Quantum dot low-density lipoprotein nano-composite probe as well as preparation method and application

A low-density lipoprotein and nanocomposite technology, which is applied in the field of quantum dot low-density lipoprotein nanocomposite probe and preparation, can solve the problems of high cost, poor biocompatibility, complicated preparation, etc., and achieves strong loading capacity, coupling Fast, repeatable results

Active Publication Date: 2019-02-12
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The multifunctional nanoprobes studied in recent years (such as fluorescence-drug delivery, fluorescence-photothermal, etc.) have disadvantages such as poor biocompatibility, complicated preparation, and high cost, and cannot be well applied in the fields of biological detection, imaging, and diagnosis and treatment.

Method used

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  • Quantum dot low-density lipoprotein nano-composite probe as well as preparation method and application
  • Quantum dot low-density lipoprotein nano-composite probe as well as preparation method and application
  • Quantum dot low-density lipoprotein nano-composite probe as well as preparation method and application

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preparation example Construction

[0030] The method for preparing the above-mentioned composite probe according to one embodiment of the present invention can be composed of figure 1 shown schematically. The cationic polyelectrolyte was first mixed with LDL, and then mixed with sulfhydryl or carboxyl-modified quantum dots.

[0031] The method for preparing composite probes according to one embodiment of the present invention comprises the following steps:

[0032] 1) Prepare the dispersion liquid of cationic polyelectrolyte

[0033] Provide cationic polyelectrolyte, add deionized water to dilute, then add sodium chloride to adjust its concentration. Sodium hydroxide was then added to adjust its pH. Specifically, add cationic polyelectrolyte (including polydiallyldimethylammonium chloride or polyacrylamide of any molecular weight) into deionized water, dilute to a concentration of 0.005-0.01mol / L, add sodium chloride solution , so that the concentration of sodium chloride is 0.05-1mol / L, add sodium hydroxid...

Embodiment 1

[0041] 1) Add cationic polyelectrolyte (polydiallyl dimethyl ammonium chloride of any molecular weight) into deionized water, dilute to a concentration of 0.05mol / L, add sodium chloride solution, so that the concentration of sodium chloride is 0.05mol / L, add sodium hydroxide solution to adjust the pH of the mixture to 7.2.

[0042] 2) Add quantum dot powder to phosphate buffer (sodium phosphate buffer), and adjust the concentration of quantum dots to 1 μmol / L.

[0043] 3) Add human low-density lipoprotein powder into phosphate buffer (sodium phosphate buffer), add sodium hydroxide solution, and adjust the pH of the mixture to 7.2.

[0044] 4) Take 10 mL of human low-density lipoprotein dispersion, add 10 mL of cationic polyelectrolyte solution dropwise, and stir evenly. Then add 1mL of quantum dot dispersion liquid with a peristaltic pump, mix evenly, and then centrifuge and wash to obtain a quantum dot low-density lipoprotein nanocomposite probe.

Embodiment 2

[0046] 1) Add cationic polyelectrolyte (polydiallyl dimethyl ammonium chloride of any molecular weight) into deionized water, dilute to a concentration of 0.07mol / L, add sodium chloride solution, so that the concentration of sodium chloride is 0.07mol / L, add sodium hydroxide solution to adjust the pH of the mixture to 7.2.

[0047] 2) Add quantum dot powder to phosphate buffer (sodium phosphate buffer), and adjust the concentration of quantum dots to 1 μmol / L.

[0048] 3) Add human low-density lipoprotein powder into phosphate buffer (sodium phosphate buffer), add sodium hydroxide solution, and adjust the pH of the mixture to 7.2.

[0049] 4) Take 10 mL of human low-density lipoprotein dispersion, add 10 mL of cationic polyelectrolyte solution dropwise, and stir evenly. Then add 1mL of quantum dot dispersion liquid with a peristaltic pump, mix evenly, and then centrifuge and wash to obtain a quantum dot low-density lipoprotein nanocomposite probe.

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Abstract

The invention provides a quantum dot low-density lipoprotein nano-composite probe as well as a preparation method and application. The quantum dot low-density lipoprotein nano-composite probe is prepared from low-density lipoprotein, cationic polyelectrolyte and water-soluble quantum dots; the low-density lipoprotein is the core; and the water-soluble quantum dots are coupled to the low-density lipoprotein through the cationic polyelectrolyte. The low-density lipoprotein and the water-soluble quantum dots are assembled by adopting an electrostatic grafting method to form the fluorescent probe,and the probe has the very strong chemical stability and biocompatibility, and can provide the excellent spectral resolution.

Description

technical field [0001] The invention relates to the field of biological materials, and relates to a quantum dot low-density lipoprotein nanocomposite probe, a preparation method and an application. Background technique [0002] Quantum dots, also known as semiconductor nanocrystals, are materials with a size of 1 to 20 nm composed of a small number of atoms. Due to the quantum confinement effect and size effect, quantum dots will emit fluorescence of different wavelengths after being excited by external light. Compared with traditional organic fluorescent dyes, quantum dots have great advantages due to their simple preparation, narrow fluorescence peak and strong photochemical stability. Low-density lipoprotein is a native lipoprotein with a diameter of about 20-40nm, containing a monolayer of amphiphilic phospholipids and a hydrophobic core rich in cholesteryl esters. Due to its small size, high loading capacity and natural tumor targeting ability, it has broad applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02A61K49/00A61K47/64A61K47/42G01N21/64
CPCA61K47/42A61K49/0056A61K49/0067A61K47/64C09K11/025G01N21/6428
Inventor 胡锦航宋江鲁奇唐志书朱立新周慧鑫许小亮宋忠兴
Owner SHAANXI UNIV OF CHINESE MEDICINE
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