Lactobacillus fermentum cd110 and its application in the preparation of fermented sausage

A technology of fermenting Lactobacillus and starter, applied in the field of microorganisms, can solve the problems of losing the unique flavor of fermented products, difficulty in ensuring product quality, and complicated sources of microorganisms, so as to reduce the risk of spoilage and mildew, shorten the fermentation cycle, improve Effects on Flavor and Quality

Active Publication Date: 2021-06-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, there are some problems that cannot be ignored in the production of traditional fermented sausages in my country. For example, the fermented sausages produced by traditional farming methods have a long cycle, the source of microorganisms is complicated, the quality of the products is difficult to be guaranteed, and they are easily contaminated by bacteria.
In order to improve production efficiency, industrial production of sausage products often adopts rapid drying and maturation methods, which loses the unique flavor of fermented products.

Method used

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  • Lactobacillus fermentum cd110 and its application in the preparation of fermented sausage
  • Lactobacillus fermentum cd110 and its application in the preparation of fermented sausage
  • Lactobacillus fermentum cd110 and its application in the preparation of fermented sausage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the screening of bacterial strain

[0045] The specific implementation steps of the screening method are as follows:

[0046] 1. Sample collection

[0047] The original samples were collected from traditional naturally fermented sausages purchased from the vegetable market in Chengdu, Sichuan Province.

[0048] 2. Isolation of lactic acid bacteria from samples

[0049] Cut 25g sausage sample into pieces under aseptic conditions, dissolve in 225mL sterile normal saline, mix well, let it stand for a few minutes, after the minced meat is completely precipitated, take the supernatant and gradually dilute it by 1:10, choose the appropriate dilution 100ul of the bacterial solution was evenly spread on the MRS solid medium added with calcium carbonate, and cultured in an anaerobic incubator at 37°C for 48h. Pick independent single colonies with different shapes, calcium-dissolving circles, and conforming to the morphological characteristics of lactic acid bact...

Embodiment 2

[0061] Embodiment 2: Bile salt tolerance test of Lactobacillus fermentum CD110

[0062] Adjust the bacterial suspension of the strain to be tested to 10 9 CFU / mL. According to the ratio of 1% (V / V), they were respectively inoculated into MRS broth containing 0.1% and 0.3% bile salts, and the control group was composed of MRS broth without bile salts. Incubate anaerobically at 37°C for 24 hours, and measure OD620 by a spectrophotometer at 0.5h intervals. The bile salt tolerance of each strain was based on the time required to increase the OD620 value by 0.3 units.

[0063] The results are shown in Table 2. The time required for CD110 to increase OD620 by 0.3 units in 0.1% bile salts and 0.3% bile salts was significantly lower than that of the other two strains, indicating that CD110 has the strongest tolerance to bile salts.

[0064] Table 2 Bile salt tolerance results of different strains

[0065]

[0066] Note: The results are expressed as mean ± standard deviation, n=...

Embodiment 3

[0067] Embodiment 3: Lactobacillus fermentum CD110 gastric juice resistance test

[0068] Preparation of simulated gastric juice: 0.35 g of pepsin was added to 100 mL of 0.2% sterile saline, and the pH was adjusted to 3.0 with concentrated HCl, filtered and sterilized for later use.

[0069] The strain to be tested was cultured overnight in MRS broth at 37°C, and then inoculated into 5mL gastric juice at an amount of 1% and incubated at 37°C for 3 hours. After digestion in the simulated gastric juice, the number of viable bacteria (log10cfu / mL) of each bacterial strain was measured by plate counting method, and the survival rate was calculated, survival rate (%)=3h viable bacteria number / 0h viable bacteria number×100%.

[0070] The results are shown in Table 3. All three strains have good tolerance to gastric juice. After 3 hours of simulated digestion, the number of viable bacteria can still reach 10 7 cfu / ml, the survival rate of CD110 reached 98.98%, which was significantl...

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Abstract

The invention discloses a strain of lactobacillus fermentum CD110 and its application in preparing fermented sausage. The Lactobacillus fermentum (Lactobacillus fermentum) CD110 was deposited on July 17, 2018 in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.16123. The Lactobacillus fermentum CD110 is capable of producing protease, rapidly producing acid, not producing stickiness, not producing gas, not producing biogenic amines, and not producing H 2 o 2 , does not produce H 2 S, does not produce pigment, tolerates high salt concentration, high acidity, inhibits the growth of Escherichia coli and Staphylococcus aureus, is resistant to bile salts, and is resistant to gastric juice. When applied to fermented sausages, it can significantly increase the number of viable lactic acid bacteria in fermented sausages, reduce the pH value of sausages, inhibit the growth of harmful bacteria, and significantly improve the quality of fermented sausages.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a lactobacillus fermentum and its application in the preparation of fermented sausages. Background technique [0002] Fermented sausage refers to a fermented meat product made by mixing ground meat with animal fat, salt, sugar, starter and spices, etc., and pouring it into casings, and then undergoing microbial fermentation under artificial or natural control conditions. At this stage, there are some problems that cannot be ignored in the production of traditional fermented sausages in my country. For example, the fermented sausages produced by traditional farming methods have a long cycle, the source of microorganisms is complicated, the quality of the products is difficult to be guaranteed, and they are easily contaminated by bacteria. In order to improve production efficiency, industrial production of sausage products often adopts rapid drying and maturation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B4/22C12R1/225
CPCA23B4/22A23L13/45A23L13/65C12N1/20C12N1/205C12R2001/225
Inventor 孙健曹辰辰冯美琴徐幸莲周光宏
Owner NANJING AGRICULTURAL UNIVERSITY
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