A method for detecting ancient silk fabrics based on electrochemiluminescence

A luminescence and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc. It can solve the problems of large interference of impurities, low sensitivity, and unsuitability for cultural relics detection. , to achieve the effect of increasing sensitivity, improving sensitivity, and excellent catalytic performance

Active Publication Date: 2021-04-20
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structures and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. It has a large impact and is not suitable for the detection of cultural relics. Therefore, it is necessary to develop a method for the detection of silk fabrics with good sensitivity and specificity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]A) Weigh 20 mg of cadmium / zinc sulfide quantum dots, 118 g of polymethyl methacrylate, 78 mg of polymarine-eotellic polymene copolymers, add 1.8 ml of chloroform, with 4.5 ml of 3 mg / ml After mixing the sodium dodecyl sulfonate solution, the chloroform was evaporated after mixing with an ultrasonic homogenizer. The resulting water-soluble quantum dot bead was then purified to wash them twice with deionized water;

[0035]B) Take 0.1 mg of the medium quantum dies in step a), add 20 μl of 1- (3-dimethylaminopropyl) -3-ethyl carbodiimide hydrochloride, N-hydroxy succinimide, reaction 2h;

[0036]C) Take 4 mL of 0.2 mg / ml of the graphene solution and 180 μl of 1 mm hexametopolymented potassium solution solution, reacts in an ice water bath, and then 0.8 mg of sodium borohydride is added after 1 h, after vigorous stirring. Centrifuge, wash twice using deionized water, dry.

[0037]D) Add platinum-containing oxide in step c), 0.3 ml of dimethylformamide, ultrasonic treatment for 2 h, t...

Embodiment 2

[0047]A) Weigh 20 mg of cadmium / zinc sulfide quantum dots, 120 mg of polymethyl methacrylate, 80 mg of polymarine-eotellic polymene copolymer, add 2 mL of chloroform, twelve of 5 ml of 3 mg / ml The sodium alkyl sulfonate is mixed with an ultrasonic homogenizer, and then the chloroform is evaporated. The resulting water-soluble quantum dot bead was then purified to clean 3 times with deionized water;

[0048]B) Taking step a) 0.12 mg of medium quantum dies, 20 μl of 1- (3-dimethylaminopropyl) -3-ethyl carbon diimide hydrochloride, N-hydroxy succinimide, reaction 2H;

[0049]C) 4 mL of 0.25 mg / ml of the graphene solution and 200 μl of 1 mm 1mm hexapipoperate solution, reacted in an ice water bath, and then 1 mg of sodium borohydride was added to 1 h after stirring, centrifuged. Wash 3 times with deionized water, dry.

[0050]D) Add 0.4 mg of step c) containing platinum oxide oxide, 0.4 ml of dimethylformamide, ultrasonic treatment for 2 h, then add 0.05 g of propylene metamine and 0.01 g ...

Embodiment 3

[0060]A) Weigh 20 mg of cadmium / zinc sulfide quantum dots, 122 mg of polymethyl methacrylate, 82 mg of polymaric anhydride-eotellic copolymer, add 2.2 ml of chloroform, 5.5 ml of 3 mg / ml After mixing the sodium dodecyl sulfonate solution, the chloroform was evaporated after mixing with an ultrasonic homogenizer. The resulting water-soluble quantum dot bead was then purified, and 4 times was cleaned with deionized water;

[0061]B) Take 0.14 mg of the medium quantum dies in step a), add 20 μl of 1- (3-dimethylaminopropyl) -3-ethyl carbodiimide hydrochloride, N-hydroxy succinimide, reaction 2H;

[0062]C) Take 4 mL of 0.3 mg / ml of the oxide solution with 220 μl of 1 mm 1mm hexametoplatin platinite solution, reacts in an ice water bath, and then add 1.2 mg of sodium borohydride to 1 h after stirring, centrifugation , Wash 4 times with deionized water, dry.

[0063]D) Add platinum-containing oxide in step c), 0.5 ml of dimethylformamide, ultrasonic treatment for 2 h, then add 0.06 g of pro...

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PUM

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Abstract

The invention relates to the field of cultural relic detection, and discloses a method for detecting ancient silk fabrics based on electrochemiluminescence. The invention first prepares cadmium selenide / zinc sulfide quantum dot beads, marks them on platinum-modified graphene oxide, and then Adsorb the silk fibroin antibody to form a probe, and then incubate with the cultural relic sample modified on the working electrode for a period of time, and the species of the cultural relic sample can be judged according to the fluorescent signal obtained under electrochemical scanning. When the present invention detects ancient silk fabrics, It has the characteristics of less sample consumption, intuition, accuracy and high sensitivity.

Description

Technical field[0001]The present invention relates to the field of cultural retriever detection, and more particularly to a method of detecting ancient wire fabric based on electrochemical illumination.Background technique[0002]China since ancient times, China is the textile big country, the textile is rich in textiles, beautiful craftsmanship, comfortable and breathable. The most famous textile is China's silk, so China is called "Silk Country." The main ingredients of silk are silkworms, and silk is mainly composed of three parts: silk fibroin and silk gel. The silk fibroin is the main component of silk, accounting for 70% of total weight. And silk is used as an organic polymer material, which is susceptible to light, hot, acid base, microbial, and the like, resulting in a change in structural and performance of crystallinity, molecular weight, etc., so conventional detection method has low sensitivity, and impurities interference Big, not suitable for testing of artifacts, theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/327G01N27/30
CPCG01N21/76G01N27/308G01N27/3275
Inventor 王秉李青青欧阳毅刘林帅彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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