Bacillus liquid compound microbial fertilizer and preparation method thereof
A technology of compound microorganisms and Bacillus, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of waste, insufficient utilization of nutrients, insufficient utilization of inorganic elements, etc. effect of quantity
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Embodiment 1
[0057] A kind of preparation of bacillus liquid composite microorganism bacterial fertilizer, its steps are as follows:
[0058] (1) Strain activation: transfer the Bacillus subtilis SEM-9 strain stored on a slope at 4°C to an LB medium plate, and culture at 35°C for 66 hours;
[0059] (2) Primary seed preparation: select a single colony with a fixed form on the plate, add it to a 250mL Erlenmeyer flask containing 75mL LB liquid medium, and cultivate it at a constant temperature for 17h at 35°C and 175rpm / min to obtain a primary seed solution ;
[0060] (3) Preparation of secondary seed liquid: the primary seed liquid obtained in step (2) is inserted into the seed tank at an inoculum size of 2.5%. : 1 ratio ventilation, tank pressure 0.065Mpa, cultivate 8.5h, obtain secondary seed liquid;
[0061] (4) Bacillus subtilis SEM-9 fermentation broth preparation:
[0062] 1) Fermentation broth preparation: adopt the following fermentation formula: silkworm excrement 50g / L, soybean...
Embodiment 2
[0069] Others are the same as in Example 1, except that: in step (4) of 1), the content of silkworm excrement is 10g / L; obtain bacillus liquid composite microbial fertilizer 2.
Embodiment 3
[0071] Others are the same as in Example 1, except that: in step (4) of 1), the content of silkworm excrement is 30g / L; obtain Bacillus liquid composite microbial bacterial fertilizer 3.
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