A kind of high-efficiency and rapid propagation method of Coleus coleus

Abstract

The invention belongs to the field of plant tissue culture, and discloses a high-efficiency and rapid propagation method of Coleus coleus, which comprises the following steps: taking the sword-shaped buds newly extracted from the base of a strong mother plant as explants in March to April, and separately Carry out ① the explants are sterilized with 0.1% mercury chloride for 6-8 minutes, ② insert the sterilized explants into the induction and differentiation medium added with 6-BA, CPPU, NAA and cultivate them, ③ put the callus mass or The sword-shaped adventitious bud clusters were inserted into the proliferation medium supplemented with 6-BA, CPPU and NAA, ④The shoot-shaped shoots with a plant height of 30-40 mm and ￠≥5 mm were cut from the proliferating bud clusters or bud clusters, and inoculated in In the rooting medium added with NAA, when the shoot-shaped buds have 2 to 3 leaves, and the mesophyll of some leaves becomes red spots, the seedlings can be hardened and transplanted. The invention provides a high-efficiency and rapid propagation method of S. coleus, which solves the problems of low bud formation rate, low reproduction coefficient, weak or deformed proliferating buds and rooted seedlings, and easy breeding in the rapid propagation process. Bacteria and other bottlenecks restricting the industrialization of the rapid propagation of S. coleus can obtain a large number of high-quality S. The cost of industrialized seedling cultivation of rib grass realizes the industrialization of high-efficiency and rapid propagation of rib grass.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a high-efficiency and rapid propagation method of Coleus coleus. Background technique [0002] Aglanonema spp is a perennial plant of the genus Aglanonema in the family Araceae, and is a horticultural cultivar of the genus Aglanonema. [0003] At present, the Coleus coleus that has been recorded is basically cross-breeding by Thai breeders, such as Aglaonema 'Favonian'., Aglaonema 'super red', and Lady's Aglaonema 'Lady Valentine', Aglaonema 'super pink', Aglaonema 'red zircon' and Aglaonema 'saim daimond', Aglaonema 'mangmee', Aglaonema' chaang dang', etc., because the leaves of this type of Coleus scutellaria are widely distributed with yellow spots, pink spots, red spots, or basically all red or all yellow, the overall appearance is beautiful and full of joy, and it is the main flower that is widely popular in the market . [0004] The propagation methods of Coleus coleus ...

AI Technical Summary

Problems solved by technology

For example, in the reference literature Aglaonema spp tissue culture and rapid propagation, the terminal buds and stem segments of the robust Aglaonema spp mother plant were used as explants, MS was used as the basic medium, and 2-ip was used as the cell division hormone , the induction rate of adventitious buds in stem segments is up to 95.1%, the terminal buds are 93.5%, and the proliferation rate is 2 to 4. However, it can be seen from the attached drawings that the explants are aging, the callus formed by dedifferentiation is slow and aging, and the adventitious buds on the bud mass Many deformities, basically all bud clusters can only take out one dominant bud, the budding rate is less than 5%, the budding rate is weak, the seedlings are deformed, the budding rate is too low, the multiplication cycle is too long, and the cost of rapid propagation is high, which is not conducive to the industry change

In patent CN103975853B, the aboveground part of the strong and colorful Sclerosia auspicious plant is used as explant, the improved MS is used as the basic medium, 6-BA, 2-ip, KT are used as cell division hormones, and triiodobenzoic acid is used as the top growth inhibitor , combined with the puncture cutting method of increasing the contact surface of the growth point, the proliferation rate reached 4.0, and the rooting rate reached more than 95%. Many buds are deformed, most of the bud clusters can only pick up one dominant bud, the budding rate is less than 5%, the budding rate is too small, the seedlings are deformed, the budding rate is too low, the multiplication cycle is too long, and the cost of rapid propagation is high, which is still not conducive to the industry change

Although the young stems were used as explants, due to the effect of apical dominance, the cryptobuds on the young stems were still in a semi-dormant state, and increasing the concentration of the cell division hormone 6-BA could accelerate the division of cryptobud cells to a certain extent, but The buds are still slow, and the buds are weak, the multiplication speed is slow, and the cost of rapid propagation is high, which is still not conducive to industrialization

[0006] The above-mentioned method of tissue culture and rapid propagation of Sclerostifolia or colored Scleracea, references and patent CN103975853B are all methods of rapid propagation of adventitious buds induced by callus, but due to the aging of explants, the formation of callus and adventitious buds is slow, The adventitious buds are deformed, the budding rate is low, the buds are weak or deformed, the seedling rate is low, the multiplication rate is not high, the multiplication cycle is long, the cost of rapid propagation is high, and it is not conducive to industrial multiplication; moreover, aging explants are easy to parasitize eubacteria , leading to high pollution in the later stage of multiplication and large waste of materials

Both patent CN107711513A and patent CN101536674B are budding and rapid propagation methods, but because the cryptobuds on the explants are in a semi-dormant state, although the treatment of breaking dormancy and apex dominance is performed, it takes a long time for the cryptobuds to start up, and the buds are weak. The multiplication speed is slow and the cost of rapid multiplication is high, which is still not conducive to industrial multiplication