Monascus purpureus induced strain and water-soluble functional monascus as well as preparation method and application of water-soluble functional monascus

A technology of Monascus purple and mutagenic strains, applied in the biological field, can solve the problems of solid-state fermentation products such as severe bitterness, difficult product, and difficult secretion of intracellular and extracellular metabolites, and achieve easy large-scale production, increased application range, and good water solubility Effect

Active Publication Date: 2019-03-26
GUANGDONG TIANYI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitation of solid-state fermentation conditions, it is difficult to regulate the secretion of extracellular and extracellular metabolites in the solid-state fermentation of the triangular flask. The production of red yeast rice with high ring-opening function has not been reported, and the solid-state fermentation product has a severe bitter taste and is difficult to

Method used

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  • Monascus purpureus induced strain and water-soluble functional monascus as well as preparation method and application of water-soluble functional monascus
  • Monascus purpureus induced strain and water-soluble functional monascus as well as preparation method and application of water-soluble functional monascus
  • Monascus purpureus induced strain and water-soluble functional monascus as well as preparation method and application of water-soluble functional monascus

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0064] Example 1

[0065] (1) Preparation of seed liquid

[0066] Preparation of first-level seed liquid:

[0067] A mutagenic strain of Monascus purpureus Went was used as the production seed. Inoculate the seed culture medium (glucose 5%, peptone 2.0%, corn steep liquor 0.5%, NaNO) with shake flask seed solution at 5% inoculation amount (V / V) 3 0.1 %, MgSO4•7H 2 O 0.05%, K 2 HPO 4 • 3H 2 O 0.05%, the components are calculated by weight-volume ratio, the unit is kg / m 3 ; PH value (natural) for culture and propagation, 30°C, 150 rpm, ventilation ratio 1:1.5, tank pressure 0.04Mpa, culture for 36 hours, so that the bacteria are in the logarithmic growth phase.

[0068] Preparation of secondary seed liquid:

[0069] The first-level seed solution in the logarithmic growth phase is inoculated into a sterilized and cooled seed medium (glucose 6%, peptone 2.5%, corn steep liquor 0.8%, NaNO) at a 6% inoculum amount (V / V) 3 0.12 %, MgSO 4 • 7H 2 O 0.05%, K 2 HPO 4 • 3H 2 O0.05%, the componen...

Example Embodiment

[0073] Example 2

[0074] (1) Preparation of seed liquid

[0075] Preparation of first-level seed liquid:

[0076] A mutagenic strain of Monascus purpureus Went was used as the production seed. Inoculate the seed medium (glucose 6%, peptone 2.5%, corn steep liquor 0.8%, NaNO 3 0.15 %, MgSO4•7H 2 O 0.08%, K 2 HPO 4 • 3H 2 O 0.08%, the components are calculated by weight to volume ratio, the unit is kg / m 3 ;PH value (natural) culture and proliferation, 31℃, 180 rpm, ventilation ratio 1:1.6, tank pressure 0.04Mpa, culture for 32h, make the bacteria in the logarithmic growth phase.

[0077] Preparation of secondary seed liquid:

[0078] The first-level seed solution in the logarithmic growth phase was inoculated into sterilized and cooled seed medium (glucose 6.5 %, peptone 2.8%, corn steep liquor 1.0%, NaNO) at 8% inoculum amount (V / V) 3 0.2 %, MgSO 4 • 7H 2 O 0.08%, K 2 HPO 4 • 3H 2 O 0.08%, the components are calculated by weight to volume ratio, the unit is kg / m 3 ;PH value (natural) ...

Example Embodiment

[0082] Example 3

[0083] (1) Preparation of seed liquid

[0084] Preparation of first-level seed liquid:

[0085] A mutagenic strain of Monascus purpureus Went was used as the production seed. Inoculate the seed medium (glucose 8%, peptone 2.8%, corn steep liquor 1.0%, NaNO) with 8% inoculum amount (V / V) of shake flask seed solution 3 0.2 %, MgSO4•7H 2 O 0.1%, K 2 HPO 4 • 3H 2 O 0.1%, the components are calculated by weight-volume ratio, the unit is kg / m 3 ;PH value (natural) culture and proliferation, 32 ℃, 200 rpm, ventilation ratio of 1:1.8, tank pressure of 0.05Mpa, culture for 24h, so that the bacteria in the logarithmic growth phase.

[0086] Preparation of secondary seed liquid:

[0087] The first-level seed solution in the logarithmic growth phase was inoculated into sterile and cooled seed culture medium (glucose 8.5%, peptone 3.0%, corn steep liquor 1.2%, NaNO) at 10% inoculum amount (V / V) 3 0.25 %, MgSO 4 • 7H 2 O 0.12%, K 2 HPO 4 • 3H 2 O 0.12%, the components are calcul...

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Abstract

The invention discloses a monascus purpureus induced strain and water-soluble functional monascus as well as a preparation method and application of water-soluble functional monascus. The monascus purpureus induced strain is preserved at China Center for Type Culture Collection on September 3, 2018, and the preservation number is CCTCC NO: M2018586. The monascus purpureus induced strain can be used for producing high-open-loop functional monascus and hardly produces citrinin. In addition, the monascus purpureus induced strain is arranged in a special fermentation culture medium to be further fermented in a liquid state to efficiently synthesize monacolin K with a high-open-loop structure, then the nonacolin K is processed by virtue of an after-treatment process to obtain the water-solublefunctional monascus, and the water-soluble functional monascus adopting a high-open-loop acidic structure as a main body has the characteristics of good water solubility, excellent flavor, no citrininor extremely low content of the citrinin. Moreover, a fermentation raw material is a non-glycerinum raw material, so that the potential safety hazard of glycerinum residue can be avoided. The preparation method provided by the invention has the advantages of being simple in process, easy in mass production, pollution-free and environmentally friendly; and the prepared product meets the safety quality requirement of food, healthcare products and drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a mutagenic strain of Monascus purple, a water-soluble functional red yeast rice, a preparation method and application thereof. Background technique [0002] Red yeast rice has the effects of invigorating the spleen and stomach, promoting blood circulation, removing blood stasis and relieving pain. It has a long history of edible and medicinal use in my country. In 1979, Endo et al. found that the Monacolin series of substances contained in the red yeast rice of a special Monascus can inhibit the HMG-CoA reductase (HMG-CoA reductase) in the biosynthesis of cholesterol in the human body. The role of this kind of red yeast rice is called lipid-lowering red yeast rice, also known as functional red yeast rice. [0003] With the gradual improvement of people's living standards and the increasingly obvious problem of aging population, the incidence of hyperlipidemia is increasing yea...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L33/00A61K36/06A61P3/06C12R1/645
CPCA23V2002/00A61K36/06A23L33/00A61P3/06C12N1/145C12R2001/645A23V2200/3262
Inventor 许世锦胡文林陈映妮聂增宇陈玲娟陈晓锋
Owner GUANGDONG TIANYI BIOTECH CO LTD
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