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Composite functional microbial preparation, and preparation method and application thereof

A technology of composite microbial bacterial agent and microbial preparation, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of low total heavy metal enrichment, long time for soil remediation, and remediation efficiency. Low-level problems, to achieve the effect of reducing toxicity, increasing organic matter content, and increasing biomass

Inactive Publication Date: 2019-03-26
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the impact of heavy metal stress, a large number of energy plants often grow slowly, have small biomass, and the total accumulation of heavy metals is low, so the time for soil restoration is relatively long, and the restoration efficiency is also low.
[0003] At present, there is no special bacterial agent that can not only reduce the concentration of heavy metals in the soil environment, but also promote plant growth and effectively improve the efficiency of phytoremediation of heavy metal-contaminated soil. Therefore, it is necessary to invent a method that is specific to the growth characteristics of plants (especially energy plants). , a special bacterial agent specially used to promote plant remediation of heavy metal-contaminated soil, to effectively reduce the toxicity of lead and zinc in soil, improve the growth of plants, and improve the remediation efficiency of contaminated soil

Method used

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  • Composite functional microbial preparation, and preparation method and application thereof
  • Composite functional microbial preparation, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A composite functional microbial preparation, in parts by mass, including 7 parts by mass of basic nutrient solution, 92 parts by mass of attachment matrix, and 1 part by mass of composite microbial bacterial agent, wherein the composite microbial bacterial agent includes Verticillium entomobilis bacterial agent , Aspergillus oryzae, Bacillus coliformis and Bacillus subtilis. The mass percent composition of Verticillium entomogenes inoculum in the composite microbial inoculum is 43%, the mass percent composition of Aspergillus oryzae inoculum is 29%, and the mass percent composition of colloid bacillus inoculum is 14%. The mass percent content of the Bacillus subtilis bacterial agent is 14%.

[0038] In this embodiment, the basic nutrient solution includes calcium superphosphate, potassium sulfate, sodium hydroxide, disodium hydrogen phosphate and sodium dihydrogen phosphate, calcium superphosphate, potassium sulfate, sodium hydroxide, disodium hydrogen phosphate and so...

Embodiment 2

[0059] The application of a kind of composite functional microbial preparation in repairing heavy metal polluted soil is specifically to carry out plant cultivation in soil polluted with different concentrations of zinc, comprising the following steps:

[0060] Experimental group: According to the mass ratio of 1:50, the composite functional microbial preparation prepared in Example 1 was mixed evenly with zinc-contaminated soil, and potted. A total of 6 pots of parallel samples were set up, and 3 peanuts were planted in each parallel sample.

[0061] Control group: without adding any microbial agent, the quality of the compound functional microbial preparation is supplemented with the same amount of zinc-contaminated soil, and the other conditions are the same as the experimental group. A total of 6 pots of parallel samples were set up, and 3 peanuts were planted in each parallel sample.

[0062] After 8 weeks of planting, the changes in the experimental survival rate, plant...

Embodiment 3

[0067] An application of a compound functional microbial preparation in repairing heavy metal-contaminated soil, specifically for plant cultivation in lead-contaminated soil with different concentrations, comprising the following steps:

[0068] Experimental group: According to the mass ratio of 1:50, mix the composite functional microbial preparation prepared in Example 1 and the lead-contaminated soil evenly, and put them into pots. A total of 6 pots of parallel samples were set up, and 3 sunflowers were planted in each parallel sample.

[0069] Control group: No bacterial agent was added, and the quality of the composite functional microbial preparation was supplemented with the same amount of lead-contaminated soil, and the other conditions were the same as the experimental group. A total of 6 pots of parallel samples were set up, and 3 sunflowers were planted in each parallel sample.

[0070] After 4 weeks of planting, the changes in the experimental survival rate, plant...

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Abstract

The invention discloses a composite functional microbial preparation, and a preparation method and application thereof. The composite functional microbial preparation is prepared from the following components in parts by mass: 85-93 parts of attachment matrixes, 2-8 parts of basic nutrient solutions and 1-3 parts of composite microbial agents, wherein the composite microbial agents contain entomogenous verticillium agents, aspergillus oryzae agents, bacillus mucilaginosus agents and bacillus subtilis agents. The preparation method includes the steps of mixing the basic nutrient solutions withthe attachment matrixes, aging, and adding the composite microbial agents for fermentation to obtain the preparation. The preparation can effectively alleviate the toxicity of heavy metals in the plant rhizosphere soil, and can improve the soil structure and the plant root environment, enhance soil nutrition and promote plant growth; the preparation has the advantages of simple process, low production cost and the like, and can adapt to large-scale industrial production; the preparation provided by the invention can be used for promoting energy plant restoration of heavy-metal contaminated soil, thereby having good application value and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and relates to a multifunctional microbial preparation and its preparation method and application. Background technique [0002] The use of plant (such as energy plant) remediation technology to treat heavy metal-contaminated soil can organically combine soil remediation technology with bioenergy production, taking into account both ecological and economic benefits, and has broad application prospects. However, due to the impact of heavy metal stress, a large number of energy plants often grow slowly, have small biomass, and the total amount of heavy metal enrichment is low, so the time for soil restoration is relatively long, and the restoration efficiency is also low. [0003] At present, there is no special bacterial agent that can not only reduce the concentration of heavy metals in the soil environment, but also promote plant growth and effectively improve the efficiency of ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C12N1/14C12N1/20C12N1/36C12R1/645C12R1/69C12R1/07C12R1/125C05G3/80
CPCC05B1/02C05G3/00C12N1/14C12N1/20C12N1/36C05G3/80C05B7/00C05D1/02C05D9/00C05F5/002C05F11/08
Inventor 冯冲凌李雪陶雅妮吉巧玲
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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