The invention belongs to the field of
plant tissue culture, and discloses a high-efficiency and rapid propagation method of
Coleus coleus, which comprises the following steps: taking the sword-shaped buds newly extracted from the base of a strong mother
plant as explants in March to April, and separately Carry out ① the explants are sterilized with 0.1% mercury
chloride for 6-8 minutes, ② insert the sterilized explants into the induction and differentiation medium added with 6-BA, CPPU, NAA and cultivate them, ③ put the
callus mass or The sword-shaped adventitious
bud clusters were inserted into the proliferation medium supplemented with 6-BA, CPPU and NAA, ④The
shoot-shaped shoots with a
plant height of 30-40 mm and ¢≥5 mm were
cut from the proliferating
bud clusters or
bud clusters, and inoculated in In the rooting medium added with NAA, when the
shoot-shaped buds have 2 to 3 leaves, and the mesophyll of some leaves becomes red spots, the seedlings can be hardened and transplanted. The invention provides a high-efficiency and rapid propagation method of S.
coleus, which solves the problems of low bud
formation rate, low
reproduction coefficient, weak or deformed proliferating buds and rooted seedlings, and easy breeding in the rapid propagation process.
Bacteria and other bottlenecks restricting the industrialization of the rapid propagation of S.
coleus can obtain a large number of high-quality S. The cost of industrialized
seedling cultivation of rib grass realizes the industrialization of high-efficiency and rapid propagation of rib grass.