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Sialic acid and extraction method thereof

An extraction method, a technology of sialic acid, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of complex process, high cost, and many operation steps, and achieve the effect of simple operation and short working hours

Active Publication Date: 2019-02-22
武汉中科光谷绿色生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process is complicated, with many operation steps, high cost, and long hours of separation and purification, which is obviously not applicable in actual scale production

Method used

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  • Sialic acid and extraction method thereof
  • Sialic acid and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Use a 50nm ceramic membrane to filter the 10g / L polysialic acid fermentation broth, and then use an 8KDa ultrafiltration membrane to filter until the solid content of the solution is less than 3 times the polysialic acid content to obtain a polysialic acid solution. Add hydrochloric acid to the polysialic acid solution until the pH of the solution is 1, and react at a temperature of 80° C. for 4 hours to obtain a sialic acid solution. Adding to the sialic acid solution the ratio of 0.5% activated carbon to the sialic acid solution mass for decolorization treatment to obtain a decolorization solution, adding sodium hydroxide to the pH of the solution is 5 and obtains a sialate solution, and adopts a 0.5KDa ultrafiltration membrane to decolorize the solution The incompletely hydrolyzed polysialic acid was removed by filtration. A 200Da nanofiltration membrane is used to desalt the sialate solution, so that the solid content in the nanofiltration solution is less than three...

Embodiment 2

[0036] A 100nm ceramic membrane was used to filter the 15g / L polysialic acid fermentation broth, and then a 9KDa ultrafiltration membrane was used to filter until the solid content of the solution was less than 3 times the polysialic acid content to obtain a polysialic acid solution. Add hydrochloric acid to the polysialic acid solution until the pH of the solution is 1, and react at a temperature of 85° C. for 3 hours to obtain a sialic acid solution. Adding to the sialic acid solution the ratio of the mass of the sialic acid solution to the sialic acid solution is 1.5% activated carbon decolorization treatment to obtain a decolorization solution, adding sodium hydroxide to the pH of the solution is 5 and obtains a sialate solution, and adopts a 0.8KDa ultrafiltration membrane to decolorize the solution The incompletely hydrolyzed polysialic acid was removed by filtration. A 250Da nanofiltration membrane is used to desalt the sialate solution to obtain a nanofiltration soluti...

Embodiment 3

[0038] A 150nm ceramic membrane is used to filter the 20g / L polysialic acid fermentation broth, and then a 10KDa ultrafiltration membrane is used to filter until the solid content of the solution is less than 3 times the polysialic acid content to obtain a polysialic acid solution. Sulfuric acid was added to the polysialic acid solution until the pH of the solution was 2, and the reaction was carried out at a temperature of 90° C. for 2 hours to obtain a sialic acid solution. Adding to the sialic acid solution the ratio of the mass of the sialic acid solution to 2.5% gac decolorization treatment to obtain a decolorization solution, adding sodium hydroxide to the pH of the solution is 6 and obtains a sialate solution, and adopts an ultrafiltration membrane of 1.0KDa to decolorize the solution The incompletely hydrolyzed polysialic acid was removed by filtration. A 300Da nanofiltration membrane is used to filter the sialate solution to obtain a nanofiltration solution, so that t...

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Abstract

The invention discloses sialic acid and an extraction method thereof, and belongs to the technical field of sialic acid extraction. Sialic acid solution obtained by pretreating a sialic acid monomer source is subjected to alkaline neutralization, concentration purification, heating concentration, acidification and crystallization to obtain the sialic acid. The extraction method of the sialic acidhas the advantages of short working time, low cost and high yield and purity of the extracted sialic acid.

Description

technical field [0001] The invention belongs to the technical field of sialic acid extraction, and in particular relates to a sialic acid and an extraction method thereof. Background technique [0002] The preparation methods of sialic acid mainly include chemical synthesis, enzyme synthesis, natural product extraction and microbial fermentation. The microbial method obtains polysialic acid through fermentation, and then hydrolyzes the polysialic acid to obtain sialic acid monomers. [0003] The method commonly used in the prior art is to take the supernatant after the centrifugation of the fermented liquid, add ethanol or complexing agent to remove protein precipitation, and purify by ion exchange column or the mode of passing membrane, obtain polysialic acid, polysialic acid (PSA) Then go through the steps of acid hydrolysis and decolorization, and finally crystallize with ethyl acetate or glacial acetic acid. The entire process is complex, with many steps, high cost, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H7/033C07H1/00
CPCC07H1/00C07H1/06C07H7/033
Inventor 李翔宇陆姝欢张力文马凡提刘宏荣陈祥松王煜
Owner 武汉中科光谷绿色生物技术有限公司
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