A kind of C. inflata bacterial strain and application thereof

A technology of bacterial strains and spores, applied in the field of microorganisms, can solve the problems of insecticidal activity and insecticidal mechanism research that have not yet been reported, and achieve the effects of strong insecticidal activity, reduced dosage, and good insecticidal effect

Active Publication Date: 2020-12-04
CHONGQING TAIJI MEDICAL RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, domestic and foreign research on T.inflatum mainly focuses on the antibacterial activity of secondary metabolites and the production and stability of cyclosporin, and the research on its insecticidal activity and insecticidal mechanism is still in progress. no report

Method used

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  • A kind of C. inflata bacterial strain and application thereof
  • A kind of C. inflata bacterial strain and application thereof
  • A kind of C. inflata bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The isolation of embodiment 1 bacterial strain

[0044]Disinfect the surface of the collected naturally infected bat moth larvae with 70% alcohol, put them in a 25°C incubator and culture them for 3-5 days, so that fresh spores grow on the surface of the insects; under sterile conditions, pick them with an inoculation loop Take a small amount of fresh spores and streak them on the PDA medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 25°C incubator for cultivation; after 3-4 days, select the typical ones without bacteria Use a transfer needle to pick up a small piece of medium with hyphae at the edge of the colony, transfer it to the center of the culture medium on the slant of the test tube, and culture at a constant temperature of 25°C for 10 days.

Embodiment 2

[0045] The identification of embodiment 2 bacterial strains

[0046] (1) Morphological identification

[0047] The isolated (SCKDC10) strain was inoculated on a PDA plate and cultured at 25°C to observe the morphological characteristics of the colony, the sporulation structure, and the shape and size of the spores. see figure 2 , Inoculated on a PDA plate with the strain SCKDC10 of Curvularia magnum of the present invention, displayed on a PDA medium plate, cultivated at a constant temperature of 25°C, the strain was cultivated for 5 days, and the colony was round and white, fluffy, with a diameter of 2-4cm; the middle of about 7 days of culture Fluffy fluffy, radial fluffy edges, 5-8cm in diameter; 14 days later, the colony diameter is 10-12cm, the colony is still white, the edges are neat, the hyphae are evenly raised, the fluff becomes rare, the back is light yellow, and the back of the colony changes from the initial The yellow and white color gradually turns dark yello...

Embodiment 3

[0053] Example 3 Determination of Indoor Insecticidal Activity of SCKDC10 Strain to Lepidoptera Model Insect Bombyx mori 3rd Instar Larvae and Pupa

[0054] Take the SCKDC10 strain grown on the PDA medium for 7 days, scrape the conidia under aseptic conditions, suspend them in 0.02% Tween 80 sterile water, filter and collect the conidia, and configure 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 5 different concentrations of conidia / mL.

[0055] Take 60 silkworms from the 4th instar silkworm, set up 3 replicates for each strain, 20 heads for each replicate, soak in the conidia suspension prepared in advance for 25 seconds, take it out, absorb the water on the body surface with filter paper and put it into a 150mm petri dish , feed fresh mulberry leaves coated with spore suspension on the back of the leaves, place them in a constant temperature incubator at 28°C for moisturizing culture, and treat silkworm and mulberry leaves with sterile water containing 0.02% Tween-80 as a ...

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Abstract

The invention belongs to the field of microorganisms, and in particular relates to a strain of Curvosporium intensis and its application. The Tolypocladium inflatum strain SCKDC10 of the present invention has a deposit number of CCTCC M2018701, and the sequence homology between its rDNA-ITS sequence and Tolypocladium inflatum isolate is as high as 99%. The present invention has the SCKDC10 strain isolated from the larvae of the bat moth, which has good production characteristics and rapid growth, and the diameter of the colony after cultivating for 15 days can reach 10-12 cm; The insect effect is good, when the spore concentration is 2.0 × 10 8 When spores / mL, the cumulative mortality rate of silkworm larvae was 78.33% on the 8th day, and the cumulative mortality rate of Panonychus citrus mite was 59.5%. The insecticidal fungal preparation produced by this strain effectively controlled a variety of pests. total group number.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of C. inflata and its application. Background technique [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in agriculture as an increase in production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (i.e. resistance to pesticides, residence of pesticide residues, No matter how rampant Resurgence is, it has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests. In particular, in the past 10 years, cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites have become more resistant to chemical pesticides such as pyrethroid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N1/14A01N63/30A01P7/02A01P7/04C12R1/645
CPCA01N63/30C07K14/37C12N1/14C12N1/145C12R2001/645
Inventor 秦少容胡军华卿玉玲陈仕江王帆陈若霓
Owner CHONGQING TAIJI MEDICAL RES INST CO LTD
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