Method for extracting multifunctional bioactive peptide from loach

Abstract

The invention belongs to the technical field of the food processing, and particularly relates to a method for extracting multifunctional bioactive peptide from loach. The method comprises six steps ofsmashing the loach, operating in a constant temperature, performing enzymolysis, performing enzyme deactivation, centrifuging and drying, so extraction to the multifunctional bioactive peptide is completed. Through adding complex enzyme and controlling reaction conditions, an extraction ratio of a product and product quality are improved. The extracted multifunctional bioactive peptide has the efficacy of bacteriostasis, immunity enhancement and anti-fatigue.

Description

technical field [0001] The invention belongs to the technical field of food processing, in particular to a method for extracting multifunctional active peptides from loach. Background technique [0002] Loach is rich in protein, amino acids, minerals, unsaturated fatty acids and various active substances, etc. Its meat is tender and delicious, and it is a common food for fish. Efficacy, it can be used as an auxiliary food for removing dryness, preventing impotence, yellowing of epidemics, night sweats in children, hemorrhoids, scabies and other diseases. Loach also has a significant effect on improving liver function in patients with chronic hepatitis. In addition, loach also has a low fat content. Therefore, it is beneficial for patients with cardiovascular disease, anemia, and hepatitis to eat more; then, my country's current cultured loach is mainly used for live sales, cooking and eating for families or restaurants, and there are fewer deep-processing developments. low val...

AI Technical Summary

Problems solved by technology

[0003] There are many reports about the extraction of loach in the prior art, such as the preparation of loach antimicrobial peptide and the research on its antibacterial effect in the literature "The preparation of loach antimicrobial peptide and the study of its antibacterial effect" adopt the Tris-HCL method to extract the loach antimicrobial peptide with bactericidal effect, and its antibacterial effect on hydrophile Monascus, Escherichia coli and Staphylococcus aureus have a certain bactericidal effect. For example, in "Optimization of Loach Protein Enzymatic Hydrolysis Process Conditions", by optimizing the enzymatic hydrolysis conditions, it is obtained that [E] / [S]4.9%, substrate The mass fraction is 30%, the pH is 6.5, the enzymolysis temperature is 54.4°C, and the enzymolysis time is 5.9h, the ACE inhibition rate of the prepared loach protein hydrolyzate reaches 88.92%. It can be seen that loach contains a variety of different effects Active ingredients, and these ingredients are obtained through different extraction processes to obtain peptides with different functions. However, in the prior art, the peptides obtained by most loach extraction processes have relatively single efficacy and unsatisfactory extraction effects, which limits the loach industry. development, for this reason, the present invention provides a kind of extraction method of loach multifunctional peptide