-like Cu-MOFs mimic enzyme material has peroxide mimic enzyme activity and has good catalytic performance.

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Abstract

The invention discloses a copper metal organic framework (Cu-MOFs) mimic enzyme material as well as preparation and application thereof. The nanotopography of the copper metal organic framework mimicenzyme material is in a flake shape. The Cu-MOFs mimic enzyme material can be prepared by the following steps: dissolving cupric nitrate into N,N-dimethylformamide to obtain a solution A and dissolving 5-nitroisophthalic acid into the N,N-dimethylformamide to obtain a solution B; adding the solution A and methanol into the solution B under the stirring condition, stirring for 20 to 40 minutes, putting a stirred solution in a reactor, and carrying out thermostatic reaction at the temperature of 70 to 80 DEG C for 48 to 72 hours; after the reaction is ended, centrifugally separating, washing, and then drying at 60 DEG C for 12 to 24 hours to obtain a flaky Cu-MOFs mimic enzyme material; dispersing the flaky Cu-MOFs mimic enzyme material into water to obtain a Cu-MOFs mimic enzyme solution. The material has excellent activity of mimetic peroxidase; compared with natural horse radish peroxidase (HRP), the material has the advantages of simple preparation process, low cost, good adaptability and the like; the material can be used as a substitute of the HRP, and has potential application value in aspects of bioinstrumentation, immunoassay and clinical diagnosis.

Description

technical field [0001] The invention relates to the field of simulated enzymes, in particular to a copper metal organic framework simulated enzyme material and its preparation method and application. Background technique [0002] Natural enzymes are macromolecular substances widely present in organism cells, most of which are composed of proteins (a few are RNA). Natural enzymes have the advantages of high catalytic efficiency and specificity, play a vital role in the metabolic process of living organisms, and participate in vital activities such as respiration, digestion, and exercise, so they have attracted extensive attention from researchers. However, natural enzymes also have their limitations. Natural enzymes are produced by living cells, so their development must be extracted and purified, but the extraction and purification of natural enzymes is not only difficult but also expensive, which greatly hinders the popularization and application of natural enzymes . At ...

Claims

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Application Information

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IPC IPC(8): B01J31/22B01J37/10B82Y40/00G01N21/31
CPCB01J31/1691B01J31/2213B01J37/10B82Y40/00G01N21/31
Inventor 汪嘉炎李文英郑岳青
Owner NINGBO UNIV
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