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Method for obtaining chlorogenic acid by fermenting leaves of Acer truncatum

A technology of chlorogenic acid and ingot maple, applied in the field of obtaining chlorogenic acid by fermentation of ingot maple leaves, can solve the problems of high extraction temperature by water extraction, easy inactivation of chlorogenic acid, low enzyme activity efficiency, etc., and the fermentation conditions are easy to control. , easy to mass production, the effect of many metabolites

Inactive Publication Date: 2019-01-11
湖南中茂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the extraction of chlorogenic acid, the main raw materials currently used are eucommia leaves and honeysuckle. The main extraction methods are water extraction, organic solvent method and enzymatic hydrolysis method. The extraction temperature of water extraction method is high, the extraction rate is low, and chlorogenic acid is easy to lose activity ; Although the extraction efficiency of the organic solvent method is high, there are defects such as solvent residues; the enzymatic hydrolysis method has mild conditions and good product activity, but the price is expensive, which limits its application in large-scale production
The extraction of chlorogenic acid using maple ingots is currently limited to the above traditional methods. Although the cost of raw materials has been reduced, the demand for organic solvents or enzymes is large, and the problem of low enzyme activity efficiency still exists.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Take 200g of fresh maple ingot leaves and rinse them with tap water, then soak them in 70% ethanol for 30s, and finally rinse them with sterile water for 3 times; grind the cleaned leaf tissue with ultrasound (power 400w, 2min) to break the cell wall, and make maple ingots stock solution.

[0031] 2. Culture medium preparation: 1) Preparation of the fermented medium of Maple indica: 10 g of sucrose, 20 g of ammonium chloride, 0.02 g of copper sulfate and 200 g of maple ingot stock solution, add water to 1 L, and sterilize; 2) Activate the culture medium of the strain : 200 g fresh potatoes, peel the potatoes, cut into pieces, add appropriate amount of water and boil for 30 min; then filter the potato boiling liquid with 6 layers of gauze, add water to 1L; weigh 20 g of glucose, dissolve 20 g of agar in potato boiling water Liquid, pH natural, sterilized.

[0032] 3. After activating the Bacillus subtilis strain on the potato medium for 24 hours, adjust the concentra...

Embodiment 2

[0037] 1. Take 200g of fresh maple ingot leaves and rinse them with tap water, then soak them in 70% ethanol for 30s, and finally rinse them with sterile water for 3 times; grind the cleaned leaf tissue with ultrasound (power 400w, 2min) to break the cell wall, and make maple ingots stock solution;

[0038] 2. Culture medium preparation: 1) Preparation of the fermented medium of Maple indica: 15g of sucrose, 10 g of ammonium chloride, 0.01 g of copper sulfate and 300 g of maple ingot stock solution, add water to 1L, and sterilize; 2) Strain activation medium: 200 g fresh potatoes, peel the potatoes, cut into pieces, add appropriate amount of water and boil for 30 minutes; then filter the potato boiling liquid with 6 layers of gauze, add water to 1L; weigh 20g of glucose, 20g of agar and dissolve in the potato boiling liquid , pH natural, sterilized.

[0039] 3. After activating the Bacillus subtilis strain on the potato medium for 24 hours, adjust the concentration of the bac...

Embodiment 3

[0044] 1. Take 200g of fresh maple ingot leaves and rinse them with tap water, then soak them in 70% ethanol for 30s, and finally rinse them with sterile water for 3 times; grind the cleaned leaf tissue with ultrasound (power 400w, 2min) to break the cell wall, and make maple ingots stock solution.

[0045] 2. Culture medium preparation: 1) Preparation of the fermented medium of Maple indica: 20 g of sucrose, 10 g of ammonium chloride, 0.01 g of copper sulfate and 400 g of maple ingot stock solution, add water to 1 L, and sterilize; 2) Strain activation medium : 200 g fresh potatoes, peel the potatoes, cut into pieces, add appropriate amount of water and boil for 30 min; then filter the potato boiling liquid with 6 layers of gauze, add water to 1L; weigh 20 g of glucose, dissolve 20 g of agar in potato boiling water Liquid, pH natural, sterilized.

[0046] 3. After activating the Bacillus subtilis strain on the potato medium for 24 hours, adjust the concentration of the bacte...

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Abstract

The invention provides a method for obtaining chlorogenic acid by fermenting leaves of Acer truncatum. The method uses fresh leaves of Acer truncatum as raw materials and utilizes microorganisms suchas Bacillus to ferment the extract of leaves of Acer truncatum, and fully utilizes chlorogenic acid precursor substance in leaves of Acer truncatum to carry out biological transformation in the fermentation process, thereby accumulating chlorogenic acid in large quantities. The method comprises the step: extracting Acer truncatum leaves by breaking walls, preparing fermentation culture medium, inoculating the strain into fermentation culture medium for culture, and centrifuging the supernatant after fermentation to obtain chlorogenic acid for separation and purification. The fermentation culture medium is composed of 10-20g of sucrose, 10-20g of ammonium chloride, 0. 01-0.02g of cop sulfate, 200-400g of maple syrup and 1000mL of water. The method has the advantages of low cost, high yield,high purity, good activity, simple operating and being suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of chlorogenic acid preparation, and more specifically relates to a method for obtaining chlorogenic acid by fermenting ingot maple leaves. Background technique [0002] Yuanbao Maple is a deciduous tree of the genus Acer, which is a unique tree species in my country. It is widely planted in China and has abundant resources. Yuanbao maple leaf is rich in a variety of biologically active ingredients, such as flavonoids, chlorogenic acid, cardiac glycosides, steroids, tannins, polysaccharides, etc., and the content of flavonoids and chlorogenic acid is relatively high, and it is also rich in vitamins and mineral elements , 9 kinds of amino acids necessary for the human body, SOD and catechin, etc. Chlorogenic acid has the functions of inhibiting hyaluronidase and glucose-6-phosphatase, scavenging free radicals, resisting lipid peroxidation, antimutagenic, antibacterial, antiviral and protecting liver and chol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/125
CPCC12P7/62
Inventor 赵勇彪李佳莲危志刚赵勇志
Owner 湖南中茂生物科技有限公司
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