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Electrochemical chiral recognition detection method for lorazepam

A detection method and chirality technology, applied in the field of electrochemical detection, can solve the problems of complicated preparation of chiral columns, expensive instruments, and time-consuming analysis, and achieve enhanced stability and conductivity, rapid identification process, and improved sensitivity. Effect

Active Publication Date: 2019-01-18
山东信开源科技创新发展有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the chromatographic method for identifying lorazepam also has many shortcomings, for example, the preparation of the chiral column is complicated, the price is relatively expensive, the instrument is expensive, and the analysis takes a long time, etc.

Method used

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  • Electrochemical chiral recognition detection method for lorazepam

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of graphene: graphene oxide is prepared by Hummers method, take 1g graphene oxide, then add 3gNaBH 4 , with Na 2 CO 3 Adjust the pH to 8, heat in a water bath at 90°C for 1 hour, filter, wash repeatedly with ethanol and distilled water, dry in vacuum at 50°C for 12 hours, and set aside;

[0029] (2) Carboxylation modification of graphene: 2 g of the prepared graphene material was added to 20 mL of concentrated nitric acid, ultrasonically reacted for 10 min, filtered, washed several times with ethanol and distilled water, and dried to obtain carboxylated graphene.

Embodiment 2

[0031] Preparation of graphene: graphene oxide is prepared by Hummers method, take 2g graphene oxide, then add 6gNaBH 4 , with Na 2 CO 3 Adjust the pH to 8, heat in a water bath at 90°C for 3 hours, filter, wash repeatedly with ethanol and distilled water, dry in vacuum at 50°C for 24 hours, and set aside;

[0032] Carboxylation modification of graphene: Take 4 g of the prepared graphene material and add it to 20 mL of concentrated nitric acid, ultrasonically react for 15 min, filter, wash with ethanol and distilled water several times, and dry to obtain carboxylated graphene.

Embodiment 3

[0034] Preparation of carboxylated graphene electrode: ultrasonically disperse carboxylated graphene into an aqueous solution containing 0.5% Nafion, apply an appropriate amount drop-coated on the surface of an activated glassy carbon electrode, and dry under infrared light to obtain carboxylated graphene-modified glass carbon electrode;

[0035] Electrochemical modification of poly-L-leucine: a three-electrode system in which a carboxylated graphene-modified glassy carbon electrode is used as a working electrode, silver / silver chloride is used as a reference electrode, and a platinum electrode is used as a counter electrode is immersed in 0.1mol / L In an aqueous solution of L-leucine, L-leucine is electrochemically polymerized and modified on the electrode surface by cyclic voltammetry scanning to obtain a poly-L-leucine / carboxylated graphene / glassy carbon electrode, and the scanning voltage is - 0.2-1.5V, the scan rate is 100mV / s.

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Abstract

The invention discloses a chiral detection method for lorazepam, realized by adopting the following steps: preparing graphene, performing carboxylation on the graphene and modifying on a glassy carbonelectrode, then electrochemically modifying poly-L-leucine, detecting a lorazepam isomer by adopting a differential pulse voltammetry procedure, and scanning a potential from -0.3V to 0.6V, wherein results show that R-lorazepam has a relatively high peak current and S-lorazepam has a relatively low peak current. The detection method disclosed by the invention can realize fast recognition of R-lorazepam and S-lorazepam, and the detection method is simple and high in detection efficiency.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, in particular to an electrochemical chiral recognition detection method for lorazepam. Background technique [0002] Lorazepam, also known as lorazepam and lorazepam, is a benzodiazepine drug commonly used clinically for anxiolytic, anticonvulsant, sedative, hypnotic, and relief of alcohol withdrawal symptoms, etc. Its effect is similar to that of diazepam but stronger than that of diazepam. It possesses a pair of enantiomers. According to the relevant laws and regulations of the country, chiral drugs need to investigate the efficacy and toxicology of their monomers, so chiral recognition of lorazepam is required. [0003] At present, there are many technologies and methods for chiral recognition, and chromatographic analysis is a large and most important class of chiral recognition methods, which has become the main means of modern chiral resolution and recognition, including gas chrom...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30C01B32/194C01B32/184
CPCC01B32/184C01B32/194G01N27/308G01N27/48
Inventor 孟青
Owner 山东信开源科技创新发展有限责任公司
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