Bacillus subtilis used for antagonizing strawberry gray mold, and preparation and application of antibiological inoculant thereof
A technology of Bacillus subtilis and strawberry gray mold, applied in the field of microbiology, can solve problems such as endangering the safety and health of humans and animals, limited fungicides, and reduced control effects, and achieves good development and application prospects, easy industrial production, and simple preparation methods Effect
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Embodiment 1
[0029] Embodiment 1: a strain is used for antagonizing Bacillus subtilis of strawberry gray mold ( Bacillus subtilis ) new strain WZ, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, the preservation number is CGMCC No. 16418, and the preservation date is for September 4, 2018.
[0030] Collection, Isolation and Identification of Bacillus subtilis WZ Strain
[0031] (1) Bacillus subtilis WZ was isolated from the strawberry gray mold fruit collected in Jurong Baitu Town, Zhenjiang. First, the unaffected part of the strawberry fruit suffering from gray mold was cut, and the surface was disinfected with 70% alcohol. Grinding was then carried out under sterile conditions; screening was carried out in LB medium by serial dilution;
[0032] (2) After a single colony appears on ...
Embodiment 2
[0040] Embodiment 2 Preparation of biocontrol agent
[0041] (1) Inoculate the biocontrol strain WZ into LB culture medium, culture at 37°C, 220 r / min shaking, after 24-36 hours, take samples in the ultra-clean workbench and measure the OD value at 600 nm;
[0042](2) When the OD value of the bacterial liquid is between 0.6 and 0.8 after shaking culture, it is the seed bacterial liquid, and inoculated into the LB culture liquid with an inoculum of 1% (the composition of the culture liquid is: beef extract 5 g / L, peptone 10 g / L, sodium chloride 5 g / L, pH 7.2-7.4), the temperature of the culture solution is 37°C, the number of days is 2 days, the rotation speed is 220 rpm, and the pH value is 7.2-7.4. After the fermentation culture, add 5% sucrose, then filter and adjust the cell number of the bacterial solution to 10 9 Individual / mL, promptly make the biocontrol agent of Bacillus subtilis WZ of the present invention.
Embodiment 3
[0043] Example 3 Effect of biocontrol fungicide WZ on postharvest natural rot rate of strawberry
[0044] Prepare 1×10 separately 7 , 1×10 8 , 1×10 9 CFU / mL of Bacillus subtilis suspension, with sterile water as the control. Strawberry fruits were directly soaked in the above three different concentrations of antagonistic bacteria suspensions or sterile water for 1 min without disinfection and washing, dried and stored at 20°C. After 4 days, the fruit rot rate was measured. Each group treated 30 fruits and repeated 3 times.
[0045] The result is as Figure 4 As shown, it can be seen from the figure that different concentrations of biocontrol bacteria have a certain inhibitory effect on the rot of strawberry fruit, and with the increase of the concentration of the bacterial suspension, the inhibitory effect on the rot of strawberry fruit is more obvious, indicating that The biocontrol fungus can be used in post-harvest preservation of strawberries.
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