Method of establishing chronic ocular hypertension animal model

Abstract

The invention relates to a method of establishing a chronic ocular hypertension animal model. The method includes: dilating pupils of eyes of an animal model, and after a predetermined time, subjecting the ocular surface of the animal model to local anesthesia; after the pupils of the eyes of the animal model are dilated, placing magnet rings above the eyeballs of the animal model by centering onthe pupils; dispersing silica magnetic microspheres in normal saline to obtain silica magnetic microsphere suspension for use; extracting anterior chamber fluid from the eyes of the animal model, andinjecting the silica magnetic microsphere suspension, sodium hyaluronate gel and air sequentially into the anterior chambers of the eyes of the animal model, and after the silica magnetic microspheresinjected are dispersed magnetically and evenly to the anterior chamber corners through the magnet rings, acquiring the chronic ocular hypertension animal model. The method provided herein helps evidently extend the ocular hypertension maintenance time and attain the stable and effective chromonic ocular hypertension animal model.

Description

technical field [0001] The invention belongs to the technical field of establishing animal experimental models, and in particular relates to a method for establishing an animal model of chronic high intraocular pressure. Background technique [0002] Glaucoma is a group of diseases characterized by optic nerve pitting atrophy and visual field defect, and its main risk factor is the increase of pathological intraocular pressure IOP (Intraocular Pressure). The main cause of glaucoma blindness is: the oppression and damage of high intraocular pressure on intraocular tissues, especially the ONH (Optic Nerve Head) in the posterior segment of the eye, resulting in optic atrophy and visual field loss. At present, the most important treatment for glaucoma is to reduce intraocular pressure through drugs or surgery to prevent or delay the progression of the disease. Therefore, in the field of glaucoma research, animal models of ocular hypertension play an important role in better und...

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Problems solved by technology

[0003] At present, the more commonly used animal models of ocular hypertension include: (1) posterior chamber injection of α-chymotrypsin or carbomer ocular hypertension model: the disadvantage of this model is that it can cause more obvious inflammatory reactions, and the intraocular pressure rises suddenly , with a short maintenance time and low success rate, it is easy to cause many complications (such as lens opacity, dislocation, anterior chamber exudation, corneal edema and lens atrophy, etc.); (2) Anterior chamber injection of sodium hyaluronate high intraocular pressure model: The disadvantage of this model is that repeated injections are required to maintain high intraocular pressure, and the increase in intraocular pressure is not obvious. Usually, simple injection of sodium hyaluronate into the anterior chamber can only maintain high intraocular pressure for 5 days. Repeated injections of sodium hyaluronate are required every 5 days; (3) Laser photocoagulation of the sclera or laser photocoagulation of trabecular meshwork high intraocular pressure model: the disadvantages of this model are difficult operation, high cost, and maintenance of high intraocular pressure If the time is short, repeated laser photocoagulation is required to maintain high intraocular pressure for a long time, but it is easy to cause intraocular congestion and inflammatory reactions; (4) Anterior chamber injection of polystyrene microspheres to establish a chronic high intraocular pressure model: the The disadvantage of the model is that the distribution of polystyrene microspheres in the angle of the chamber is uneven, resulting in an unstable increase in intraocular pressure, and repeated injections of microspheres are required to maintain high intraocular pressure for a long time

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