Core-shell coaxial nanofibers for repairing periodontitis bone defects, preparation method thereof and use thereof

A technology of nanofibers and nanofiber membranes, applied in the direction of non-active ingredient medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve good anti-inflammatory effects

Inactive Publication Date: 2019-01-25
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current clinical treatment methods are d

Method used

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  • Core-shell coaxial nanofibers for repairing periodontitis bone defects, preparation method thereof and use thereof
  • Core-shell coaxial nanofibers for repairing periodontitis bone defects, preparation method thereof and use thereof
  • Core-shell coaxial nanofibers for repairing periodontitis bone defects, preparation method thereof and use thereof

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Experimental program
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Embodiment

[0046] The specific process of preparing core-shell coaxial nanofibers for repairing periodontitis bone defects is as follows:

[0047] Weigh 5mg of resveratrol and 50mg of Pluronic F127 and fully dissolve in 10ml of absolute ethanol; remove the organic solvent by rotary evaporation to form a thin film, remove the residual organic solvent by vacuum suction overnight, and hydrate with 30ml of deionized water , magnetically stirred at room temperature for 4 hours, filtered through a 0.22 μm filter membrane, and freeze-dried to prepare resveratrol-loaded micelles (RES-PMs). figure 1 and 2 shown.

[0048] See attached image 3 Core-shell coaxial nanofiber membranes loaded with resveratrol and BMP-2 were prepared by coaxial electrospinning, and the 5mL syringes filled with resveratrol micellar solution and BMP-2 solution were respectively fixed on the syringe pump. On the side and the top, connect the nozzle, adjust the distance between the nozzle and the receiving plate, start ...

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Abstract

The invention discloses a core-shell coaxial nanofiber for repairing a periodontitis bone defect, a preparation method thereof and a use thereof. Pluronic F127 is taken as a raw material, resveratrolis taken as a model drug, resveratrol polymer micelles is prepared by a film hydration process, and core-shell coaxial nanofibers loaded with resveratrol and bone morphogenetic protein-2 (BMP-2) is prepared by coaxial electrospinning. Because the core and shell layers of the fiber were loaded with drugs with different biological activities and functions at the same time, the drug release order andconcentration in the core and shell layers are adjusted by regulating the structure of nanofibers, the combined use of drugs is achieved, the inhibition of lipopolysaccharide (LPS)-induced inflammatory cytokines expression in periodontal ligament cells and the inhibition of osteoclast differentiation in RAW264.7 cells provide a new approach for the treatment of periodontal disease.

Description

technical field [0001] The invention belongs to the field of slow-release biomaterials, and in particular relates to core-shell coaxial nanofibers for repairing periodontitis bone defects, a preparation method and application thereof. Background technique [0002] Periodontitis is a chronic inflammatory disease caused by local factors. The infiltration and development of inflammation eventually lead to alveolar bone resorption, tooth loosening and loss. , LPS) is an important pathogenic factor in the progression of periodontitis. Immunological studies have found that LPS can increase the expression of pro-inflammatory factors such as interleukin-1β (IL-1β) and interleukin-8 (IL-8) through the body's innate immune response, thereby promoting osteoclast differentiation and maturation, and ultimately Cause alveolar bone resorption. Therefore, inhibiting the expression of inflammation-related factors is a prerequisite for preventing osteoclastogenesis. [0003] Osteoclasts ar...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K9/70A61K47/10A61K38/18A61P1/02A61K31/05
CPCA61K9/0092A61K9/1075A61K9/7007A61K31/05A61K38/1875A61K47/10A61P1/02A61K2300/00
Inventor 解健苏俭生
Owner TONGJI UNIV
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