Preparation of chitosan-covering zein nanoparticles and application thereof in load acacetin sodium salt

A technology of zein and chitosan, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, and drug combinations, etc., can solve the problems of poor chemical stability of acacetin sodium salt and the like , to achieve the effects of excellent stability, high drug encapsulation efficiency, and high nanoparticle yield.

Inactive Publication Date: 2019-02-19
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical stability of acacetin sodium salt is not good, and it is sensitive to high humidity and high temperature. Encapsulating it in nanoparticles can improve its stability

Method used

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  • Preparation of chitosan-covering zein nanoparticles and application thereof in load acacetin sodium salt
  • Preparation of chitosan-covering zein nanoparticles and application thereof in load acacetin sodium salt
  • Preparation of chitosan-covering zein nanoparticles and application thereof in load acacetin sodium salt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Precisely weigh 100.1 mg of zein and dissolve it in 10 ml of 80% ethanol, ultrasonicate for 5 minutes to dissolve it completely, and place it in a 50 ml spray bottle;

[0036] (2) Measure 10ml of deionized water and place it in another 50ml spray bottle;

[0037] (3) At room temperature, spray two spray bottles in a closed device to obtain a milky white solution. The resulting solution was rotary evaporated at 50°C for 30 min to remove ethanol;

[0038] (4) Get the supernatant as a sample by centrifuging at 2000rpm for 10min, measure the particle size and potential of the product with nano-zs90 Malvern particle size and potential meter, the average particle size is about 180.5nm (polydispersity index=0.209), and the potential is -11.9mv, the particle size and potential distribution of nanoparticles are shown in the attached figure 1 .

Embodiment 2

[0040] (1) Precisely weigh 100.3 mg of zein, dissolve it in 10 ml of 80% ethanol, ultrasonicate for 5 minutes to dissolve it completely, and place it in a 50 ml spray bottle;

[0041] (2) Precisely weigh 5.2mg PEG400 and dissolve it in 10ml deionized water, sonicate for 5 minutes to dissolve it completely, and place it in another 50ml spray bottle;

[0042] (3) At room temperature, spray two spray bottles in a closed device to obtain a milky white solution. The resulting solution was rotary evaporated at 50°C for 30 min to remove ethanol;

[0043] (4) The supernatant was taken as a sample by centrifugation at 2000 rpm for 10 min, and the particle size of the product was measured with a nano-zs90 Malvern particle size and potentiometer. The average particle size of the zein nanoparticles stabilized by 5% PEG400 is about 159.3nm (polydispersity index=0.186), and the particle size distribution diagram is shown in figure 2 .

Embodiment 3

[0045] (1) Precisely weigh 100.3 mg of zein, dissolve it in 10 ml of 80% ethanol, ultrasonicate for 5 minutes to dissolve it completely, and place it in a 50 ml spray bottle;

[0046] (2) Precisely weigh 5.3mg of F68 and dissolve it in 10ml of deionized water, sonicate for 5 minutes to dissolve it completely, and place it in another 50ml spray bottle;

[0047] (3) At room temperature, spray two spray bottles in a closed device to obtain a milky white solution. The resulting solution was rotary evaporated at 50°C for 30 min to remove ethanol;

[0048] (4) Take the supernatant as a sample by centrifuging at 2000rpm for 10min, measure the particle diameter of the product with a nano-zs90 Malvern particle diameter and potentiometer, and the particle diameter of the zein nanoparticles stabilized by 5% F68 is about 246.7nm (polydispersity index=0.224), particle size distribution figure sees image 3 .

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Abstract

The invention discloses a preparing method and application of chitosan-covering zein nanoparticles. The preparing method of the nanoparticles comprises the steps that an ethanol solution of zein is added into a spraying bottle with the droplet particle size of about 10-50 microns, a water phase is added into another spraying bottle with the droplet particle size of about 10-50 microns, and the twospraying bottles conduct opposite spraying in a closed device to obtain a solution containing the zein nanoparticles, wherein for the zein nanoparticles, the particle size is about 160-200 nm, and the zeta potential is minus 8 to 12 mv. A proper amount of a zein nanoparticle solution is added into a chitosan solution, through incubation, the chitosan-covering zein nanoparticles can then be obtained, and for the chitosan-covering zein nanoparticles, the particle size is 160-180 nm, and the zeta potential is about 50 mv. According to the preparing method and application of the chitosan-coveringzein nanoparticles, the chitosan-covering zein nanoparticles can be continuously produced, and the preparing method is simple, free of pollution, mild, safe and high in yield. When the chitosan-covering zein nanoparticles are used for loading acacetin sodium salt, the drug loading capacity is about 20-30%, the particle size is 190-260 nm, the zeta potential is 35-45 mv, and the stability of the acacetin sodium salt can be improved after the acacetin sodium salt is covered with the nanoparticles.

Description

technical field [0001] The invention relates to the technical field of biomedical nano-sustained release, in particular to a preparation method of chitosan-wrapped zein nano-particles and the application of loaded acacetin sodium salt. Background technique [0002] Zein is the main storage protein in corn, accounting for 35-60% of the total endosperm protein, with an average molecular weight of about 40kD. Since zein contains a large amount of hydrophobic amino acids, such as glutamine and proline; it lacks chargeable acidic and basic amino acids, especially tryptophan and lysine; it contains more sulfur-containing amino acids, Due to its unbalanced amino acid composition, its nutritional value is not high. In recent years, studies have found that the hydrophilic part and hydrophobic part in the molecular structure of zein are clearly divided, and have unique self-assembly properties, film-forming properties, gel properties, water resistance, oil resistance, and good biocom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K47/36A61K31/352A61P29/00A61P35/00A61P39/06A61P9/06
CPCA61K9/5161A61K9/5169A61K31/352A61P9/06A61P29/00A61P35/00A61P39/06
Inventor 王亚晶葛铭晨周舒文
Owner CHANGZHOU UNIV
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