Unlock instant, AI-driven research and patent intelligence for your innovation.

Hepatitis B DNA detection kit containing specific PCR buffer solution

A detection kit and buffer technology, which is applied in the field of hepatitis B DNA detection kits, can solve problems such as easy confusion, achieve the effects of less error-prone, rapid mass configuration, and improved detection accuracy

Inactive Publication Date: 2019-02-12
上海裕隆神光医学检验实验室有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the detection of HBV DNA, the PCR process requires a large number of reaction systems, which is very easy to confuse and has a great impact on experimental workers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hepatitis B DNA detection kit containing specific PCR buffer solution
  • Hepatitis B DNA detection kit containing specific PCR buffer solution
  • Hepatitis B DNA detection kit containing specific PCR buffer solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of hepatitis B DNA detection kit containing specific PCR buffer

[0029] like Figure 1-Figure 7 As shown, the detection kit includes a box body 1, a reagent bottle 2, a fixed frame 3, a shockproof sponge block 4, and a PCR plate 5; the fixed frame 3 includes a top surface, a bottom surface and two side walls, and between the top surface and the bottom surface A partition 6 is provided, and the partition 6 divides the fixed frame into A part 7 and B part 8. The top surface of the fixed frame 3 is provided with a fixed hole 301, and the anti-vibration sponge block 4 is provided with a concave hole matched with the fixed hole 301. Groove 401, the anti-shock sponge 4 is arranged on the A part 7 of the fixed frame 3, and the top surface of the anti-shock sponge 4 fits the inner side of the top surface of the fixed frame 3, the reagent bottle 2 is placed in the groove 401, and the PCR plate 5 is placed on the fixed frame Part B of 3 5;

[0030] The PCR ...

Embodiment 2

[0032] Embodiment 2 A kind of hepatitis B DNA detection kit containing specific PCR buffer

[0033] On the basis of the kit structure in Example 1, such as Figure 1-Figure 7 As shown, the horizontal mark block 503 is provided with a triangular mark 512;

[0034] The PCR buffer consists of the following components: trimethylglycine 4.5g / L, ammonium sulfate 5.5mM, trehalose 1.5g / L, calcium chloride 1.5g / L, Triton X-1000 3% (v / v ), sodium azide 0.2g / L, and deionized water as the balance; use sodium hydroxide to adjust the pH of the PCR buffer to 9.1.

Embodiment 3

[0035] Embodiment 3 A kind of hepatitis B DNA detection kit containing specific PCR buffer

[0036] On the basis of the kit structure in Example 2, such as Figure 1-Figure 7 As shown, the horizontal marking block 503 is a cube, and the triangular marking 512 is located on one side of the horizontal marking block 503;

[0037] The PCR buffer is composed of the following components: trimethylglycine 4.6g / L, ammonium sulfate 5.3mM, trehalose 2g / L, calcium chloride 2.5g / L, Triton X-1000 4% (v / v) 1. Sodium azide 0.1g / L, the balance is deionized water; use sodium hydroxide to adjust the pH of the PCR buffer to 9.4.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a hepatitis B DNA detection kit containing a specific PCR buffer solution and belongs to the technical field of biological technology devices. The detection kit provided by the invention not only relates to a structure of the kit, but also relates to the PCR buffer solution used by the kit. The detection kit comprises a box body, a reagent bottle, a fixed frame, a shakeproof sponge block and a PCR board; a plurality of PCR pipe accommodating grooves are arranged in single-low boards in the PCR board; a first horizontal rod penetrates through a punched hole A, a secondhorizontal rod penetrates through a punched hole B, and the plurality of single-row boards can be arranged on a board frame; a first longitudinal rod penetrates through a punched hole C to arrange a horizontal tag block on the board frame; the PCR buffer solution is prepared from trimethylglycine, ammonium sulfate, trehalose, calcium chloride, Triton X-1000, sodium azide and deionized water. Whenthe hepatitis B DNA detection kit disclosed by the invention is utilized, a lot of PCR reaction systems can be quickly configured; furthermore, errors are not prone to occurring; meanwhile, the PCR buffer solution disclosed by the invention has strong stability and is not prone to crystallizing, so that hepatitis B detection flexibility is improved.

Description

technical field [0001] The invention relates to a hepatitis B DNA detection kit containing a specific PCR buffer, belonging to the technical field of biotechnology devices. Background technique [0002] Hepatitis B virus (hepatitis B virus, HBV) is the pathogen that causes hepatitis B (hepatitis B for short), and belongs to the family Hepadnaviridae, which includes two genera of Orthohepadnavirus and Avian Hepadnavirus , causing human infection is positive hepadnavirus genus. [0003] HBV DNA detection, through the amplification of viral nucleic acid, is sensitive to low levels of HBV virus in the body, and is a common method for judging virus replication. DNA testing also plays a very important role in diagnosing HBV and evaluating the effect of HBV treatment. It can be used as an evaluation index to understand the number of viruses in the body, replication level, infectivity, drug treatment effect, and formulate treatment strategies. It is also the only one that can help ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/686
CPCC12Q1/686C12Q1/706
Inventor 穆海东汪宁梅
Owner 上海裕隆神光医学检验实验室有限公司