Method for detecting ancient silk fabric based on microwave

A technology for microwave detection and silk fabrics, which is applied in the field of cultural relics detection, can solve the problems of high impurity interference, low sensitivity, and unsuitability for cultural relics detection, and achieve high sensitivity, increased sensitivity, and good detection effect

Active Publication Date: 2019-01-11
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structures and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. It has a large impact and is not suitable for the detection of cultural relics. Therefore, it is necessary to develop a method for the detection of silk fabrics with good sensitivity and specificity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A) Weigh 5g of mulberry silk and place it in 180mL of 0.018M sodium carbonate solution, bathe in water for 55min at a temperature of 80°C, take it out and wash it with deionized water for more than three times, and put it in an oven to dry.

[0033] B) Take 2g of dried silk fibroin, 2.3g of calcium nitrate, add 48mL of formic acid, stir with magnetic force for 80min, filter and add sodium bicarbonate until the solution is neutral, and use a cellulose dialysis bag with a molecular weight cut-off of 8000 to dialyze in deionized water for 2 day, and change the water every 5 hours, freeze-dry the silk fibroin solution in a vacuum freeze dryer for 2 days, and grind the obtained silk fibroin into powder for later use.

[0034] C) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 118mg of polymethyl methacrylate, 78mg of polymaleic anhydride-octadecene copolymer, add in 1.8ml of chloroform, mix with 4.5ml of 3mg / ml The aqueous sodium dodecylsulfonate solution was mixed...

Embodiment 2

[0043] A) Weigh 5g of mulberry silk and place it in 200mL of 0.02M sodium carbonate solution, bathe in water for 60min at a temperature of 80°C, take it out and wash it with deionized water for more than three times, and put it in an oven to dry.

[0044] B) Take 2 g of dried silk fibroin, 2.5 g of calcium nitrate, add 50 mL of formic acid, stir with magnetic force for 90 min, filter and add sodium bicarbonate until the solution is neutral, dialyze in deionized water for 2.5 g with a cellulose dialysis bag with a molecular weight cut-off of 9000 day, and change the water every 6 hours, freeze-dry the silk fibroin solution in a vacuum freeze dryer for 2.5 days, and grind the obtained silk fibroin into powder for later use.

[0045] C) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 120mg of polymethyl methacrylate, 80mg of polymaleic anhydride-octadecene copolymer, add in 2ml of chloroform, and mix with 5ml of 3mg / ml dodecyl The aqueous sodium alkylsulfonate solution ...

Embodiment 3

[0054] A) Weigh 5g of mulberry silk and place it in 220mL of 0.022M sodium carbonate solution, bathe in water for 65min, the temperature of the water bath is 80°C, take it out and wash it with deionized water for more than three times, and put it in an oven to dry;

[0055] B) Take 2 g of dried silk fibroin, 2.7 g of calcium nitrate, add 52 mL of formic acid, stir with magnetic force for 100 min, filter and add sodium bicarbonate until the solution is neutral, and use a cellulose dialysis bag with a molecular weight cut-off of 10,000 to dialyze in deionized water for 3 day, and change the water every 7 hours, freeze-dry the silk fibroin solution in a vacuum freeze dryer for 3 days, and grind the obtained silk fibroin into powder for later use;

[0056] C) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 122mg of polymethyl methacrylate, 82mg of polymaleic anhydride-octadecene copolymer, add 2.2ml of chloroform, and 5.5ml of 3mg / ml The aqueous sodium dodecylsulfonate s...

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PUM

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Abstract

The invention relates to the field of cultural relic detection, and discloses a method for detecting ancient silk fabric based on microwave. The invention first adopts a calcium nitrate and formic acid system to prepare silk fibroin protein, then prepares a quantum dot bead labeled silk fibroin antibody, and performs microwave Elisa detection on the silk fibroin and the silk fabric cultural relics, so that whether the cultural relics contain mulberry silk can be determined by the fluorescence intensity value. The method provided by the invention is low in sample dosage, has the advantages of fast speed, accuracy and high sensitivity, and has good detection effect on the spoiled silk fabric.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for detecting ancient silk fabrics based on microwaves. Background technique [0002] China has been a big country of textiles since ancient times. The textiles produced are rich in variety, exquisite in craftsmanship, comfortable and breathable. Among them, the most famous textile is Chinese silk, so China is also known as the "Country of Silk". The main component of silk is mulberry silk, which is mainly composed of silk fibroin and sericin. Silk fibroin is the main component of silk, accounting for about 70% of the total weight. And as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structure and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. It has a great influence ...

Claims

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Application Information

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IPC IPC(8): G01N33/36G01N33/58
CPCG01N33/365G01N33/581G01N33/582G01N33/588
Inventor 王秉李青青刘林帅欧阳毅彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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