Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis

A technology of Bacillus licheniformis and microbial fermentation, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, to achieve the effects of facilitating digestion and absorption, short fermentation time, and high number of viable bacteria

Inactive Publication Date: 2019-03-26
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no Bacillus licheniformis with higher cellulase

Method used

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  • Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis
  • Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis
  • Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis

Examples

Experimental program
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Example Embodiment

[0058] Example 1: Isolation and screening of Bacillus licheniformis

[0059] Take 10g of Shanxi Fenjiu Daqu sample in 90mL sterile saline, place it on a shaker, shake at 30℃ and 200rpm for 30min, and then dilute it by 10 times to 10 -8 . Take 1mL10 -8 Add 20 mL of 80 ℃ Bacillus licheniformis solid medium in a molten state, mix and cool to room temperature, and place it in a 30 ℃ constant temperature incubator for 24 hours after solidification. After 24 hours, multiple colonies can be observed on the plate. The colonies are round colonies with irregular edges and wrinkles on the surface. They are positive by Gram staining. Observed under an optical microscope with a 100x oil lens, the bacteria are The morphology grows in rod-like, single, paired or chain-like arrangement; this feature is the same as that of Bacillus licheniformis.

[0060] Pick a single colony (concentration of 10 9 CFU / mL) was inoculated on the CMC selection medium plate, and incubated at 30°C for 24 hours. After...

Example Embodiment

[0062] Example 2: Identification of Bacillus licheniformis 50

[0063] 2.1 Identification of colony morphology

[0064] Observe the biological characteristics of the strains isolated in Example 1, and conduct further morphological identification (including observation of the morphology and edge of the colony, Gram staining, etc.). The above identification criteria refer to the "Berger Bacterial Identification Manual", scientific publication Society, 1984 and "Manual of Identification of Common Bacterial Systems", Science Press, 2001. After the isolated strain was cultured on the solid medium of Bacillus licheniformis at 30°C for 24 hours, the colony shape was round colony with irregular edges and wrinkles on the surface. It was positive by Gram staining; it was confirmed by the oil lens at 100 times of the optical microscope Under observation, the morphology of the bacteria grows rod-shaped, single, paired or chained; this feature is the same as that of Bacillus licheniformis.

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Example Embodiment

[0069] Example 3 Preparation of microbial starter

[0070] Obtain the culture broth of Bacillus licheniformis 50, Lactobacillus plantarum (isolated from commercially available fermented milk) and Candida utilis (isolated from commercially available fermented feed) through the following steps:

[0071] (1) Bacillus licheniformis: The Bacillus licheniformis 50 obtained in Example 1 was inoculated into the Bacillus licheniformis culture medium at an inoculum of 1 v / v%, placed on a constant temperature shaker, and cultured overnight at 30°C and 200 rpm.

[0072] (2) Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium at an inoculum of 1 v / v%, placed on a constant temperature shaker, and cultivated overnight at 30°C and 200 rpm.

[0073] (3) Candida utilis: The Candida utilis was inoculated into YPD medium at an inoculum of 1 v / v%, placed on a constant temperature shaker, and cultured overnight at 30°C and 200 rpm.

[0074] The GB4789.2-2006 method was used to det...

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Abstract

The invention relates to cellulase-producing bacillus licheniformis 50, a microbial fermentation preparation thereof and application of of the cellulase-producing bacillus licheniformis 50. The cellulase-producing bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.15012. The bacillus licheniformis 50 disclosed by the invention can produce cellulase with high yield and improve the degradation capacity on cellulose in corn processing by-products, and can be applied to feed fermentation with the cornprocessing by-products as raw materials to decrease the fiber content in fermented feed, so as to improve the quality of the feed and facilitate the efficient use of the feed by livestock and poultry.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular, the invention relates to a cellulase-producing Bacillus licheniformis, a microbial fermentation preparation containing the strain and its application in the fermentation of feed containing corn processing by-products. Background technique [0002] Corn is the main crop product in my country, with an annual output of about 200 million tons. Among them, for every ton of corn directly processed, 0.09 tons of corn steep liquor, 0.07 tons of germ meal, 0.056 tons of corn gluten meal and 0.13 tons of corn bran can be produced; at the same time, corn is used as raw material for fermentation to produce many fermentation products, such as fuel ethanol , citric acid, amino acids, etc.; can also produce a large number of by-products, such as DDGS, citric acid residues, etc. Corn processing products such as corn bran, DDGS, citric acid grains, etc. are usually directly added to feed for ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K10/12A23K10/37A23K10/30A23K10/38C12R1/10
CPCA23K10/12A23K10/30A23K10/37A23K10/38C12N1/16C12N1/20C12R2001/10C12N1/205A23V2400/517A23V2400/169Y02P60/87
Inventor 佟毅张子剑杨鑫郑晓卫陈博卢宗梅陈影安泰
Owner JILIN COFCO BIOCHEM
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