Preparation method of dried sweet potatoes rich in probiotics
A probiotic-rich, dried sweet potato technology, which is applied in the field of preparation of probiotic-rich dried sweet potatoes, can solve the problems of weak impregnation of probiotic strains, utilization of probiotics and sweet potatoes, and few active strains, so as to achieve rich taste and nutrition The effect of good retention of ingredients and firm impregnation
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Embodiment 1
[0030] A method for preparing dried sweet potatoes rich in probiotics, using dried sweet potatoes as a carrier for enriching probiotics, and adopting a dipping method for bacteria enrichment treatment, comprising the following steps:
[0031] (1) Preparation of probiotic dipping solution: transfer Lactobacillus acidophilus and Bifidobacterium longum preserved at low temperature into MRS medium, and pick pure colonies after identification to make a concentrated bacterial suspension for preparing glycerol cryovials At the same time, pick the pure colony and connect the slant culture medium in the storage tube, which is the third generation of probiotics, and then transfer it to a 200ml Erlenmeyer flask containing MRS liquid medium at a ratio of 1:1 with a 2% inoculum. Expand the culture at ℃ for 12 hours, pour the bacterial solution into a centrifuge tube, then centrifuge at a speed of 4000r / min for 15 minutes, then dilute the precipitated bacteria with 10% sucrose solution to ma...
Embodiment 2
[0039] A method for preparing dried sweet potatoes rich in probiotics, using dried sweet potatoes as a carrier for enriching probiotics, and adopting a dipping method for bacteria enrichment treatment, comprising the following steps:
[0040] (1) Preparation of probiotic dipping solution: transfer Lactobacillus johnsonii, aerobic bifidobacteria, Lactobacillus fermentum, Lactobacillus reuteri, and Streptococcus thermophilus preserved at low temperature into MRS medium, and identify Then pick pure colonies to make a concentrated bacterial suspension for the preparation of glycerol cryovials, pick pure colonies and connect the slant culture medium at the same time as the third-generation probiotics, and then use 2% inoculum at a ratio of 1:2 Transfer to a 250ml Erlenmeyer flask containing MRS liquid medium, expand culture at 37°C for 18 hours, pour the bacterial solution into a centrifuge cup, then centrifuge at a speed of 4500r / min for 13min, then dilute the precipitated bacteria...
Embodiment 3
[0048] A method for preparing dried sweet potatoes rich in probiotics, using dried sweet potatoes as a carrier for enriching probiotics, and adopting a dipping method for bacteria enrichment treatment, comprising the following steps:
[0049] (1) Preparation of probiotic dipping solution: Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus johnsonii, aerobic bifidobacterium, Lactobacillus fermentum, Lactobacillus reuteri, Streptococcus thermophilus preserved at low temperature The cocci were transferred into the MRS medium, and after identification, the pure colonies were picked to make a concentrated bacterial suspension for the preparation of glycerol cryovials, and the pure colonies were picked and connected to the slant medium in the storage tubes, which were the third generation probiotics, and then press 1 The ratio of : 2 was transferred to a 500ml Erlenmeyer flask containing MRS liquid medium with a 2% inoculation amount, expanded at 37°C for 24 hours, pour...
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