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Method for detecting ancient silk fabric based on electrochemiluminescence

A luminescence method and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions. It can solve the problems of large interference of impurities, low sensitivity, and unsuitability for cultural relics detection. , to achieve the effects of accurate sensitivity, improved sensitivity, and good biocompatibility

Active Publication Date: 2019-01-11
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structures and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. It has a large impact and is not suitable for the detection of cultural relics. Therefore, it is necessary to develop a method for the detection of silk fabrics with good sensitivity and specificity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 118g of polymethyl methacrylate, 78mg of polymaleic anhydride-octadecene copolymer, add 1.8ml of chloroform, and 4.5ml of 3mg / ml The aqueous sodium dodecylsulfonate solution was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified, and washed twice with deionized water;

[0035] B) Weigh 0.1 mg of quantum dot beads in step A), add 20 µl of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide, and react 2h;

[0036] C) Mix 4ml of 0.2mg / ml graphene oxide solution with 180µl of 1mM potassium hexachloroplatinate solution, react in an ice-water bath, and then add 0.8mg of sodium borohydride to react for 1h under vigorous stirring. Centrifuge, wash twice with deionized water, and dry.

[0037] D) Take 0.3-0.5mg of platinum-containing graphene oxide in step C), add 0....

Embodiment 2

[0047] A) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 120mg of polymethyl methacrylate, 80mg of polymaleic anhydride-octadecene copolymer, add in 2ml of chloroform, and mix with 5ml of 3mg / ml dodecyl The aqueous sodium alkylsulfonate solution was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified, and washed 3 times with deionized water;

[0048] B) Weigh 0.12 mg of quantum dot beads in step A), add 20 µl of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide, and react 2h;

[0049] C) Mix 4ml of 0.25 mg / ml graphene oxide solution with 200µl of 1mM potassium hexachloroplatinate solution, react in an ice-water bath, then add 1mg of sodium borohydride to react for 1h under vigorous stirring, and then centrifuge. Wash 3 times with deionized water and dry.

[0050] D) Take 0.4 mg of platinum-containing graphene oxi...

Embodiment 3

[0060] A) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 122mg of polymethyl methacrylate, 82mg of polymaleic anhydride-octadecene copolymer, add 2.2ml of chloroform, and 5.5ml of 3mg / ml The aqueous sodium dodecylsulfonate solution was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified, and washed 4 times with deionized water;

[0061] B) Weigh 0.14 mg of quantum dot beads in step A), add 20 µl of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide, and react 2h;

[0062] C) Mix 4ml of 0.3 mg / ml graphene oxide solution with 220µl of 1mM potassium hexachloroplatinate solution, react in an ice-water bath, then add 1.2mg of sodium borohydride to react for 1h under vigorous stirring, then centrifuge , washed 4 times with deionized water, and dried.

[0063] D) Take 0.3-0.5mg of platinum-containing graphene oxide in st...

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PUM

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Abstract

The invention relates to the field of cultural relic detection and discloses a method for detecting an ancient silk fabric based on electrochemiluminescence. The method of the invention comprises thefollowing steps: preparing cadmium selenide / zinc sulfide quantum dot beads, marking platinum-modified graphene oxide with the cadmium selenide / zinc sulfide quantum dot beads, then allowing the beads to be adsorbed onto a silk fibroin antibody to form a probe, subjecting the probe and a cultural relic sample which is modified on a working electrode to incubation for a period of time, and finally determining the species of the cultural relic sample according to fluorescent signals obtained through electrochemical scanning. The method of the invention has the characteristics of low sample usage amount, visuality, accuracy and high sensitivity when applied to detection of the ancient silk fabric.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for detecting ancient silk fabrics based on an electrochemiluminescence method. Background technique [0002] China has been a big country of textiles since ancient times. The textiles produced are rich in variety, exquisite in craftsmanship, comfortable and breathable. Among them, the most famous textile is Chinese silk, so China is also known as the "Country of Silk". The main component of silk is mulberry silk, which is mainly composed of silk fibroin and sericin. Silk fibroin is the main component of silk, accounting for about 70% of the total weight. And as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structure and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. ...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/327G01N27/30
CPCG01N21/76G01N27/308G01N27/3275
Inventor 王秉李青青欧阳毅刘林帅彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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