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Bacillus liquid compound microbial fertilizer and preparation method thereof

A technology of compound microorganisms and Bacillus, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of waste, insufficient utilization of nutrients, insufficient utilization of inorganic elements, etc. effect of quantity

Active Publication Date: 2019-02-12
GUANGDONG GEOLONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Silkworm feces, mulberry leaf residues and bedding materials in silkworm seats are collectively referred to as silkworm excrement, which is the main by-product of the silkworm business industry. Because of its rich content of crude protein, carbohydrates and other organic nutrients and inorganic elements such as phosphorus and potassium, it is the base fertilizer for crops. First choice, but directly using silkworm excrement as base fertilizer will lead to insufficient utilization of its nutrients, such as insufficient utilization of inorganic elements such as phosphorus and potassium, resulting in waste

Method used

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  • Bacillus liquid compound microbial fertilizer and preparation method thereof
  • Bacillus liquid compound microbial fertilizer and preparation method thereof
  • Bacillus liquid compound microbial fertilizer and preparation method thereof

Examples

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Effect test

Embodiment 1

[0057] A kind of preparation of bacillus liquid composite microorganism bacterial fertilizer, its steps are as follows:

[0058] (1) Strain activation: transfer the Bacillus subtilis SEM-9 strain stored on a slope at 4°C to an LB medium plate, and culture at 35°C for 66 hours;

[0059] (2) Primary seed preparation: select a single colony with a fixed form on the plate, add it to a 250mL Erlenmeyer flask containing 75mL LB liquid medium, and cultivate it at a constant temperature for 17h at 35°C and 175rpm / min to obtain a primary seed solution ;

[0060] (3) Preparation of secondary seed liquid: the primary seed liquid obtained in step (2) is inserted into the seed tank at an inoculum size of 2.5%. : 1 ratio ventilation, tank pressure 0.065Mpa, cultivate 8.5h, obtain secondary seed liquid;

[0061] (4) Bacillus subtilis SEM-9 fermentation broth preparation:

[0062] 1) Fermentation broth preparation: adopt the following fermentation formula: silkworm excrement 50g / L, soybean...

Embodiment 2

[0069] Others are the same as in Example 1, except that: in step (4) of 1), the content of silkworm excrement is 10g / L; obtain bacillus liquid composite microbial fertilizer 2.

Embodiment 3

[0071] Others are the same as in Example 1, except that: in step (4) of 1), the content of silkworm excrement is 30g / L; obtain Bacillus liquid composite microbial bacterial fertilizer 3.

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Abstract

The invention discloses bacillus liquid compound microbial fertilizer and a preparation method thereof, and belongs to the technical field of agricultural microbiology. The bacillus liquid compound microbial fertilizer is prepared from the following components in percentage by weight: 40-60% of Bacillus subtilis SEM-9 bacterial liquid, 20-40% of a nutrient complex, 1-3% of manganese sulfate, 4-8%of sodium methylene bis-naphthalene sulfonate and the balance of water. Bacillus subtilis SEM-9 bacterial strains in the bacillus liquid compound microbial fertilizer are derived from silkworm excrement, meanwhile through fermentation of the Bacillus subtilis SEM-9 bacterial strains, silkworm excrement liquid biologic fertilizer is produced, a growing environment approximating to the original ecology can be provided for the Bacillus subtilis SEM-9 bacterial strains to increase the number of live spores, and release of various nutrient substances in the silkworm excrement can also be better facilitated; and the compound microbial fertilizer is used for crops such as green cucumbers and cabbages, and can remarkably increase the yield of the crops and improve the resisting capacity to fusarium wilt.

Description

technical field [0001] The invention relates to a Bacillus subtilis liquid composite microbial fertilizer and a preparation method thereof, in particular to a Bacillus subtilis SEM-9 liquid composite microbial fertilizer and a preparation method thereof, belonging to the field of composite microbial fertilizers. Background technique [0002] Fusarium wilt is a worldwide soil-borne fungal disease of plants, and it is one of the main microbial diseases that cause crop yield reduction. Fusarium oxysporum Schl (Fusarium oxysporum Schl) is one of its most important pathogens, with a wide range of hosts. Chemical drugs are an effective and quick way to prevent and control Fusarium wilt, but the use of chemical drugs leads to soil degradation, damages the ecological balance, and even endangers human health. [0003] With social development and government guidance, sustainable ecological agriculture has become a mainstream trend, and breeding of disease-resistant varieties, crop ro...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C12N1/20A01C21/00C12R1/125C05G3/80
CPCA01C21/00C05B7/00C05G3/00C12N1/20C05G3/80C05C3/00C05C9/005C05C11/00C05D3/02C05D5/00C05D9/00C05D9/02C05F3/00C05F5/002C05F11/00C05F11/02C05F11/08C05G5/20Y02W30/40
Inventor 支胡钰李登勇代燕丽吴日章
Owner GUANGDONG GEOLONG BIOTECH
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