Combined plant tissue culture rooting-stage culture medium

A plant tissue culture and combined technology, which is applied in the field of culture medium in the rooting stage of combined plant tissue culture, can solve the problems of single composition of culture medium, influence of plant tissue growth, difficulty in nutritional balance, etc., to achieve beneficial plant growth and effective Good for plant growth and good bactericidal effect

Inactive Publication Date: 2019-01-18
GUANGDONG UNIV OF PETROCHEMICAL TECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to ensure the robust growth of plant tissues, medium is needed. The composition of modern medium is generally relatively simple, and it is difficult to achieve nutritional balance in the process of plant growth. In addition, in order to ensure the growth effect of plants, the medium is generally Sterilization treatment,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment one, a kind of combined type plant tissue culture rooting stage medium, take MS basic medium as basic medium, also comprise following components: banana juice 20g / L, pearl powder 16g / L, agar powder 12g / L, Inositol 0.1g / L, sugar 20g / L, aloe extract 2g / L, bactericidal powder, hormone, alcohol and deionized water;

[0023] The MS basic medium includes macronutrients, micronutrients and organic matter; the macronutrients include the following components and the concentrations of each component are: potassium nitrate 500mg / L, ammonium sulfate 280mg / L, heptahydrate Magnesium sulfate 200mg / L, anhydrous potassium dihydrogen phosphate 20mg / L, disodium edetate 5.5mg / L, ferrous sulfate heptahydrate mg / L; the micronutrients include the following components and each group The concentrations are: calcium chloride 10mg / L, zinc sulfate 8.5mg / L, boric acid 5.2mg / L, sodium molybdate 1.2mg / L, copper sulfate 0.5mg / L, cobalt chloride 0.01mg / L; Each component of said organic matt...

Embodiment 2

[0034] Embodiment two, a kind of combined type plant tissue culture rooting stage medium, with MS basic medium as basic medium, also includes the following components: banana juice 30g / L, pearl powder 18g / L, agar powder 15g / L, Inositol 0.16 / L, sugar 24g / L, aloe extract 2.8g / L, bactericidal powder, hormone, alcohol and deionized water;

[0035] The MS basic medium includes macronutrients, micronutrients and organic matter; the macronutrients include the following components and the concentrations of each component are: potassium nitrate 500mg / L, ammonium sulfate 280mg / L, heptahydrate Magnesium sulfate 200mg / L, anhydrous potassium dihydrogen phosphate 20mg / L, disodium edetate 5.5mg / L, ferrous sulfate heptahydrate mg / L; the micronutrients include the following components and each group The concentrations are: calcium chloride 10mg / L, zinc sulfate 8.5mg / L, boric acid 5.2mg / L, sodium molybdate 1.2mg / L, copper sulfate 0.5mg / L, cobalt chloride 0.01mg / L; Each component of said organi...

Embodiment 3

[0046] Embodiment three, a kind of combined type plant tissue culture rooting stage medium, take MS basic medium as basic medium, also comprise following components: banana juice 35g / L, pearl powder 21g / L, agar powder 19g / L, Inositol 0.24 / L, sugar 29g / L, aloe extract 3.6g / L, bactericidal powder, hormone, alcohol and deionized water;

[0047] The MS basic medium includes macronutrients, micronutrients and organic matter; the macronutrients include the following components and the concentrations of each component are: potassium nitrate 500mg / L, ammonium sulfate 280mg / L, heptahydrate Magnesium sulfate 200mg / L, anhydrous potassium dihydrogen phosphate 20mg / L, disodium edetate 5.5mg / L, ferrous sulfate heptahydrate mg / L; the micronutrients include the following components and each group The concentrations are: calcium chloride 10mg / L, zinc sulfate 8.5mg / L, boric acid 5.2mg / L, sodium molybdate 1.2mg / L, copper sulfate 0.5mg / L, cobalt chloride 0.01mg / L; Each component of said organic ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Finenessaaaaaaaaaa
Finenessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a combined plant tissue culture rooting-stage culture medium. The combined plant tissue culture rooting-stage culture medium adopts an MS basic culture medium as a basic culture medium, and is also prepared from the following components: 20 to 35 g/L of banana juice, 16 to 21 g/L of pearl powder, 12 to 19 g/L of agar powder, 0.1 to 0.24 g/L of inositol, 20 to 39 g/L of white sugar, 2 to 3.6 g/L of an aloe extract, sterilizing powder, hormone, alcohol and deionized water, wherein the alcohol adopts 95 percent alcohol, the MS basic culture medium comprises normal amount nutrient elements, micro nutrient elements and organic maters, and the sterilizing powder is prepared from the following components: 8 to 11 parts of flos lonicerae, 5 to 8 parts of radix stemonae, 8 to 10 parts of dittany bark, 3 to 6 parts of rhizoma atractylodis, 6 to 9 parts of agastache rugosus and 4 to 8 parts of fructus cnidii; and the fineness of the sterilizing powder is 60 to 90 meshes, abalanced nutrition condition can be provided for the growth of plants, the culture medium is ensured to be in a sterile environment for a long term by adding the sterilizing powder, so that the growth of the plants is facilitated; moreover, the sterilizing powder adopts the traditional Chinese medicine ingredients, not only can harm to plant tissues be avoided and the sterilizing effect good, butalso the influence to the environment can be basically avoided, and the preparation method is simple and suitable for the industrialized production.

Description

technical field [0001] The invention relates to a culture medium, in particular to a combined culture medium for rooting stage of plant tissue culture. Background technique [0002] Culture medium refers to a nutrient matrix prepared from a combination of different nutrients for the growth and reproduction of microorganisms, plants or animals. Generally contain several kinds of substances such as carbohydrates, nitrogenous substances, inorganic salts, vitamins and water. The medium is not only the basic material for providing nutrition and promoting cell proliferation, but also the living environment for cell growth and reproduction. There are many types of medium, which can be divided into natural medium, synthetic medium, and semi-synthetic medium according to the source of the prepared raw materials; solid medium, liquid medium, and semi-solid medium according to the physical state; It can be divided into basic medium, selective medium, enriched medium, differential med...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 欧阳乐军李莉梅马铭赛陈凯钊戴发张燕珊胡健文孙同川黄嘉玲布良灏
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap