Method for preparing high-purity N-acetylneuraminic acid hydrate by supersaturation crystallization process

An acetylneuraminic acid and high-purity technology, which is applied in the field of preparing high-purity N-acetylneuraminic acid hydrate, can solve the problems of high price, difficulty in purity, unfavorable application of N-acetylneuraminic acid, etc., and achieves good quality, Simple process and stable crystal effect

Active Publication Date: 2019-01-11
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

With the approval of N-acetylneuraminic acid derived from microbial fermentation as a new food raw material by the National Health and Family Planning Commission, the demand for N-acetylneuraminic acid is also increasing. High-purity N-acetylneuraminic acid The demand is also increasing, but the purity of N-acetylneuraminic acid prepared from microbial ferm

Method used

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  • Method for preparing high-purity N-acetylneuraminic acid hydrate by supersaturation crystallization process
  • Method for preparing high-purity N-acetylneuraminic acid hydrate by supersaturation crystallization process

Examples

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Embodiment 1

[0023] In this embodiment, the method for preparing high-purity N-acetylneuraminic acid dihydrate by supersaturated crystallization method is as follows:

[0024] 1. Preheat the pure water to 50°C, then add 70% N-acetylneuraminic acid to prepare a supersaturated aqueous solution of N-acetylneuraminic acid with a concentration of 250g / L;

[0025] 2. Filtrate the supersaturated aqueous solution of N-acetylneuraminic acid obtained in step 1 while it is hot, and the filter membrane is 0.22um to remove a small amount of impurities;

[0026] 3. The supersaturated aqueous solution of N-acetylneuraminic acid after filtration is naturally cooled, then left to crystallize at 4°C, and matured for 24 hours after the crystals are precipitated, and then the crystal mother liquor is separated, and the separated crystals are washed with absolute ethanol for two Once dried at 100°C, N-acetylneuraminic acid dihydrate can be obtained.

[0027] Detected by high performance liquid chromatography,...

Embodiment 2

[0029] In this example, the method for preparing high-purity N-acetylneuraminic acid hydrate by supersaturated crystallization method is as follows:

[0030] 1. Preheat pure water to 60°C, then add N-acetylneuraminic acid with a content of 85% to prepare a supersaturated aqueous solution of N-acetylneuraminic acid with a concentration of 300g / L;

[0031] 2. Filtrate the supersaturated aqueous solution of N-acetylneuraminic acid obtained in step 1 while it is hot, and the filter membrane is 0.45um to remove a small amount of impurities;

[0032] 3. The supersaturated aqueous solution of N-acetylneuraminic acid after filtration is cooled naturally, then left to crystallize at 10°C, and matured for 36 hours after the crystals are precipitated, and then the crystal mother liquor is separated, and the separated crystals are quickly washed with pure water for two Once dried at 80°C, N-acetylneuraminic acid dihydrate can be obtained.

[0033] Detected by high performance liquid chro...

Embodiment 3

[0035] This implementation follows the steps of Example 2, except that the aqueous solution concentration of N-acetylneuraminic acid in step 1 is 40g / l, other conditions are the same as the steps of Example 4, and finally there is no crystallization of N-acetylneuraminic acid dihydrate .

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Abstract

The invention discloses a method for preparing high-purity N-acetylneuraminic acid hydrate by a supersaturation crystallization process. The N-acetylneuraminic acid hydrate is prepared through low-temperature standing crystallization of a supersaturated aqueous solution of N-acetylneuraminic acid. A result of detection after drying of the N-acetylneuraminic acid hydrate shows that the purity is 99% or more, so requirements in the fields of foods, health care and medicines are met; and the method is simple, is easy to operate, and is especially suitable for industrial fermentation production ofthe N-acetylneuraminic acid hydrate.

Description

technical field [0001] The invention relates to a method for preparing high-purity N-acetylneuraminic acid hydrate by using a supersaturated crystallization method, belonging to the technical field of biochemical engineering. Background technique [0002] N-acetylneuraminic acid, also known as sialic acid and bird's nest acid, has the effects of improving intelligence, memory and anti-senile dementia. N-acetylneuraminic acid hydrate is N-acetylneuraminic acid with crystal water. At present, there is a lot of demand for N-acetylneuraminic acid dihydrate in the market, but its preparation technology and production capacity are key issues. Currently, acetylneuraminic acid The supply of neuraminic acid dihydrate is in short supply; in addition, the crystal structure of N-acetylneuraminic acid dihydrate is a single crystal structure, so the purity of the compound is high, and there is demand for applications with high purity requirements such as medicine and health food. With th...

Claims

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Application Information

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IPC IPC(8): C07H7/033C07H1/06
CPCC07H1/06C07H7/033
Inventor 袁丽霞陈祥松吴金勇孙立洁李翔宇朱薇薇王纪吴志波姚建铭
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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