Method for improving content of soluble dietary fibers in Tibet Barley Aojiru

A technology of dietary fiber content and solubility, applied in the food field, can solve the problems of obtaining extraction rate, etc., and achieve the effect of preventing insulin secretion disorder, easy realization, and lowering cholesterol

Active Publication Date: 2019-03-29
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical methods, microbial fermentation methods, and enzymatic methods all increase the content of soluble dietary fiber by reducing the macromolecular structure, the reaction sites attacked by each method are specific, so it is difficult to achieve the best results through a single modification method. Extraction rate

Method used

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  • Method for improving content of soluble dietary fibers in Tibet Barley Aojiru

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Experimental program
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Effect test

Embodiment 1

[0030] This embodiment relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder, the steps are as follows:

[0031] Step 1, crushing the highland barley seedlings after freeze-drying repeatedly, the time of each crushing process is 5-15s, the crushing speed is 15000-25000r / min, the interval time of each crushing process is 15-25s, after 45 mesh sieve;

[0032] Step 2, in the ultra-clean workbench, inoculate the Rhizopus oryzae preserved at 4°C on the PDA slant test tube culture medium, cultivate it at 28°C for 7 days, rinse the PDA slant with sterile water after it grows mature spores, and collect the spores. Properly dilute the spore concentration at 10 8 -10 9 spores / mL, inoculate the spore suspension into the seed medium at a ratio of 1:50 (v:v) (seed medium components: glucose 0.5g, peptone 0.05g, nutrient salt solution 5mL, trace element solution 0.05mL, spit Temperature-80 0.1mL, cooled after high-pressure steam steriliza...

Embodiment 2

[0039] This embodiment relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder, the steps are as follows:

[0040] Step 1, crushing the highland barley seedlings after freeze-drying repeatedly, the time of each crushing process is 5-15s, the crushing speed is 15000-25000r / min, the interval time of each crushing process is 15-25s, after 45 mesh sieve;

[0041] Step 2, in the ultra-clean workbench, inoculate the Rhizopus oryzae preserved at 4°C on the PDA slant test tube culture medium, cultivate it at 28°C for 7 days, rinse the PDA slant with sterile water after it grows mature spores, and collect the spores. Properly dilute the spore concentration at 10 8 -10 9 spores / mL, inoculate the spore suspension into the seed medium at a ratio of 1:50 (v:v) (seed medium components: glucose 0.5g, peptone 0.05g, nutrient salt solution 5mL, trace element solution 0.05mL, spit Temperature-80 0.1mL, cooled after high-pressure steam steriliza...

Embodiment 3

[0048] This embodiment relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder, the steps are as follows:

[0049] Step 1, crushing the highland barley seedlings after freeze-drying repeatedly, the time of each crushing process is 5-15s, the crushing speed is 15000-25000r / min, the interval time of each crushing process is 15-25s, after 45 mesh sieve;

[0050] Step 2, in the ultra-clean workbench, inoculate the Rhizopus oryzae preserved at 4°C on the PDA slant test tube culture medium, cultivate it at 28°C for 7 days, rinse the PDA slant with sterile water after it grows mature spores, and collect the spores. Properly dilute the spore concentration at 10 8 -10 9 spores / mL, inoculate the spore suspension into the seed medium at a ratio of 1:50 (v:v) (seed medium components: glucose 0.5g, peptone 0.05g, nutrient salt solution 5mL, trace element solution 0.05mL, spit Temperature-80 0.1mL, cooled after high-pressure steam steriliza...

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Abstract

The invention provides a method for improving the content of soluble dietary fibers in Tibet Barley Aojiru. The method comprises the following steps: 1, preprocessing a raw material: crushing and sieving lyophilized Tibet Barley seedlings with a height of 15-30 cm; step 2, fermenting: fermenting Tibet Barley powder obtained in step 1 by using Rhizopus oryzae, and sterilizing the fermented Tibet Barley powder; 3, acidolyzing: adding an appropriate amount of water to a fermentation product obtained in step 2, and adjusting the pH value with hydrochloric acid in order to carry out acidolysis; 4,carrying out enzymatic hydrolysis: adding xylanase and cellulose to a product obtained in step 3; 5, carrying out alcohol precipitation: carrying out suction filtration on a product obtained in step 4to obtain a filtrate, dropwise adding ethanol to the filtrate, standing the obtained solution overnight, and performing centrifugation to obtain a precipitate; and 6, carrying out vacuum freezing drying: re-dissolving the obtained precipitate, carrying out rotary evaporation concentrating, and performing vacuum lyophilization. The soluble dietary fibers in the Tibet Barley Aojiru prepared in theinvention can be used as a health food or an additive thereof, and the product can reduce the postprandial blood sugar level, lower cholesterol, prevent cardiovascular diseases and resist tumors.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder. Background technique [0002] Highland barley belongs to the genus Hordeum of the Gramineae family. Because the inner and outer lemmas of the grains are separated from the caryopsis, and the grains are exposed, it is also called naked barley, which is a special type of barley. Highland barley has been planted in the Qinghai-Tibet Plateau region of my country for more than 5,000 years, and is the main source of carbohydrates for the Tibetan people. The extreme environment of the Qinghai-Tibet Plateau, such as high cold, hypoxia, and strong light, has made barley extremely resistant to stress, and has rich and unique nutrients, such as protein, polyphenols, flavonoids, mineral elements, vitamins, and dietary fiber. Although the natural environment on the Qinghai-Tibet Plateau is harsh, cen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/22
CPCA23L33/22A23V2002/00A23V2200/3262A23V2200/328A23V2200/308
Inventor 隋中泉廖樟华高海德李紫君南流南潇
Owner SHANGHAI JIAO TONG UNIV
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