Method for hydrolyzing silkworm cocoons with weak alkali microwave

Abstract

The invention discloses a method for hydrolyzing silkworm cocoons with a weak base microwave, which comprises the following steps: (1) cleaning and drying; (2) cutting and separating; Add the mixed weak alkali mixture into a microwave reactor, hydrolyze it for 110-130min at 80°C under normal pressure, and obtain a crude sericin peptide solution and silk protein fiber solid after hydrolysis, extract the crude sericin peptide solution and perform ultrafiltration Filtrate to obtain sericin peptide protein; (4) Extract silk fibroin peptide protein by secondary hydrolysis, add weak alkali mixed solution into microwave reactor again, then hydrolyze at 100°C under normal pressure for 110-130min, and keep warm for 8- At 11 hours, the silk fibroin fiber solid is completely hydrolyzed into a thick silk fibroin solution, and the thick silk fibroin solution is subjected to ultrafiltration to obtain silk fibroin peptide protein. The invention can effectively prepare sericin peptide protein and silk fibroin peptide protein at one time, and achieves the purpose of improving preparation efficiency and product utilization rate.

Description

technical field [0001] The invention relates to the technical field of silk protein, in particular to a method for hydrolyzing silkworm cocoons with a weak alkali microwave. Background technique [0002] The silk industry that originated in my country has a long history of thousands of years. However, the application of silk is mainly to use silk fibroin as a clothing material. With the progress of society and the development of modern science and technology, the development and utilization of new functions of silk are becoming more and more important. more and more a trend. [0003] In the late 1920s, the silk industry experienced overproduction for the first time in the world. Since 1933, people have taken the lead in applying silk to non-clothing clothing field and through research and use, so far, the multi-functional development and utilization of silk protein has begun. The research found that silk is mainly composed of 20-30% sericin peptide protein and 70-80% silk fi...

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned patents can prepare sericin peptide protein or silk fibroin peptide protein well, but because sericin and silk fibroin complement each other, the above-mentioned patents can only prepare one kind of sericin peptide protein or silk fibroin peptide protein, and Can not prepare two kinds at the same time, it has defects such as low preparation efficiency and low product utilization rate

Moreover, both of the above two pieces used strong acid or strong base (sodium hydroxide) in the preparation process. After hydrolysis, it is necessary to neutralize the acid or base of the hydrolyzed liquid and physically extract the silk fibroin sericin peptide protein. Waste liquid treatment and discharge The amount is also increased, and the pollution control of the post-process is increased, and the efficiency of the process will be low if the process is increased.

Therefore, there are defects such as low preparation efficiency, high manufacturing cost, large pollution, and large protein damage, resulting in that the quality of the product cannot be guaranteed.