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Green extraction method for increasing yield of dihydromyricetin

An extraction method and technology of dihydropoplar, applied in the direction of organic chemistry, etc., can solve the problems of many impurities in the extract and low extraction rate, and achieve the effects of high extraction rate, high extraction rate and purity, and reasonable design.

Inactive Publication Date: 2019-01-11
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method overcomes the problems of low extraction rate and more impurities in the extract in the previous methods. This method has the advantages of high efficiency, simplicity, environmental protection, low cost and suitable for certain scale production.

Method used

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  • Green extraction method for increasing yield of dihydromyricetin
  • Green extraction method for increasing yield of dihydromyricetin
  • Green extraction method for increasing yield of dihydromyricetin

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Experimental program
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Effect test

preparation example Construction

[0021] The preparation method of the vine tea powder is as follows: the raw material of the vine tea leaves is first dehydrated and dried until the moisture content is below 10%, crushed by a pulverizer through a sieve with a mesh size of 20 mesh or more, and the obtained powder is sealed and stored in a dry environment.

[0022] The liquid chromatographic conditions for the analysis of dihydromyricetin in the extract of vine tea were used in the experiment: using octadecylsilane bonded silica gel (C18) chromatographic column (Shimadzu WondaCract ODS-2 4.6mm×250mm, 5μm) as the filler , with methanol-0.1% formic acid (30:70) as the mobile phase; the detection wavelength is 292nm. The flow rate was 1.0 mL / min, the column temperature was 35°C, and the injection volume was 10 μl.

Embodiment 1

[0024] Take 0.4g of rattan tea powder and add 10ml of 3% β-cyclodextrin aqueous solution, let it stand for 10 minutes, ultrasonic power is 300W, after ultrasonic extraction for 8 minutes, centrifuge at 5000g for 10 minutes, absorb the supernatant to obtain the extract.

[0025] The resulting supernatant was concentrated and evaporated to dryness with a rotary evaporator under reduced pressure to obtain dihydromyricetin. figure 1 It is the HPLC spectrum of the dihydromyricetin reference substance, and the chromatographic peak with a retention time of 10.5 minutes is dihydromyricetin. figure 2 It is the HPLC collection of illustrative plates of the vine tea extraction sample of embodiment 1 of the present invention. The extraction yield of dihydromyricetin was 36.82%, and the purity was 89.94%.

Embodiment 2

[0027] Take 0.4g of rattan tea powder and add 10ml of 2% β-cyclodextrin aqueous solution, let it stand for 10 minutes, ultrasonic power is 420W, after ultrasonic extraction for 8 minutes, centrifuge at 5000g for 10 minutes, and absorb the supernatant to obtain the extract.

[0028] The resulting supernatant was concentrated and evaporated to dryness with a rotary evaporator under reduced pressure to obtain dihydromyricetin. The extraction yield of dihydromyricetin was 34.83%, and the purity was 87.11%.

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Abstract

A green extraction method for increasing yield of dihydromyricetin belongs to the technical field of extraction and separation of phytochemical components. The method comprises the following steps: (1) grinding: crushing dried Ampelopsis grossedentata leaves, and sieving the crushed leaves by a 40 mesh sieve in order to obtain Ampelopsis grossedentata powder; (2) extracting: mixing the Ampelopsisgrossedentata powder with an aqueous solution, adding beta-cyclodextrin, performing ultrasonic assisted extraction to obtain an extract, and centrifuging the extract or standing the extract overnightin order to obtain a supernatant and a solid material; and (3) concentrating: performing reduced pressure concentrating on the supernatant obtained in step (2) in order to obtain the dihydromyricetin.The method has the advantages of green and environmentally-friendly process, moderate cost and good promotion and application prospect.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of phytochemical components, and in particular relates to a green extraction method for increasing the yield of dihydromyricetin. Background technique [0002] Dihydromyricetin is a polyphenolic hydroxyl dihydroflavonol, which belongs to flavonoids. It mainly exists in the plant of Snake dentata (vine tea) plant. Studies have shown that dihydromyricetin has many pharmacological activities, such as anti-inflammatory, cough-relieving , expectorant, analgesic, antibacterial, antihypertensive, cellulite, cancer prevention, liver protection, etc. The chemical name of dihydromyricetin is 3, 5, 7, 3', 4', 5'-hexahydroxy-2, 3 dihydroflavonol, the English name is Dihydromyricetin, and the molecular formula is C 15 h 12 o 8 , the molecular weight is 320.25, and the structural formula is as follows: [0003] [0004] There are also other patented methods for extracting and separating...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 王丽玲王衍彬秦玉川柏明娥刘本同方茹
Owner ZHEJIANG FORESTRY ACAD