Chitosan microsphere composition loaded with stem cell active factors and preparation method and application thereof

A technology of chitosan microspheres and active factors, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of low transdermal absorption and delivery efficiency of stem cell active factor skin care products, and achieve continuous protection effect , Enhanced utilization, skin roughness improvement effect

Pending Publication Date: 2019-02-05
首玺(广州)医疗科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a chitosan microsphere composition loaded with stem cell active factors and its preparation method and application. The composition can be added to various cosmetics

Method used

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  • Chitosan microsphere composition loaded with stem cell active factors and preparation method and application thereof
  • Chitosan microsphere composition loaded with stem cell active factors and preparation method and application thereof
  • Chitosan microsphere composition loaded with stem cell active factors and preparation method and application thereof

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preparation example Construction

[0032] First aspect, a kind of preparation method of the chitosan microsphere composition that is loaded with stem cell activity factor provided by the present invention, comprises the steps:

[0033] (1) Stem cells are cultured and amplified with clinical-grade serum-free medium, and the culture supernatant is collected after regular medium change and passage, and the stem cell active factor solution is obtained after removing cells and cell debris;

[0034](2) Add lactic acid to the stem cell activating factor solution obtained in step (1), then add chitosan oligosaccharides, and form a polycation mixed solution after stirring, wherein the volume ratio of the lactic acid to the stem cell activating factor solution is 0.5 ~5:100, the ratio of the mass of chitosan to the volume of the stem cell activating factor solution is 0.5~1:100 (g / mL);

[0035] (3) Add the polyanion solution into the polycation mixed solution obtained in step (2) under stirring, and react to obtain the c...

Embodiment 1

[0057] Example 1 Preparation of Umbilical Cord Mesenchymal Stem Cell Active Factor Solution

[0058] (1) Isolation of umbilical cord mesenchymal stem cells: place the umbilical cord that has passed the quality inspection (sterile, negative for infectious disease, and relatively hypertrophic in appearance) in a petri dish, and peel off the outer skin and blood vessels under aseptic conditions; Wash off the residual blood with the gel-like substance, and cut the tissue into a clean culture dish; wash with DMEM medium, add mesenchymal stem cell serum-free medium to resuspend, add it to a T75 culture bottle and culture it in a cell culture incubator ;

[0059] (2) Expansion of mesenchymal stem cells: the primary cells are subcultured after they grow to 50-80% confluent, and then subcultured every 2-3 days until P5;

[0060] (3) Collect the supernatant of stem cell activity factor: P1-P5 generation cells collect the cell culture supernatant before each passage, mix and filter (0.4...

Embodiment 2

[0072] The present embodiment provides a kind of preparation method of the chitosan microsphere suspension that is loaded with stem cell active factor, comprises the steps:

[0073] According to the method of Example 1, the stem cell activating factor solution was obtained, and 1 L of the resulting stem cell activating factor solution was measured. Under aseptic conditions, 10 ml of lactic acid was added to the stem cell activating factor solution and magnetically stirred at a speed of 100 rpm for 2 minutes, followed by adding 5g of chitosan oligosaccharides was stirred magnetically at a speed of 100rpm for 10min to dissolve chitosan oligosaccharides to obtain a mixed solution; under magnetic stirring conditions of a speed of 100rpm, 100ml of sodium tripolyphosphate Solution (mass concentration is 1wt%) is uniformly sprayed in the mixed solution of gained, after preparation, continue to stir 30min and make microsphere harden, the solution that prepares is the chitosan microsphe...

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Abstract

The invention discloses a chitosan microsphere composition loaded with stem cell active factors and a preparation method and application thereof. The chitosan microsphere composition loaded with the stem cell active factors is characterized in that chitosan is added to and dissolved in a stem cell active factor solution and prepared into active factor-embedded chitosan microspheres, which are capable of effectively reducing the compaction of a horny layer structure, and affecting a microstructure of keratin to be loose and generate microchannels, thereby dredging channels for percutaneous absorption and transmission of the stem cell active factors, enhancing the utilization ratio of the stem cell active factors, enabling the active factors to enter skin corium more easily, and exerting a protective effect on skin. The chitosan microsphere composition loaded with the stem cell active factors can be extensively applied to cosmetics, and is helpful to percutaneous absorption and transmission of the stem cell active factors, and the cell factors can continuously release in a body to maintain a lasting skin protecting effect owing to a slow-release effect of the microspheres.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a composition of chitosan microspheres loaded with stem cell active factors, a preparation method and application thereof. Background technique [0002] Stem cells are a type of cells capable of self-replication and multi-directional differentiation potential, and have been widely used clinically in recent years. At present, more than a thousand neural stem cell therapy projects have been registered on the international clinical trial registration website, mainly for cerebral palsy, cerebral apoplexy (such as cerebral infarction, cerebral hemorrhage) sequelae, spinal cord injury, motor neuron disease, cerebellar atrophy, mutual aid Deliberation, Parkinson's disease, optic atrophy, Alzheimer's and other diseases. And more than ten stem cell products have been approved for marketing worldwide, distributed in South Korea, the United States, Canada, Europe, Australia and other cou...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/73A61K8/64A61K8/11A61Q19/00
CPCA61K8/99A61K8/11A61K8/64A61K8/736A61Q19/00
Inventor 钟慧霖刘志成丛宁李静仪陈德
Owner 首玺(广州)医疗科技有限责任公司
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