Combined plant tissue culture rooting-stage culture medium

Abstract

The invention discloses a combined plant tissue culture rooting-stage culture medium. The combined plant tissue culture rooting-stage culture medium adopts an MS basic culture medium as a basic culture medium, and is also prepared from the following components: 20 to 35 g / L of banana juice, 16 to 21 g / L of pearl powder, 12 to 19 g / L of agar powder, 0.1 to 0.24 g / L of inositol, 20 to 39 g / L of white sugar, 2 to 3.6 g / L of an aloe extract, sterilizing powder, hormone, alcohol and deionized water, wherein the alcohol adopts 95 percent alcohol, the MS basic culture medium comprises normal amount nutrient elements, micro nutrient elements and organic maters, and the sterilizing powder is prepared from the following components: 8 to 11 parts of flos lonicerae, 5 to 8 parts of radix stemonae, 8 to 10 parts of dittany bark, 3 to 6 parts of rhizoma atractylodis, 6 to 9 parts of agastache rugosus and 4 to 8 parts of fructus cnidii; and the fineness of the sterilizing powder is 60 to 90 meshes, abalanced nutrition condition can be provided for the growth of plants, the culture medium is ensured to be in a sterile environment for a long term by adding the sterilizing powder, so that the growth of the plants is facilitated; moreover, the sterilizing powder adopts the traditional Chinese medicine ingredients, not only can harm to plant tissues be avoided and the sterilizing effect good, butalso the influence to the environment can be basically avoided, and the preparation method is simple and suitable for the industrialized production.

Description

technical field [0001] The invention relates to a culture medium, in particular to a combined culture medium for rooting stage of plant tissue culture. Background technique [0002] Culture medium refers to a nutrient matrix prepared from a combination of different nutrients for the growth and reproduction of microorganisms, plants or animals. Generally contain several kinds of substances such as carbohydrates, nitrogenous substances, inorganic salts, vitamins and water. The medium is not only the basic material for providing nutrition and promoting cell proliferation, but also the living environment for cell growth and reproduction. There are many types of medium, which can be divided into natural medium, synthetic medium, and semi-synthetic medium according to the source of the prepared raw materials; solid medium, liquid medium, and semi-solid medium according to the physical state; It can be divided into basic medium, selective medium, enriched medium, differential med...

AI Technical Summary

Problems solved by technology

[0003] In order to ensure the robust growth of plant tissues, medium is needed. The composition of modern medium is generally relatively simple, and it is difficult to achieve nutritional balance in the process of plant growth. In addition, in order to ensure the growth effect of plants, the medium is generally Sterilization treatment, in order to ensure that plant tissues are kept in an environment with few bacteria or no bacteria for a long time, sometimes it is necessary to add fungicides. Modern fungicides are generally composed of chemical components, which are not only harmful to the environment, but also harmful to plant tissues. Growth also has some influence