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A kind of ring theileriasis vaccine protective agent and preparation method

A vaccine protective agent and a Theilaria disease technology, which is applied in the field of annular Theileriasis vaccine protective agent and its preparation, can solve the problems of short shelf life, influence on vaccine transportation and use, and inability to protect cells, and achieve the effect of prolonging shelf life.

Active Publication Date: 2021-12-10
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing vaccine adopts the jelly vaccine protective agent developed in the 1970s, which was developed by Lanzhou Veterinary Research Institute. The protective agent protects the cells by adding lactalbumin hydrolyzate and sucrose as the main protective agent components. After 50 days of preservation, the cell viability drops to 33.5%, can not play a very good protective effect on cells
Studies have shown that although the vaccine prepared from the schizont stage of Theileria annuli can well control the spread and prevalence of this disease, the vaccine must be stored in liquid nitrogen or dry ice, and the storage period at 4 ° C is short and serious. Affected the transportation and use of the vaccine, as well as the application and promotion of the vaccine

Method used

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  • A kind of ring theileriasis vaccine protective agent and preparation method
  • A kind of ring theileriasis vaccine protective agent and preparation method
  • A kind of ring theileriasis vaccine protective agent and preparation method

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Experimental program
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Effect test

Embodiment Construction

[0018] 1. Basic method

[0019] 1. Materials and methods

[0020] 1.1 Cell source The cells used were schizont-infected lymphocytes of Theileria annulus Kashi strain isolated and cultured in the Laboratory of External Parasites and Vector-Borne Diseases of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, and were stably subcultured to F20 in vitro.

[0021] 1.2 Reagents Edible gelatin, industrial gelatin, fish scale gelatin, sodium selenite and zinc sulfate are domestic reagents; PBS buffer, citric acid buffer and HEPES buffer are self-prepared solutions; deionized water is produced by the laboratory itself; , RPMI-1640 was purchased from Biological Industries.

[0022] 1.3 Instruments and equipment Biological clean bench (Sujing Antai Air Technology Co., Ltd.); constant temperature incubator (Thermo); cell analyzer IC1000 (Countstar); inverted microscope (OLYMPUS IX71); pipette (Eppendorf).

[0023] 1.4 Method

[0024] 1.4.1 Identification o...

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Abstract

The invention discloses a vaccine protectant against ring-shaped theileriasis and a preparation method thereof. The ring-shaped Theileria vaccine protectant of the present invention is composed of: 2% edible gelatin, 0.01% sodium selenite, 0.01% zinc sulfate and 1% double antibody, and the buffer system is 0.01M PBS (PH7.2-7.4) and RPMI ‑1640. The ring-shaped theileria vaccine protectant of the present invention can prolong the storage period of the vaccine during transportation in a cold-chain environment at 4°C. After the cells are stored for 90 days, the cell viability is maintained at more than 89%, and the cells have the ability to re-grow. The result of the invention prolongs the shelf life of the vaccine, and provides strong technical support for the immune control of theileriosis annulare.

Description

technical field [0001] The invention relates to a protective agent for microorganisms, in particular to a protective agent for annular Theileriasis vaccine and a preparation method thereof. Background technique [0002] Bovine annular Theileriasis is a tick-borne blood protozoan disease. The pathogen of the disease is parasitic in the lymphocytes and red blood cells of cattle. The main clinical manifestations are high fever, anemia, jaundice and swelling of lymph nodes on the body surface. The incidence of the disease is And the high case fatality rate has caused huge economic losses to animal husbandry production. [0003] After Theileria annulus infects bovine lymphocytes, it forms schizonts in the cells. When the schizont-infected host cells are cultured in vitro, they can obtain the unlimited proliferation ability similar to tumor-like cells, and the parasites can be induced by subculture. weak. It has been confirmed that the parasites in the schizont stage have strong...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/02A61K39/002A61P33/02
CPCA61K39/002A61K47/02A61K47/42A61P33/02
Inventor 马全英关贵全刘学荣李有全刘军龙刘爱红李菁刘兆弼殷宏罗建勋
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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