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Frog-derived wound repair peptide and synthesis method thereof

A wound repair and synthesis method technology, applied in the field of biomedicine, can solve the problems of reduced yield of target polypeptide, low renaturation efficiency, hindered healing, etc., and achieves the effect of simple operation and high yield

Pending Publication Date: 2019-01-29
武汉帕肽生物医药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(5) Blood supply: After tissue damage, local capillaries are also damaged, or other factors cause vascular sclerosis, etc., which will lead to insufficient blood supply and tissue malnutrition, hindering healing
Due to the addition of disulfide bond remodeling and HPLC purification steps, it often leads to lower renaturation efficiency and lower yield of target peptides

Method used

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  • Frog-derived wound repair peptide and synthesis method thereof
  • Frog-derived wound repair peptide and synthesis method thereof
  • Frog-derived wound repair peptide and synthesis method thereof

Examples

Experimental program
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preparation example Construction

[0061] The present invention also provides a method for synthesizing the frog wound repair peptide shown in SEQ ID No.1, characterized in that the method mainly includes the following steps:

[0062] The Fmoc solid-phase synthesis method is adopted, and the resin is used as a solid-phase carrier, which is activated by a condensing agent. The first amino acid (position 1, counted from the C-terminal to the N-terminal) connected to the solid-phase resin is a diaminodiacid, and the amino acids are coupled one by one from the C-terminal to the N-terminal according to the sequence shown in SEQ ID No.1. on the resin. After the amino acid in the sequence shown in SEQ ID No.1 is condensed from the C-terminal to the 6th glycine at the N-terminal, the protecting group of the diaminodiacid is removed using a removing reagent. Afterwards, the diaminodiacid at the first position from the C-terminal to the N-terminal is cyclized and condensed with the amino acid at the sixth position. Nex...

Embodiment 1

[0084] Example 1: Conventional preparation method of frog wound repair peptide as shown in SEQ ID No.1

[0085] The amino acid sequence of one of the frog wound repair peptides provided in this application is shown in SEQ ID No. 1, which is LVGKL LKGAV GDVCG LLPIC. In the routine synthesis of frog wound repair peptides, linear peptides need to be synthesized first, and a denaturation process is required after cleavage.

[0086] The flowchart of the traditional method for synthesizing frog wound repair peptide as shown in SEQ ID No.1 is as follows figure 1 shown in .

[0087] The specific steps of synthesis are as follows:

[0088] Weigh 2-Cl-Trt resin or wang resin (0.125mmol) into a solid-phase synthesis tube, and use DCM / DMF to swell the resin for 15-30min. Drain the solvent, wash with DCM, and carry out condensation reactions in sequence according to the polypeptide sequence. Polypeptide chain synthesis (Fmoc-amino acid 0.4mmol, HCTU 0.38mmol, DIEA 8mmol) was carried ou...

Embodiment 2

[0093] Example 2: The frog wound repair peptide shown in SEQ ID No.1 was synthesized by the method of the present application

[0094] The amino acid sequence of the frog wound repair peptide provided by this application is shown in SEQ ID No.1, which is LVGKL LKGAV GDVCG LLPIC, and wherein the S atom on the first Cys from the C-terminal to the N-terminal is replaced by a C atom Replaced, thus causing the S-S bond between the 1st and 7th positions from the C-terminal to the N-terminal to be replaced with a C-S bond.

[0095] The flow chart of the synthesis of frog wound repair peptide shown in SEQ ID No.1 is as follows Figure 4 shown in .

[0096] The specific steps of synthesis are as follows:

[0097] Weigh 2-Cl-Trt resin (0.125 mmol) into a solid-phase synthesis tube, and use DCM / DMF to swell the resin for 15-30 min. Drain the solvent, wash with DCM, and carry out condensation reactions in sequence according to the polypeptide sequence.

[0098] The first amino acid (p...

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Abstract

The invention provides a new frog-derived wound repair peptide and a synthesis method thereof. The synthesis method is simple and convenient to operate, the obtained frog-derived wound repair peptidedirectly is a cyclic peptide after synthesis, and does not need to undergo a denaturation and renaturation process. At the same time, the target polypeptide can be obtained in a high yield through thesynthesis method. Furthermore, C-S bonds are introduced into the frog-derived wound repair peptide prepared by the method to replace S-S bonds which are easily broken, so that the spatial conformation of the protein is stabilized, and the good stability of the frog-derived wound repair peptide is obtained.

Description

technical field [0001] The application belongs to the field of biomedicine and relates to a new polypeptide and its synthesis method. More specifically, it relates to a new frog wound repair peptide and its synthesis method. Background technique [0002] Cell and tissue damage can be caused by many factors. Surviving healthy cells in and around tissue sites after injury continue to divide and proliferate to replace dead cells and repair damaged tissue. This physiological function of organisms is called regeneration and repair. [0003] Normally, different organs in the body have different abilities to repair and regenerate. Epidermal cells (such as the mucosal epithelium of the respiratory tract, digestive tract, and genitourinary organs), lymphocytes, and hematopoietic cells are cells with strong regenerative capabilities. Regenerative repair ability, so it belongs to cells with strong regenerative ability. Others such as vascular endothelial cells, periosteum cells, ce...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/16C07K1/06C07K1/04A61K8/64A61K38/10A61Q19/00A61P17/02A61P17/00
CPCA61K8/64A61K38/00A61Q19/00A61P17/00A61P17/02C07K7/08Y02P20/55
Inventor 郭晓奇肖亮李冬
Owner 武汉帕肽生物医药有限责任公司
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