Hemostyptic microsphere containing antibacterial ingredient berberine as well as preparation method and application of hemostyptic microsphere

An antibacterial ingredient, berberine technology, applied in hemostatic microspheres containing antibacterial ingredient berberine and its preparation and application fields, can solve the problems of being difficult to degrade and absorb, and not suitable for hemostatic powder, so as to achieve convenient use and shorten hemostasis time , Improve the effect of adhesion

Active Publication Date: 2019-02-15
高莱(上海)医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of material hopes to use halloysite and chitosan to play the respective advantages of inorganic and organic materials to play a hemostatic and bactericidal effect, but as it says, halloysite nanotubes are inorganic materials, and the main components are silicon oxide, aluminum oxide and a small amount of other metals Oxides, such ingredients are difficult to be degraded and absorbed in the body, and cannot be hydrolyzed by lysozyme to exert a synergistic effect, so it is not suitable as a hemostatic powder for hemostasis in vivo

Method used

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  • Hemostyptic microsphere containing antibacterial ingredient berberine as well as preparation method and application of hemostyptic microsphere
  • Hemostyptic microsphere containing antibacterial ingredient berberine as well as preparation method and application of hemostyptic microsphere
  • Hemostyptic microsphere containing antibacterial ingredient berberine as well as preparation method and application of hemostyptic microsphere

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of matrix solution: Add 15 g of sodium alginate, 10 g of sodium carboxymethylcellulose and 0.25 g of gelatin to 1000 ml, 500 ml, and 500 ml of water and stir to dissolve, then mix the three solutions to make a matrix solution .

[0039] 2. Material mixing and emulsification cross-linking copolymerization: 2000 ml ddH 2 O was added to the beaker, and then 187.5 ml of Tween 80 was added, stirred rapidly for about 5 minutes, then the matrix solution was added, and emulsified and stirred for 30 minutes. After emulsification, slowly add 2% CaCl dropwise 2 Solution 10 ml, for cross-linking reaction, after dropwise stirring for 1 h. After the cross-linking is completed, add 5000ml of absolute ethanol to soak for 2 h.

[0040] 3. Refining: After the solution is separated, discard the lower layer solution, filter with suction, wash the crude sample 3 times with 1000 ml absolute ethanol, and then wash 3 times with 1000 ml petroleum ether. The eluted sample was ...

Embodiment 2

[0042] 1. Preparation of matrix solution: Add 15 g of sodium alginate, 10 g of sodium carboxymethylcellulose and 0.25 g of gelatin to 1000 ml, 500 ml, and 500 ml of water and stir to dissolve, then mix the three solutions to make a matrix solution .

[0043] 2. Material mixing and emulsification cross-linking copolymerization: 2000 ml ddH 2 O was added to the beaker, and then 187.5 ml of Tween 80 was added, stirred rapidly for about 5 minutes, then the matrix solution was added, and emulsified and stirred for 30 minutes. After emulsification, slowly add 2% CaCl dropwise 2 Solution 10 ml, for cross-linking reaction, after dropwise stirring for 1 h. After the cross-linking is completed, add 5000ml of absolute ethanol to soak for 2 h.

[0044] 3. Refining: After the solution is separated, discard the lower layer solution, filter with suction, wash the crude sample 3 times with 1000 ml absolute ethanol, and then wash 3 times with 1000 ml petroleum ether. Dissolve the eluted sa...

Embodiment 3

[0046] 1. Preparation of matrix solution: Add 15 g of sodium alginate, 10 g of sodium carboxymethylcellulose and 0.25 g of gelatin to 1000 ml, 500 ml, and 500 ml of water and stir to dissolve, then mix the three solutions to make a matrix solution .

[0047] 2. Material mixing and emulsification cross-linking copolymerization: 2000 ml ddH 2 O was added to the beaker, and then 187.5 ml of Tween 80 was added, stirred rapidly for about 5 minutes, then the matrix solution was added, and emulsified and stirred for 30 minutes. After emulsification, slowly add 2% CaCl dropwise 2 Solution 10 ml, for cross-linking reaction, after dropwise stirring for 1 h. After the cross-linking is completed, add 5000ml of absolute ethanol to soak for 2 h.

[0048] 3. Refining: After the solution is separated, discard the lower layer solution, filter with suction, wash the crude sample 3 times with 1000 ml absolute ethanol, and then wash 3 times with 1000 ml petroleum ether. The eluted sample was ...

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Abstract

The application relates to a hemostyptic microsphere containing an antibacterial ingredient berberine as well as a preparation method and application of the hemostyptic microsphere, and a hemostatic agent can be used in human bodies and can be quickly degraded and absorbed. According to the invention, a composite antibacterial hemostyptic microsphere material taking sodium alginate, sodium carboxymethylcellulose, gelatin and the berberine as raw materials is adopted, the characteristics of bacteria resistance and adhesion of the berberine are utilized, so that the material is endowed with an antibacterial property, and a hemostyptic effect of the material is further enhanced. Meanwhile, in a simulated body fluid environment after hemostasis, hemostyptic antibacterial effective ingredientsincluding the sodium alginate, the sodium carboxymethylcellulose, the gelatin and the berberine are degraded, so that an effect that the hemostyptic material can be quickly degraded and absorbed in human bodies is achieved.

Description

technical field [0001] The invention relates to a hemostatic microsphere containing antibacterial component berberine, a preparation method and related applications thereof. Background of the invention [0002] Massive wound bleeding has always been the main cause of death in patients' operations and wars. Rapid and effective hemostasis can save patients' lives at critical moments, which is of great clinical significance. In recent years, in response to the demand for rapid hemostasis, various hemostatic materials have appeared on the market, mainly including zeolite dressings, chitosan dressings, gelatin sponge, oxidized regenerated cellulose, etc. Adhesive wounds form a hemostatic microenvironment. Although gelatin sponge can quickly absorb blood and swell, and has a good hemostasis effect, it should be used with caution in places where neurons are dense, such as the nervous system, otherwise it will compress the nerves and cause immeasurable harm. Oxidized regenerated c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/08A61L24/00
CPCA61L24/00A61L24/0005A61L24/0015A61L24/0042A61L24/08A61L24/104A61L2300/204A61L2300/404A61L2300/412A61L2300/418A61L2400/04C08L5/04C08L1/286
Inventor 叶飞金甲代凯丽王林张伦
Owner 高莱(上海)医药科技有限公司
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