Method for creating antiretroviral gene alfalfa

Abstract

The invention discloses a method for creating an antireversal gene alfalfa. The method comprises: based on a plant expression vector P3300, and comprises the steps of constructing a plant expression vector P3300; NHX-CAX plant expression vector and corresponding Agrobacterium tumefaciens transformants were obtained. The CAX gene and NHX gene were simultaneously expressed in transgenic alfalfa plants by double gene / single plasmid / Agrobacterium tumefaciens co-transformation, and the transformation efficiency was 15%. Finally, 318 transgenic alfalfa plants containing both CAX gene and NHX gene were obtained, and the target gene could be expressed at RNA level, which resulted in the creation of new transgenic alfalfa germplasm with significantly enhanced drought resistance and salt tolerance.It provides a powerful theoretical basis for the application of polygene polymerization to improve the stress resistance of crops and forages, It is of great practical significance to increase the yield of crops and herbage, increase the income of farmers and herdsmen, alleviate the crisis of water resources, improve and utilize large areas of saline-alkali wasteland, promote the production of agriculture and animal husbandry, and ensure the high yield of grain.

Description

technical field [0001] The invention belongs to the technical field of transgenic research on alfalfa, and in particular relates to a stress-resistant multi-gene polymerization transformation method of alfalfa. Background technique [0002] The Songnen Plain is one of the three largest soda saline-alkali land distribution areas in the world, covering an area of ​​2.39 million hectares, of which 667,000 hectares of saline-alkali land in Jilin Province urgently need to be treated. Drought and salinization have become the main environmental factors restricting crop yield. Especially in the western part of Jilin Province, due to the impact of global climate change and the interference of human production activities, soil drought and salinization have posed a real threat to the growth of most crops and pastures, seriously affecting local sustainable development. Therefore, Breeding excellent plant varieties that are both salt-alkali tolerant and drought-resistant is an important...

AI Technical Summary

Problems solved by technology

With the in-depth study of plant stress-resistant genetic engineering, more and more functional genes have been introduced into alfalfa to obtain high-stress-resistant alfalfa materials. The traits are controlled by multiple genes, and its molecular mechanism is somewhat complicated. At present, most of the anti-reversal gene research in alfalfa is the transfer of a single gene that controls a single trait.

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