RNA extraction solution, method for preparing RNA extraction solution, and RNA reagent extraction method

Abstract

The invention discloses an RNA extraction solution, a method for preparing the RNA extraction solution, and an RNA reagent extraction method. The RNA extraction solution comprises the mass fraction orthe molar concentration of the following components: 30-50% by volume relative to the total amount of the solution; The total amount of the solution is 3 to 10% by volume of the polyol; the cerium salt is 0.5 to 2 M in concentration relative to the total amount of the solution; 0.1 to 1% by volume of the sodium lauryl sarcosinate relative to the total amount of the solution With respect to the total amount of the solution of 0.05-0.2M concentration of acetic acid; relative to the total amount of the solution 0.05-0.2M of sodium acetate, the remaining component is ultrapure water, in the present invention through acetic acid The ratio of sodium acetate to the pH of the control buffer can completely separate DNA and RNA, and avoid the disintegration of RNA under acidic conditions. It also combines the convenience of silica gel membrane adsorption column and simplifies. The subsequent steps of RNA extraction can be completed by shortening to about 10 minutes compared to the previous onehour or more.

Description

technical field [0001] The invention relates to the biomedicine industry, in particular to an RNA extraction solution, a method for preparing the RNA extraction solution, and an RNA reagent extraction method. Background technique [0002] Ribonucleic acid (abbreviated as RNA, Ribonucleic Acid) is a genetic information carrier that exists in biological cells and some viruses and viroids. RNA is a long chain molecule formed by condensation of ribonucleotides through phosphodiester bonds. RNA is a single strand formed by transcription based on a strand of DNA as a template and based on the principle of complementary base pairing. RNA can regulate the phenotype of organisms through properties such as type and expression level. In order to conduct gene expression analysis and research, it is very important to extract high-purity RNA from various biological samples. Many methods have been developed to obtain RNA from biological materials, such as phenol extraction, precipitation...

AI Technical Summary

Problems solved by technology

However, in the case of liquid treatment with DNase, in order to remove DNase after treatment, it is necessary to perform phenol / chloroform extraction or protein denaturation experiment again

In addition, it can be processed in combination with a silica membrane adsorption column, but the steps of washing the adsorption column must be repeated

These methods not only increase the amount of labor when removing impurity DNA, but also cause the loss of RNA, thereby reducing the amount of RNA extracted.