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Early pancreatic cancer marker and detection method thereof

A detection method and marker technology, applied in biochemical equipment and methods, microbiological determination/testing, DNA/RNA fragments, etc., can solve the problems of low expression level, inability to truly reflect the actual condition of patients, missed diagnosis and misdiagnosis, etc.

Pending Publication Date: 2019-03-05
安科默(北京)生物技术有限公司
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AI Technical Summary

Problems solved by technology

Chinese patent application CN101942502A discloses 36 markers that can be used for the detection of pancreatic cancer, but the expression levels of the above markers in the plasma of pancreatic cancer patients are low, and the average clinical detection sensitivity is 80%, which cannot distinguish pancreatic cancer, pancreatitis and normal tissue changes
At the same time, due to the lack of comprehensive consideration of individual clinical characteristics, the test results of products containing this marker may not truly reflect the actual condition of the patient, resulting in missed diagnosis and misdiagnosis
In addition, the reason for the unstable test results of the existing technology is that the clinical sampling and sample preparation process will lead to a decrease in the quality of the analyte, interfere with the PCR reaction, and affect the final result; the preparation conditions for multiplex quantitative PCR are very critical, and incorrect probes and primers Both pairings result in loss of sensitivity

Method used

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  • Early pancreatic cancer marker and detection method thereof
  • Early pancreatic cancer marker and detection method thereof
  • Early pancreatic cancer marker and detection method thereof

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Experimental program
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Effect test

Embodiment 1

[0060] According to the American Joint Committee on Cancer (AJCC) tumor / node / metastasis (TNM) classification and gene expression classification, clinical samples were selected for experiments as shown in the table below. All retrospective clinical samples were obtained from the Creighton University Medical Center Hospital, the University of Nebraska Medical Research Center Affiliated Hospital, and the NIH Cancer Institute sample bank. Samples were selected according to the following criteria: 96 tissues from pancreatic cancer patients (45% of which were early stage), 56 tissues from patients with pancreatitis and 56 adjacent tissues from pancreatic cancer patients (as normal tissue control), of which Asians accounted for 11.2%; There were no significant statistical differences in age, gender, and drinking history, but there were significant statistical differences in smoking history and smoking and drinking history; all samples used to verify differentially expressed miRNAs wer...

Embodiment 2

[0064] 1. Prospective experiment

[0065] (1) Small clinical experiment (100 people): According to the database of Creighton University Proteomics and Metabolomics Research Center, 100 people were called to participate in the small clinical experiment (standards are as follows, Table 1):

[0066] Table 1: Characteristics and sources of pancreatic cancer clinical samples (prospective experiment) (Creighton University, UNMC Joint Pancreatic Cancer Research Center Cancer Sample Bank and Susanzuman Clinical Sample Bank, 2012-2014)

[0067]

[0068]

[0069]

[0070]

[0071]

[0072]

[0073]

[0074] The clinical design is as follows:

[0075] a, double-blind mixed prospective research method; b, pathological auxiliary verification; c, determination of patient characteristics; d, proportion of participants (N=100):

[0076] White population: 65%

[0077] Black people: 20%

[0078] Hispanic population: 12%

[0079] People of Asian descent: 3%

[0080] Mai...

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Abstract

The invention belongs to the technical field of biology and relates to an early pancreatic cancer marker and a detection method thereof. A biological micromolecule which can be used for early pancreatic cancer molecular diagnosis and treatment is developed on the basis of expression intensity differences of various micro ribonucleic acids in early pancreatic cancer cells. The early pancreatic cancer marker has advantages of wide detection pedigree, high sensitivity, high specificity, low detection cost, convenience in material acquisition, easiness in sample storage and the like. The method can be widely applied to pancreatic cancer early screening and prognosis and the like, low specificity and low sensitivity due to hard-to-overcome individual differences caused by intrinsic instabilityof single markers or biomarkers clinically and extensively applied at present are improved, the early pancreatic cancer clinical detection rate is remarkably increased, the pancreatic cancer misdiagnosis rate and missed diagnosis rate are decreased, and the method is an effective means of early pancreatic cancer detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for early screening and precise drug detection of microRNA in human body fluids in early pancreatic cancer. Background technique [0002] Pancreatic cancer is a lethal malignant disease with insidious onset and difficult early diagnosis. At present, there is no simple and practical detection method. Most of the clinically diagnosed patients are in the advanced stage, and the surgical resection rate is 10% to 20%. About 75% of the patients die within 1 year after diagnosis, and the 5-year survival rate is less than 5%. However, the 5-year survival rate can be increased to 19% to 41% for small pancreatic cancers limited to the pancreas and with a diameter of ≤2.0 cm after radical surgery. Obviously, early detection and early diagnosis are the key to effective treatment of pancreatic cancer and improvement of prognosis. [0003] Early pancreatic cancer usually refers to pancrea...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/106C12Q2600/112C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 肖桂山
Owner 安科默(北京)生物技术有限公司
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