Method for combined strengthening of soil body with plant mucilage-microorganism

A plant mucus and joint reinforcement technology, applied in the field of soil reinforcement, achieves the effect of improving brittle deformation characteristics, low cost, and simple process

Inactive Publication Date: 2019-03-26
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, microorganism-induced calcium carbonate precipitation to solidify soil is an emerging reinforcement technology in recent years, but a major defect of this technology is that the calcium carbonate formed between particles has the characteristics of brittle deformation. In order to overcome this defect, it is necessary to Incorporation of gelatinous substances during microbial mineralization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for plant mucus-microorganism joint reinforcement soil, characterized in that: the method comprises the following steps:

[0026] 1) Inoculate Bacillus pasteurianus on the peptone-yeast powder-urea medium, the concentration of each component of the medium is: peptone 5g / L, yeast extract 3g / L, urea 20g / L, manganese sulfate 10g / L , and then shake culture at 30°C at an oscillation frequency of 160rpm for 24h, then centrifuge at 8000rpm for 8min, remove the supernatant, collect the strains with the culture medium to obtain the microbial liquid, and control the OD of the microbial liquid 600 is 1.0;

[0027] 2) Mixing and stirring the microbial liquid and the plant mucus evenly according to the volume ratio of 1:10, to obtain the bacterial liquid mucus mixture;

[0028] 3) Mix urea and calcium chloride according to the mass ratio of 1:1 to configure a nutrient solution, wherein the total concentration of urea and calcium chloride is 1.5mol / L;

[0029] 4) Spray or ...

Embodiment 2

[0031] A method for plant mucus-microorganism joint reinforcement soil, characterized in that: the method comprises the following steps:

[0032] 1) Inoculate Bacillus pasteurianus or other urease-producing bacteria on the peptone-yeast powder-urea medium, the concentration of each component of the medium is: peptone 5g / L, yeast extract 3g / L, urea 20g / L, Manganese sulfate 10g / L, then vibrated at 25°C at 180rpm for 28h, then centrifuged at 4000rpm for 15min, removed the supernatant, collected the strains with the culture medium to obtain the microbial liquid, and controlled the OD of the microbial liquid 600 is 1.2;

[0033] 2) Mixing and stirring the microbial liquid and the plant mucus evenly according to the volume ratio of 1:20 to obtain the bacterial liquid mucus mixture;

[0034] 3) Mix urea and calcium nitrate according to the mass ratio of 1.5:1 to configure a nutrient solution, wherein the total concentration of urea and calcium salt is 2mol / L;

[0035] 4) Spray or i...

Embodiment 3

[0037] A method for plant mucus-microorganism joint reinforcement soil, characterized in that: the method comprises the following steps:

[0038] 1) Inoculate Bacillus lentus on the peptone-yeast powder-urea medium, the concentration of each component of the medium is: peptone 10g / L, yeast extract 6g / L, urea 25g / L, manganese sulfate 10g / L, Then shake and culture at 170rpm at 35°C for 22 hours, then centrifuge at 6000rpm for 12min, remove the supernatant, collect the strains with the culture medium to obtain the microbial liquid, and control the OD of the microbial liquid 600 is 0.8;

[0039] 2) Mixing and stirring the microbial liquid and the plant mucus evenly according to the volume ratio of 1:15 to obtain the bacterial liquid mucus mixture;

[0040] 3) Mix urea and calcium lactate in a mass ratio of 2:1 to configure a nutrient solution, wherein the total concentration of urea and calcium lactate is 1mol / L;

[0041] 4) Spray or inject the mixture of bacterial fluid and muc...

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PUM

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Abstract

The invention discloses a method for combined strengthening of soil body with plant mucilage-microorganism. The method for combined strengthening of the soil body with plant mucilage-microorganism comprises the following steps that 1) Bacillus pasteurii or Bacillus lentus is inoculated on a culture media; and then shake culture is conducted, supernatant is removed centrifugally, and a microorganism bacteria solution is acquired; and 2) the microorganism bacteria solution and plant mucilage are mixed and stirred uniformly, and a mixture of the bacteria solution and mucilage is acquired; and then the mixture of the bacteria solution and mucilage and a nutrient solution are added into the soil body step by step so that strengthening can be conducted. According to the method for combined strengthening of the soil body with plant mucilage-microorganism, the polysaccharide gelatinization characteristic of plant mucilage and the characteristic that calcium carbonate is generated by mineralization of microorganism are utilized so that strengthening can be conducted on the soil body; and the method is characterized in that the effect is good, the cost is low, and in addition, no pollution is caused to the environment, and the ecological equilibrium is protected.

Description

[0001] Field [0002] The invention relates to a method for jointly reinforcing soil with plant mucus and microbes, and belongs to the technical field of soil reinforcement. Background technique [0003] Soil reinforcement has always been an urgent problem in geotechnical engineering. Many engineering accidents are caused by insufficient soil strength, such as subsidence of roadbed, instability of dams, slopes and sand dunes, erosion of river banks and liquefaction of foundation soil, etc., and the problem of soil penetration is also an important issue in engineering. question. [0004] The traditional soil reinforcement methods are mainly chemical grouting technology and geotextile reinforcement technology. However, the traditional chemical grouting method has a great impact on the environment, especially some grouts are difficult to degrade, and the fluidity of the grout is not good, the scope of grout reinforcement is limited, and the economic performance is low. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12C12N1/20C12R1/07
CPCC12N1/20E02D3/12
Inventor 邓温妮王悦
Owner SOUTHEAST UNIV
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