Method for detecting ancient silk fabric based on immunochromatography technique

A technology of immunochromatography and silk fabrics, which is applied in the field of detection of ancient silk fabrics based on immunochromatography technology, can solve the problems of high impurity interference, unsuitability for cultural relics detection, low sensitivity, etc., achieve less sample consumption, reduce damage, The effect of increasing solubility

Inactive Publication Date: 2019-01-11
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structures and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. It has a large impact and is not suitable for the detection of cultural relics. Therefore, it is necessary to develop a fast, simple and sensitive method for detecting ancient silk fabrics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 118mg of polymethyl methacrylate, 78mg of polymaleic anhydride-octadecene copolymer, add 1.8ml of chloroform, and 4.5ml of 3mg / ml The aqueous sodium dodecylsulfonate solution was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified at a centrifugation rate of 8000 rpm for 8 min, and washed twice with deionized water.

[0026] B) Weigh 5g of mulberry silk and place it in 180mL of 0.018M sodium carbonate solution, bathe in water for 55min at a temperature of 80°C, take it out and wash it with deionized water for more than three times, and put it in an oven to dry.

[0027] C) Take 2g of dried silk fibroin, 2.3g of calcium nitrate, add 48mL of formic acid, stir with magnetic force for 80min, filter and add sodium bicarbonate until the solution is neutral, dialyze and freeze-dry, grind the obtain...

Embodiment 2

[0034] A) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 120mg of polymethyl methacrylate, 80mg of polymaleic anhydride-octadecene copolymer, add in 2ml of chloroform, and mix with 5ml of 3mg / ml dodecyl The aqueous sodium alkylsulfonate solution was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then the obtained water-soluble quantum dot beads were centrifuged and purified at a centrifugation rate of 10000 rpm for 10 min, and washed three times with deionized water.

[0035] B) Weigh 5g of mulberry silk and place it in 200mL of 0.02M sodium carbonate solution, bathe in water for 60min at a temperature of 80°C, take it out and wash it with deionized water for more than three times, and put it in an oven to dry.

[0036] C) Take 2g of dried silk fibroin, 2.5g of calcium nitrate, add 50mL of formic acid, stir with magnetic force for 90min, filter and add sodium bicarbonate until the solution is neutral, dialyze and freeze-...

Embodiment 3

[0043] A) Weigh 20mg of cadmium selenide / zinc sulfide quantum dots, 122mg of polymethyl methacrylate, and 82mg of polymaleic anhydride-octadecene copolymer, add 2.2ml of chloroform, and mix with 5.5ml of 3mg / ml The aqueous sodium dodecylsulfonate solution was mixed and treated with an ultrasonic homogenizer, after which the chloroform was evaporated. Then, the obtained water-soluble quantum dot beads were centrifuged and purified at a centrifugation rate of 12000 rpm for 12 min, and washed 4 times with deionized water.

[0044] B) Weigh 5g of mulberry silk and place it in 220mL of 0.022M sodium carbonate solution, bathe in water for 65min at a temperature of 80°C, take it out and wash it with deionized water for more than three times, and put it in an oven to dry.

[0045] C) Take 2g of dried silk fibroin, 2.7g of calcium nitrate, add 52mL of formic acid, stir with magnetic force for 100min, filter and add sodium bicarbonate until the solution is neutral, dialyze and freeze-dr...

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PUM

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Abstract

The invention relates to the field of cultural relic detection, and discloses a method for detecting an ancient silk fabric based on an immunochromatography technique. According to the invention, a formic acid-calcium nitrate solution system is adopted for extracting silk fibroin, and then a cadmium selenide/zinc sulfide quantum dot is used for preparing a highly-luminescent quantum dot bead. Whenimmunochromatographic test paper is used for detection, the quantum dot bead is bonded with a silk fibroin antibody; a T line is sprayed with silk fibroin, and a C line is sprayed with a goat anti-rabbit antibody; a bonded body of a to-be-detected cultural relic sample of a silk fabric and the quantum dot bead silk fibroin antibody moves under chromatographic action; and the species of the cultural relic sample can be determined based on the fluorescence conditions of the T and C lines. The method of the invention has the characteristics of rapidness, visuality, accuracy and high sensitivitywhen applied to detection of cultural relics of silk fabrics.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for detecting ancient silk fabrics based on immunochromatography technology. Background technique [0002] China has been a big country of textiles since ancient times. The textiles produced are rich in variety, exquisite in craftsmanship, comfortable and breathable. Among them, the most famous textile is Chinese silk, so China is also known as the "Country of Silk". The main component of silk is mulberry silk, which is mainly composed of silk fibroin and sericin. Silk fibroin is the main component of silk, accounting for about 70% of the total weight. And as an organic polymer material, mulberry silk is easily degraded by light, heat, acid-base, microorganisms, etc., resulting in changes in crystallinity, molecular weight and other structure and properties. Therefore, conventional detection methods have low sensitivity and are interfered by impurities. It h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58
CPCG01N33/558G01N33/582
Inventor 王秉李青青欧阳毅刘林帅彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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