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Net house cultivation method for basic seedlings of virus-free strawberry shoot-tips

A technology for detoxification of shoot tips and strawberry stolons, applied in fertilization methods, horticultural methods, botanical equipment and methods, etc., can solve problems such as offspring variation, accumulation of plant hormones, and difficulty in prevention and control, so as to reduce production risks and improve economic benefits. , to avoid the effect of offspring mutation

Inactive Publication Date: 2019-01-29
淮南市润吉生态农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once infected with the virus, it is difficult to prevent and treat it with conventional chemicals, which greatly restricts the development of the strawberry industry
Usually, virus-free strawberry seedlings proliferate in culture bottles, which can easily lead to the accumulation of plant hormones and cause variation in offspring, such as difficulty in flowering, low yield, and many deformed fruits, which bring great risks to strawberry production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] A method for cultivating the original seedlings of strawberry stem tip detoxified in a net room, comprising the following specific steps:

[0018] (1) Select strawberry stolons that germinate in June and grow fully, and the leaflets have not yet unfolded. The top of the 4 cm long stolon is disinfected, and the sterilized strawberry stolon is placed under a dissecting microscope, and the tip of the strawberry is stripped. Immediately place the growing point on the starting medium, and after 15 minutes of pressure sterilization at 121°C, place the culture at a temperature of 25°C, light of 1500Lx, and sunshine hours of 15h to start the culture;

[0019] (2) Put the test-tube seedlings into the greenhouse before transplanting, harden the seedlings under natural light for 3 days, and then unscrew the bottle for 2 days. When the tissue-cultured seedlings have no abnormal reaction, transplant them to In the seedling plug tray, irrigate with water after transplanting, and put ...

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Abstract

The present invention discloses a net house cultivation method for basic seedlings of virus-free strawberry shoot-tips. According to the present invention, strawberry test-tube plantlets are grown through heat treatment combined with shoot-tip tissue culture, and then directly acclimatized and transplanted without being propagated in test tubes. Virus detection is carried out on the test-tube plantlets survived after the transplanting by means of an indicative plant method. The obtained virus-free basic seedlings are bred in a net house. The strawberry seedlings which are previously subjectedto detoxification detection are propagated in a stolon mode through the net house, thus offspring variation caused by propagation in an original test tube is avoided and the breeding of strawberry seedlings is facilitated. The cultivation method for strawberry in the present invention has the advantages of low variation, reduced production risk and increased economic benefit as compared with multi-generation propagation of test-tube plantlets alone.

Description

technical field [0001] The invention relates to the technical field of vegetable and fruit planting, in particular to a method for cultivating strawberry stem tips in net chambers for original seedlings detoxified. Background technique [0002] Strawberry is a perennial herb of the Rosaceae Strawberry genus. The fruit is sweet and sour, and is known as the "Queen of Fruits". The fruit matures early, and greenhouse cultivation can supply the whole winter market, filling the off-season fruit. At the same time, strawberry is also an important food processing raw material. Strawberry is a crop that reproduces asexually by stolons. After several generations, it is susceptible to various viruses. Strawberries infected with viral diseases grow slowly, with shrunken leaves, small and deformed fruits, and poor quality. There are mainly four kinds of strawberry virus diseases that have been identified in my country, namely strawberry mottle virus, strawberry light yellow edge virus,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/05A01G2/30A01G7/06A01G13/00A01G31/00A01G24/28A01G24/15A01H4/00A01C21/00
CPCA01C21/005A01G2/30A01G7/06A01G13/00A01G22/05A01G24/15A01G24/28A01G31/00A01H4/001A01H4/008
Inventor 张士军孙伟
Owner 淮南市润吉生态农业有限公司