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Preparation method and application of camphor essential oil-based bacterial quorum sensing inhibitor

A quorum sensing, camphor essential oil technology, applied in the directions of botanical equipment and methods, applications, chemicals used for biological control, etc., can solve the problems of unclear antibacterial activity of camphor leaf essential oil quorum sensing inhibitory activity, and achieves the preparation of Simple and reliable method, easy to obtain results

Inactive Publication Date: 2019-03-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report that this compound has the activity of inhibiting the QS activity of Violet Bacillus. It is still unclear whether the antibacterial activity of essential oil of camphor leaves is related to the quorum sensing inhibitory activity of essential oil of camphor leaves.

Method used

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  • Preparation method and application of camphor essential oil-based bacterial quorum sensing inhibitor
  • Preparation method and application of camphor essential oil-based bacterial quorum sensing inhibitor
  • Preparation method and application of camphor essential oil-based bacterial quorum sensing inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening of Bacterial Quorum Sensing Inhibitors

[0023] Extract polyphenols, polysaccharides, essential oils, alkaloids and other substances from the leaves, roots, and branches of camphor plants, and use the bacterial quorum sensing inhibitory activity as a tracking method to screen the effective parts and active ingredients with bacterial quorum sensing inhibitory activity. Cinnamomum camphora essential oil with obvious bacterial quorum sensing inhibitory activity was isolated from Cinnamomum camphora leaves.

Embodiment 2

[0024] Example Two: Extraction and Preparation of Camphor Essential Oil with Bacterial Quorum Sensing Inhibitory Activity

[0025] The processing method of camphor leaves: wash and dry the picked camphor leaves, and put them in a -20°C refrigerator for later use.

[0026] Weigh 50g of processed camphor leaves and place them in a round bottom flask, add distilled water according to the mass ratio of solid to liquid at 1:10, distill and extract in the essential oil extractor for 2 hours, collect the essential oil with a 2mL centrifuge tube after the extraction is completed, add 1 g of anhydrous sodium sulfate to remove excess water to obtain camphor essential oil with bacterial quorum sensing inhibitory activity.

Embodiment 3

[0027] Embodiment 3: Quorum sensing inhibition activity test of camphor essential oil

[0028] 1. Experimental samples and experimental methods

[0029] Pre-prepared wild-type violaceum Chromobacterium violaceum ATCC31532 was cultivated in liquid LB medium for 12 h at 30 °C and 150 rpm to obtain a bacterial suspension ( OD 600 , 0.9) Standby. Heat the solid LB medium (containing 2% agar), cool it to about 50 °C, add the bacterial suspension at 1% (V / V), shake well and quickly pour it into a petri dish, 20mL / dish. The filter paper method was used to study the inhibitory activity of bacterial quorum sensing. The specific method was as follows: a filter paper with a diameter of 6 mm was placed in the above-mentioned prepared medium, and 15 µL of camphor essential oil was injected into the filter paper, and the culture dish was sealed with a sealing film and placed in the culture medium. Incubate at 30°C for 24 h and observe. In order to verify whether the bacteria in the...

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Abstract

The invention provides a preparation method and application of a camphor essential oil-based bacterial quorum sensing inhibitor. The preparation method comprises the following steps: preparing essential oil and preparing the bacterial quorum sensing inhibitor. By adopting the preparation method, the camphor essential oil is obtained from camphor leaves, and for the first time, experiments confirmthat the essential oil prepared with the preparation method provided by the invention has the activity of inhibiting a quorum sensing system of wild purple bacillus, so that the purple bacillus is notpathogenic, and the essential oil does not cause the survival pressure on the purple bacillus, so that the drug resistance is generated; the essential oil prepared with the preparation method provided by the invention can inhibit the activity of escherichia coli, the purple bacillus, staphylococcus aureus and pseudomonas aeruginosa, and is a novel antibacterial substance based on bacterial quorumsensing inhibition. The method has the advantages and characteristics that the raw material is easy to obtain, the preparation method of the novel bacterial quorum sensing inhibitor is simple and reliable, the bacteriostatic agent does not generate drug resistance to bacteria and has no toxic effects on the bacteria and the like.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a preparation method and application of a bacterial quorum sensing inhibitor of camphor essential oil. Background technique [0002] In recent years, antibiotics and antibacterial drugs have been widely used in the prevention and treatment of bacterial diseases. Traditional antibacterial drugs inhibit and kill bacteria by interfering with the biochemical metabolic process of pathogenic bacteria, affecting their structure and function. Bacteria mutate rapidly under the huge pressure of survival, leading to the rapid increase of drug-resistant bacteria or superbugs, which brings many difficulties to the prevention and treatment of bacterial diseases. direction. Bacterial quorum sensing (QS) is a regulatory mechanism for bacterial quorum communication. It refers to bacteria producing and secreting specific signal molecules, and sensing changes in the numb...

Claims

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Application Information

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IPC IPC(8): A01N65/24A01P1/00
CPCA01N65/00A01N65/24
Inventor 李永裕王文婷吴少华张燎原吴英详张延辉刘靖媛葛一菲
Owner FUJIAN AGRI & FORESTRY UNIV
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