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InDel6 and SSR229 markers closely linked to height of maize plant and application of InDel6 and SSR229 markers

A marker and corn technology, applied in the fields of genetic engineering and molecular biology, to achieve stable performance, strong specificity, and improved efficiency

Active Publication Date: 2019-02-15
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some genes regulating maize plant height have been mapped and cloned, such as qPH3.1, br2, etc., there is no research report on the use of InDel6 and SSR229 molecular markers for maize plant height-assisted selection breeding

Method used

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  • InDel6 and SSR229 markers closely linked to height of maize plant and application of InDel6 and SSR229 markers
  • InDel6 and SSR229 markers closely linked to height of maize plant and application of InDel6 and SSR229 markers
  • InDel6 and SSR229 markers closely linked to height of maize plant and application of InDel6 and SSR229 markers

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Construction of gene mapping population

[0034] 1. Construction of BC groups

[0035] Maize inbred line Mo17 (female parent) is a backbone inbred line for corn breeding practice and basic research. It belongs to maize Lancaster germplasm, not Reid group, and the plant height is normal, about 170cm. The genome of inbred line Mo17 has been sequenced.

[0036] The corn dwarf material DNT06 (male parent) has a dwarf plant height of about 100 cm and normal pollen fertility. The dwarf traits of the dwarf material DNT06 have been identified in multiple years and locations (Yangzhou, Sanya, Beijing), and the dwarf traits are stable and not affected by years and environments.

[0037] Implementation cases of the present invention utilize these two parental configurations F 1 , F 1 The offspring were backcrossed with the dwarf material DNT06 to produce BC 1 generation. Then, in the backcross progeny group, select plants with normal height as the female parent, and the dw...

Embodiment 2

[0050] Detection of Multiple Tall and Dwarf Materials Using Plant Height Tightly Linked InDel6 and SSR229 Markers

[0051] (1) Select 58 high-stalk materials and 62 short-stalk materials.

[0052] (2) Genomic DNA was extracted from 120 materials respectively, and PCR amplification and polyacrylamide gel electrophoresis detection were performed using InDel6 and SSR229 markers. The results of InDel6 in 58 high-stem materials and 62 short-stalk materials showed that the genotype was consistent with the phenotype (such as Figure 6 ). The results of SSR229 in 58 high-stalk materials and 62 short-stalk materials showed that the genotype was consistent with the phenotype (such as Figure 7 ).

[0053] (3) The above two InDel6 and SSR229 markers can distinguish tall stalk material from dwarf stalk material.

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Abstract

The invention discloses InDel6 and SSR229 markers closely linked to the height of a maize plant and application of the InDel6 and SSR229 markers. Labeled nucleotide sequences are: a forward primer 5'-TGTGGACGTGACACGCTT-3' and a reverse primer 5'-TCAGCCTTCGTCCACAGAC-3' of InDel6, and a forward primer 5'-GGGTCTTTCGCTGAATAACACT-3' and a reverse primer 5'-CAACCCTCACTACTAAAGCCGA-3' of SSR229. The invention also discloses the application of the markers in the high molecular marker-assisted selection breeding of corn plants. A specific method comprises the steps as follows: (1) performing genotype identification on BC and F2 population strains by molecular markers; (2) classifying high stalks and dwarf stalks according to results of the genotype identification in the step (1), and performing verification on genotype identification by combining phenotypic results. The molecular markers provided the invention are stable in performance, simple to detect, clear in electrophoresis bands and low inexperimental cost, have no need for enzymatic cleavage, improve the efficiency of maize plant type improvement, and accelerate the process of ideal plant type breeding of corn.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a molecular marker closely linked with the plant height of maize and its application. Background technique [0002] Maize (Zea mays L.) is the largest food crop in my country and plays a pivotal role in China's energy and food security. Plant height affects the lodging resistance, photosynthetic efficiency and harvest index of maize, and is closely related to maize yield. , in the practice of maize breeding and the improvement of germplasm resources, the trait of plant height has important value. [0003] Maize plant height has a complex genetic basis. Methods such as linkage mapping and association analysis have been used to study maize plant height. On the one hand, linkage mapping and association analysis methods are used to discover targets that affect plant height. On the other hand, the dwarf mutant was used to analyze the mechanism of plant height ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 王益军王亚丽周波赵佳邓德祥
Owner YANGZHOU UNIV
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