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Chitosan-based hydrogel, and preparation method and application thereof

A technology of chitosan and hydrogel, which is applied in the field of medical materials, can solve problems such as limited applications, and achieve the effect of promoting wound healing, good biocompatibility, and excellent biocompatibility

Inactive Publication Date: 2019-02-26
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reported mussel silk protein adhesives need to be cured at a certain temperature for a long time before they can achieve high adhesive strength, which limits their clinical application.

Method used

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  • Chitosan-based hydrogel, and preparation method and application thereof
  • Chitosan-based hydrogel, and preparation method and application thereof
  • Chitosan-based hydrogel, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Hydrogel 1

[0028] Weigh 0.5 g of chitosan with a deacetylation degree of 90% and an apparent molecular weight of 400 kDa in a round-bottomed flask, add 50 mL of phosphate buffer solution with a pH of 6.5, and stir thoroughly to dissolve it completely. Weigh 0.43 g EDC and 0.17 g NHS and put them in a beaker, mix them with 25 mL of ethanol solution containing 0.13 g 3,4-dihydroxyphenylpropionic acid, add this mixture into the chitosan solution, and stir the reaction rapidly for 24 h, after the reaction, the mixture was first dialyzed in HCl solution with pH 5.0 for 2 days. Finally, the product is freeze-dried to obtain white spongy grafted phthalo-dihydroxy chitosan. After irradiation sterilization, the grafted o-phthalic dihydroxy chitosan was stirred and dissolved in a sterile phosphate buffer solution with a pH of 6.5 to a concentration of 13% by weight, and a concentration of 3.25 mg / mL period iodine was added. Sodium acid sterile aqueous solution, so ...

Embodiment 2

[0029] Example 2: Hydrogel 2

[0030] Weigh 0.5 g of hydroxyethyl chitosan and place it in a round bottom flask, add 50 mL of phosphate buffer solution with a pH of 6.5, stir well to dissolve it completely. Weigh 0.55 g EDC and 0.21 g NHS and place them in a beaker, mix them with 25 mL of ethanol solution containing 0.17 g 3,4-dihydroxyphenylpropionic acid, add this mixture to the hydroxyethyl chitosan solution, and quickly The reaction was stirred for 24 h. After the reaction, the mixture was dialyzed in HCl solution with pH 5.0 for 2 days. Finally, the product was freeze-dried to obtain white spongy grafted phthalic dihydroxy hydroxyethyl chitosan, wherein the grafting rate of phthalic dihydroxy groups was 14%. Stir and dissolve the grafted o-phthalic dihydroxy hydroxyethyl chitosan after irradiation sterilization in a sterile phosphate buffer solution with a pH of 6.5 to make its weight percent concentration 13%, and add a concentration of 3.25% under stirring conditions. ...

Embodiment 3

[0031] Example 3: Hydrogel 3

[0032] Weigh 0.5 g of hydroxypropyl chitosan and place it in a round bottom flask, add 50 mL of phosphate buffer solution with a pH of 6.5, stir well to dissolve it completely. Weigh 0.59 g of EDC and 0.22 g of NHS and mix with 25 mL of 3,4-dihydroxyphenylacetic acid ethanol solution containing 0.16 g, add this mixture into the hydroxypropyl chitosan solution, and stir rapidly for 24 h. After the end, the mixed solution was dialyzed, and then freeze-dried to obtain a white spongy grafted o-phthalic dihydroxy hydroxypropyl chitosan solid. After irradiation sterilization, the grafted o-phenylenedihydroxypropyl chitosan was stirred and dissolved in a sterile phosphate buffer solution with a pH of 6.5, so that the concentration by weight was 13%, and the added concentration was 3.25 mg / mL Sodium periodate sterile aqueous solution, make the molar ratio of the grafted o-phthalic dihydroxy group and sodium periodate be 1:0.25, mix well, constitute the ...

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Abstract

The invention discloses a chitosan-based hydrogel, and a preparation method and application thereof. The chitosan-based hydrogel is prepared with chitosan or a derivative thereof as a raw material bygrafting an ortho-phenyl dihydroxyl group compound onto the main chain of the raw material and then performing cross-linking via a cross-linking agent. The preparation method for the hydrogel comprises the following steps: firstly grafting an ortho-phenyl dihydroxy group onto chitosan or the derivative thereof to allow a product to have wet adhesion; and then, performing a cross-linking reaction to form the hydrogel material with sufficient mechanical strength and viscoelasticity. The hydrogel material has wet adhesion, hemostasis and wound healing promotion functions, and has good applicationprospects in tissue engineering and skin repairing.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and specifically relates to a chitosan-based hydrogel capable of adhering tissues in a wet environment, hemostasis and promoting healing, a preparation method thereof and an application as a tissue adhesive. Background technique [0002] Tissue adhesives refer to biomedical materials that can adhere to the surface of local tissues to cause adhesion between tissue surfaces. They are mainly used for local adhesion and repair of organs or tissues, auxiliary hemostasis of traditional sutures, and repair of teeth. , the combination and positioning of bones or joints and other technical fields. Its use can replace the traditional complex and time-consuming surgical suture or staple, which can quickly seal the wound, avoid wound infection, effectively reduce the effect of paralysis, and greatly improve the patient's medical comfort. In recent years, it has become more and more popular among do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00C08J3/075C08L5/08
CPCA61L24/001A61L24/0031A61L24/0042A61L24/08A61L2400/04A61L2400/14C08J3/075C08J5/08C08L5/08
Inventor 彭燕飞彭晓婷韩宝芹刘万顺
Owner OCEAN UNIV OF CHINA
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