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A HPLC method for the simultaneous determination of eight active components in Codonopsis pilosula

A technology for active ingredients and ginseng medicinal materials, applied in the field of quality control of traditional Chinese medicinal materials and their preparations, can solve problems such as insufficient description of the quality of Codonopsis pilosula medicinal materials, and achieve the effects of good linear relationship, simple operation and rapid separation

Active Publication Date: 2021-06-18
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

In the Pharmacopoeia, tangsheynoside is regarded as the symbol compound of Codonopsis pilosula, but some studies have found that tangsheynoside exists not only in genuine Codonopsis pilosula, but also in substitutes, and even tangsheynoside has been detected in other plants of the same family. Glycoside content is not enough to explain the quality of Codonopsis pilosula

Method used

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  • A HPLC method for the simultaneous determination of eight active components in Codonopsis pilosula
  • A HPLC method for the simultaneous determination of eight active components in Codonopsis pilosula
  • A HPLC method for the simultaneous determination of eight active components in Codonopsis pilosula

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Embodiment

[0019] Standard solution preparation: Accurately weigh 10mg, 9.9mg, 10mg, 10.3 mg, 40mg, 10.7mg, 9.5mg, 10.1mg, add an appropriate amount of methanol to dissolve, respectively dilute to the mark in a 10mL volumetric flask, and shake well to obtain tangshenoside, nicotinic acid, adenosine, syringin, succinic acid, a The concentrations of fulmic acid, 5-hydroxymethylfurfural and atractylodele III are 1mg / mL, 0.99mg / mL, 1mg / mL, 0.103mg / mL, 4mg / mL, 0.107mg / mL, 0.95mg / mL, 0.101 mg / mL. Store them separately as standard stock solution for future use.

[0020] Preparation of sample solution: Accurately weigh 1 g of dried Codonopsis pilosula, place in a 150 mL Erlenmeyer flask, add 25 mL of methanol, ultrasonicate for 15 min, take the supernatant and filter it with a 0.45 μm filter membrane, and store it for later use.

[0021] Establishment of standard curves for the determination of active components in eight Codonopsis medicinal materials: the chromatographic conditions are C18 ch...

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Abstract

The invention discloses an HPLC method for simultaneous determination of eight active components in Codonopsis pilosula. , Atractylodes Lactone III and 5-Hydroxymethylfurfural, its assay steps are as follows: (1) preparation of test solution; (2) chromatographic condition: C18 chromatographic column, mobile phase consists of methanol-0.01% phosphoric acid aqueous solution, gradient Elution program: 0‑10min, 10% methanol; 10‑25min, 10%‑65% methanol; 25‑40min, 65%‑80% methanol. Wavelength: 0‑5min, 260nm; 5‑6.5min, 210nm; 6.5‑36.5min, 260nm; 36.5‑38min, 220nm; 38‑40min, 260nm. Flow rate 1 mL / min. Column temperature: 30°C. The injection volume was 10 μL. (3) Establishment of standard curve for the determination of the content of eight active ingredients; (4) Determination of the content of active ingredients in the sample. The method is easy to operate, accurate, fast and efficient, can simultaneously measure the contents of eight active components in Codonopsis pilosula medicinal materials, and has great practical value.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicinal materials and their preparations, and relates to the simultaneous determination of tangshenoside, nicotinic acid, adenosine, syringin, succinic acid, ferulic acid, atractylide III and 5-hydroxymethylfurfural in Codonopsis pilosula Eight active ingredients. Background technique [0002] Codonopsis Radix (Codonopsis Radix) is a commonly used clinical traditional Chinese medicinal material. The Codonopsis Radix recorded in the 2015 edition of the Pharmacopoeia of the People's Republic of China mainly includes three types of Codonopsis Radix, Codonopsis Radix, or Sichuan Codonopsis Radix. It has the effects of invigorating the spleen and lungs, nourishing blood and promoting body fluid, and has important clinical applications. [0003] Codonopsis pilosula contains a variety of active ingredients, mainly including polysaccharides, polyacetylenes, alkaloids and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赵华何金莲刘珊郑雯蓝蒋晓丹
Owner CHONGQING MEDICAL UNIVERSITY
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