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Extraction method of cistanche chelating peptide

An extraction method, the technology of Cistanche deserticola, applied in the field of extraction of chelated peptides of Cistanche deserticola, can solve the problems of low yield, complicated extraction process, low purity and nutritional value of small molecule active peptides, and achieve the effect of improving flavor

Active Publication Date: 2019-03-15
HEBEI ZIFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the existing methods for extracting polypeptides from Cistanche deserticola, there are problems such as complex extraction process, low yield, purity of small molecule active peptides and low nutritional value, the present invention provides a method for extracting chelated peptides from Cistanche deserticola

Method used

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  • Extraction method of cistanche chelating peptide
  • Extraction method of cistanche chelating peptide
  • Extraction method of cistanche chelating peptide

Examples

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Embodiment 1

[0028] A method for extracting chelated peptides from Cistanche deserticola, comprising the following processing steps:

[0029] a. After drying 1000g of cistanche, crush it, add 7000g of purified water, and decoct for 4 hours at 90°C;

[0030] b. Cool down to 55°C, add 10g of 20U / mg amylase, 5g of 20U / mg glucoamylase and 10g of 20U / mg xylanase, and stir for 20 minutes; wherein the xylanase is fermented by Trichoderma Extracted, degradable cell wall exists in hemicellulose polysaccharides.

[0031] c. Add 10g of 20U / mg bromelain and 5g of 20U / mg cathepsin B in the mass of raw materials, keep the temperature at 55°C, and stir for 3 hours; wherein, bromelain is extracted from pineapple fruit and stem by pressing, and pineapple Protease can hydrolyze proteins or macromolecular polypeptides into low molecular weight small peptides; cathepsin B is extracted from carp, and has the functions of both endonuclease and telopeptidase. It has strong hydrolysis ability and can effectively...

Embodiment 2

[0037] A method for extracting chelated peptides from Cistanche deserticola, comprising the following processing steps:

[0038] a. Dry 1000g of Cistanche cistanche, crush it, add 8000g of purified water, and decoct for 4.5 hours at 95°C;

[0039] b. Cool down to 56°C, add 12g of 20U / mg amylase, 6g of 20U / mg glucoamylase and 12g of 20U / mg xylanase, and stir for 25 minutes; wherein the xylanase is fermented by Trichoderma Extracted, degradable cell wall exists in hemicellulose polysaccharides.

[0040] c. Add 13g of 20U / mg bromelain and 7g of 20U / mg cathepsin B, keep the temperature at 56°C, and stir for 3h; wherein, bromelain is extracted from pineapple fruit and stem through pressing, and bromelain can make Proteins or macromolecular polypeptides are hydrolyzed into low-molecular-weight small peptides; cathepsin B is extracted from carp, and it has the functions of endonuclease and telopeptidase. Peptides are thoroughly hydrolyzed to improve the flavor of peptide products. ...

Embodiment 3

[0046] A method for extracting chelated peptides from Cistanche deserticola, comprising the following processing steps:

[0047] a. After drying 1000g of cistanche, pulverize, add 8000g of purified water, and decoct for 5 hours at 95°C;

[0048] b. Cool down to 58°C, add 15g of 20U / mg amylase, 8g of 20U / mg glucoamylase and 15g of 20U / mg xylanase, and stir for 30 minutes; wherein the xylanase is fermented by Trichoderma Extracted, degradable cell wall exists in hemicellulose polysaccharides.

[0049] c. Add 15g of 20U / mg bromelain and 8g of 20U / mg cathepsin B, keep the temperature at 58°C, and stir for 4 hours; wherein, bromelain is extracted from pineapple fruit and stem through pressing, and bromelain can make Proteins or macromolecular polypeptides are hydrolyzed into low-molecular-weight small peptides; cathepsin B is extracted from carp, which has the functions of endonuclease and telopeptidase, and has strong hydrolysis ability, which can effectively recognize bitter pep...

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Abstract

The invention relates to the technical field of deep processing of cistanche and particularly discloses an extraction method of cistanche chelating peptide. The extraction method of the cistanche chelating peptide comprises the following process steps: a, drying and pulverizing cistanche, adding purified water and performing decoction; b, adding amylase, saccharifying enzyme and xylanase; c, adding bromelain and cathepsin B; d, heating and performing enzyme deactivation; e, performing centrifugal purification; f, adding soybean peptide and stirring for 30 to 45 minutes; and g, concentrating and drying reaction liquid. By methods for degrading protein through targeted enzymolysis and directed enzyme digestion as well as a nanofiltration technology, the extraction method of the cistanche chelating peptide has the advantages of short production cycle, low cost and no environmental pollution; furthermore, the obtained product has high safety, high purity and high bioactivity, is low in molecular weight and can be widely applied in the fields of food, medicines and the like.

Description

technical field [0001] The invention relates to the technical field of deep processing of Cistanche deserticola, in particular to a method for extracting chelated peptides of Cistanche deserticola. Background technique [0002] Cistanche deserticola is mainly produced in Xinjiang and Alxa League of Inner Mongolia, and is also distributed in Gansu and Ningxia. It likes to grow on soft sandy land with mild salinization, and generally grows in sandy land or semi-fixed sand dunes, dry old river beds, lake basin lowlands, etc. The growing conditions are poor. The climate in the suitable growing area is dry, with little rainfall, large evaporation, long sunshine hours, and large temperature difference between day and night. The soil is mainly gray brown desert soil and brown desert soil. The host Haloxylon Haloxylon is a strong xerophyte. Cistanche deserticola is mostly parasitic on its lateral roots at a depth of 30-100 cm. It is born in the desert at an altitude of 225-1150 m....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张晓东杨广杨子丰
Owner HEBEI ZIFENG BIOTECH