A a preparation method of autogenous collagen gel with hyaluronic acid

A collagen and hyaluronic acid technology, applied in the field of medical cosmetology, can solve problems such as being easily degraded by the body and unable to fundamentally solve the problem of wrinkles

Inactive Publication Date: 2019-01-15
白晋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Removing wrinkles through autologous collagen injection can significantly reduce allergic reactions, but simple collagen injection is easily degraded by the body and cannot funda...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The embodiment of the present invention provides a preparation method of autologous collagen gel with hyaluronic acid, comprising the following steps:

[0021] Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it and mix, centrifuge at room temperature to remove the lower layer liquid, rinse with phosphate buffered saline solution Centrifuge again, the centrifugal speed is 1000rpm-2500rpm, the centrifugation time is 2min-5min, remove the supernatant, add collagenase, place it in a constant temperature shaking box at 37°C for 30min-60min, centrifuge again to remove the supernatant, and then add fetal bovine The DMEM culture fluid of serum, the content of fetal bovine serum in DMEM culture fluid is 15%, centrifuges to remove supernatant after filtering with cell sieve, adds in the DMEM culture fluid containing fetal bovine serum a...

Embodiment 1

[0032] Autologous collagen gel with hyaluronic acid was prepared using the following steps:

[0033]Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it and mix, centrifuge at room temperature to remove the lower layer liquid, rinse with phosphate buffered saline solution Centrifuge again, the centrifugal speed is 1000rpm, the centrifugation time is 5min, remove the supernatant, add collagenase, place in a constant temperature shaking box at 37°C for 60min, centrifuge again to remove the supernatant, and then add DMEM culture medium containing fetal bovine serum, The content of fetal bovine serum in the DMEM culture medium is 15%. After filtering with a cell sieve, the supernatant is removed by centrifugation, and then added to the DMEM culture medium containing fetal bovine serum. 2 Constant temperature culture;

[0034] Step 2, sub...

Embodiment 2

[0039] Autologous collagen gel with hyaluronic acid was prepared using the following steps:

[0040] Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it and mix, centrifuge at room temperature to remove the lower layer liquid, rinse with phosphate buffered saline solution Centrifuge again at a centrifugal speed of 1500rpm for 4min, remove the supernatant, add collagenase, place in a constant temperature shaking box at 37°C for 60min, centrifuge again to remove the supernatant, and then add DMEM culture medium containing fetal bovine serum, The content of fetal bovine serum in the DMEM culture medium is 15%, and the supernatant is removed by centrifugation after filtering with a cell sieve, and then added to the DMEM medium containing fetal bovine serum again, at 37°C, 5% CO 2 Constant temperature culture;

[0041] Step 2, subculture...

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Abstract

The invention relates to a preparation method of autogenous collagen gel with hyaluronic acid, belonging to the technical field of medical cosmetology, comprising the following steps: step 1, obtaining autogenous fibroblast tissue; step 2, subculturing; step 3, accumulating collagen; 4, adding hyaluronic acid; step 5: preparing gel. The technical scheme takes collagen produced by autologous fibroblasts as raw material. Hyaluronic acid and crosslink agent are mixed to prepare gel product, the autogenous collagen gel has no toxic and side effects. The autogenous collagen gel has good biocompatibility and histocompatibility, and can be used as tissue filler in medical cosmetology. Vascular endothelial cell growth factor can promote the healing and growth of subcutaneous blood vessel after filling, promote blood circulation, moisten skin, eliminate wrinkles and prevent color spots, and has good cosmetology effect.

Description

technical field [0001] The invention relates to a preparation method of autologous collagen gel with hyaluronic acid, belonging to the technical field of medical cosmetology. Background technique [0002] Wrinkles refer to the small fine lines and wrinkles formed by the skin being affected by the external environment to form free radicals, free radicals destroy collagen and active substances in normal cell membrane tissues, and oxidize cells. There are four reasons for the occurrence of wrinkles: natural aging, gravity, ultraviolet radiation in the sun, which causes photoaging and photodamage of the skin and excessive contraction of facial expression muscles. Among them, the wrinkles produced by excessive contraction of facial expression muscles are called dynamic wrinkles, mainly manifested as crow's feet on the outer side of the orbit, forehead lines and glabella lines, nasolabial folds, supralabial lines and platysma lines. There are many ways to remove wrinkles. Topical...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/24A61L27/20A61L27/54A61L27/22
CPCA61L27/20A61L27/227A61L27/24A61L27/50A61L27/54A61L2300/414A61L2400/06A61L2430/34C08L5/08
Inventor 白晋
Owner 白晋
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