A molecular marker related to boar sperm motility and total sperm count and its application

A technology of molecular markers and sperm motility, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as the decline of boar semen quality and the decrease of weaned piglets, and achieve high sensitivity and specificity good sex effect
CN109576380BActive Publication Date: 2020-12-29HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2020-12-29

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Abstract

The invention belongs to the technical field of screening of molecular markers of boars, and particularly relates to a molecular marker relative to sperm viability and a total sperm number of a boar and application. The molecular marker is cloned from a gene segment with a login number of ALGA 0001958. Through a gene chip technology, a gene is typed, screening is conducted to obtain the molecularmarker relative to characters of the sperm viability and the total sperm number of the boar, a marked nucleotide sequence is as shown in SEQ ID NO:1, A / G allele mutation exists in a base position of asite 101 of the sequence, and when nucleotide of the site 101 on the nucleotide sequence as shown in the SEQ ID NO:1 is A, the fact that the boar has higher sperm viability and a larger total sperm number is judged. The invention discloses a screening method of a molecular marker relative to a boar seminal fluid character and correlation analysis thereof. New SNP marker resources are provided formarker assisted selection of the characters of the sperm viability and the total sperm number of the boars.
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Description

technical field

[0001] The invention relates to the technical field of pig molecular marker preparation, in particular to a molecular marker related to boar sperm motility and total sperm count and its application. The molecular marker involves the use of a SNP. The molecular marker of the invention can be used for marker-assisted selection of boar sperm motility and total sperm count traits. Background technique

[0002] my country is the largest producer of pigs in the world, ranking first in both the consumption of pigs and the scale of pig breeding (Liu Wentao et al. A comparative study on the pig industry in China and the United States[J]. Chinese Journal of Animal Husbandry, 2016 , 52(06):3-7). The pig raising model has gradually developed from small-scale pig farms and individual farmers to large-scale pig farms. By 2016, the proportion of large-scale pig farms and integrated pig farming enterprises has exceeded 50% (Wang Ruinian. Analysis of the status quo of large...

Claims

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